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Immunohistochemical demonstration of CD31, HCG and receptors for estrogene on normal human placenta at term stage

Year 2008, Volume: 39 Issue: 4, 175 - 180, 01.05.2008

Abstract

Introduction: The aim of the study was to investigate the localization of CD31, hCG and the receptors which are specific for estrogene in normal human placenta. Material and Methods: Sections from 10 normal human placenta were stained with Hematoksilen-Eozin, PAS ve Masson's trichrom stain and immunolabeled with the antibodies specific for HCG(Ab-5)(Lab Vision), CD31 (Diagnostic BioSystems) and human estrogene receptor s (Diagnostic BioSystems) . RESULTS: Antibodies specific for CD31 were localised in the fetal capillary endothelial cells's cytoplasm and the endothelial cells aroud the different types of vessels in Chorion. Syncythyotrophoblasts were immunostained with hCG. There were also immunolabeling in the cytoplasm of mesenchimal cells in villi, and in some cells in chorion. Labeling for the receptors specific for Estrogene were localised in the cytoplasm of syncythiotrophoblasts, in mesenchimal cells in villi and the cytoplasm of the connective tissue cells in chorion. Conclusions: We conclude that new vessel formation is not common in villi in term placenta since immunolabeling with CD31 in mesenchimal cells at this location were not seen. Mesenchimal cells which were stained with hCG might be the cells which synthesize and secrete HCG, like syncythyotrophoblasts, or they might be primitive cells which have potential for differentiation into the cyncythiotrophoblasts. Cells labeled with the estrogene receptor specific antibodies in mesenchimal cells in chorion and in villi would be the precursor cells of syncythiotrophoblasts or other cell types carrying these type receptors.

References

  • 1.İto HH, Doughas GQ Qiu QF, Thirkill TL, Overstreet JW, Lasley BL. The relationship between trophoblast differentiation and the production ofbioactive HCG. Early Pregnancy. 1997; 3(4): 291-300
  • 2.Al-Lamki RS, Stepper JN, Burton GJ. Are human placental bed giant cells merely aggregates of small mononuclear trophoblast cells? An ultrastructural and immunocy to chemical study. Hum Reprod. 1999;14(2):496-504
  • 3.Kovalevskaya G, Genbacev O, Fisher SJ, Caceres E, O' Connor JF . Trophoblast origin of hCG isoforms: cytotrophoblasts are the primary source ofchoriocarcinoma-like hCG. 2002;194(l-2):147-55
  • 4.Barros JS, Baptista MG, Bairos VA. Human chorionic gonadotropin in human placentas from normal andpreeclamptic pregnancies. Arch Gynecol Obstet. 2002; 266(2): 67-71
  • 5.Kayisli UA, Selam B, Guzeloglu-Kayisli O, Demir R, Arici A. Human chorionic gonadotropin contributes to maternal immunotolerance and endometrial apoptosis by regulating Fas-Fas ligand system. JImmunol. 2003 ;171(5):2305-13
  • 6.Yoshida Y. Secretion of human chorionic gonadotropin in erly pregnancy. Med Mol Morphol. 2005; 38(2): 104-11
  • 7.PijnenborgR, VercruysseL, VerbistL, VanAssche FA. Interaction of interstitial trophoblast with placental bed capillaries and venules of normotensive and pre-eclamptic pregnancies. 1998;19(8):569-75
  • 8.Challier JC, Carbillon L, KacemiA, Vervelle C, Bintein T, Galtier M, Espie MJ, Uzan S. Characterization of first trimester human fetal placental vessels using immunocy to chemical markers. Cell Mol Biol(Noisy-le-grand). 2001; 47 9.Nakagawa Y, Fujimoto J, Tamaya T Placental growth by the estrogen-dependent angiogenic factors, vascular endothelial growth factor and basic fibroblast growth factor, throughout gestation. 2004;19(5):259-66
  • 10.Bukovsky A, Caudle MR, Cekanova M, Fernando RI, Wimalasena J, Faster JS, Henley DC, Elder RF. Placental expression of estrogen receptor beta and its hormone binding variant-comparison with estrogen receptor alpha and a role for estrogen receptors in asymmetric division and differentiation of estrogen-dependent cells. Reprod Biol Endocrinol. 2003, 1: 36
  • 11. Bukovsky A, Cekanova M, Caudle MR, Wimalasena J, Foster JS, Henley DC, Elder RF. Expression and localization of estrogen receptor-alpha protein in normal and abnormal term placentae and stimulation of trophoblast differentiation by estrodiol. Reprod Biol Endocrinol. 2003;l:13
  • 12.Sonoda N, Katabuchi H, Tashiro H, Ohba T, Nishimura R, Minegishi T, Okamura H. Expression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages. Placenta. 2005; 26(4):298-307
  • 13.Barros JS, Baptista MG, Bairos VA. Human chorionic gonadotropin in human placentas from normal and preeclamptic pregnancies. Arch Gynecol Obstet. 2002; 266(2): 67-71
  • 14.Tertemiz F, Kayışlı UA, Arıcı A, Demir R. Apoptosis contributes to vascular lumen formation and vascular branching in human placental vasculogenesis. Biol Reprod 2005; 72(3): 727-35
  • 15.M. Castellucci, G.Kosanke, F.Verdenelli, B.Huppertz and PKaufmann. Villous sprouting: fundamental mechanisms of human placental development.Hum Reprod Update. 2000; 6(5):425-94
  • 16.KacemiA, Vervelle C, Uzan S, Challier JC. Immunostaining of vascular, perivascular cells and stromal components in human placental villi. Cell Mol Biol (Noisy-le-grand). 1999; 45(1): 101-13
  • 17.Morrish D W, MarusykH. Localization of human chorionic gonadotropin and placental lactogen by immunogold labeling for electron microscopy technique and limitations. Microsc Res Tech. 1997; 38(1-2): 176-87
  • 18.Kumar V, Green S, Stack G, Berry M, Jin JR and Chambon P. Functional domains of the human estrogen receptor. Cell 1987; 51:941-951
  • 19.Luo D, Liu SY, Xing AY. Expression and localization of estrogen receptor ERalpha and the kinds ofERbeta mRNA on placenta. Sichuan Da XueXueBao YiXueBan. 2006Sep;37(5):769-72
  • 20.Al-Bader MD.Estrogen receptors alpha and beta in rat placenta: detection by RT-PCR, real time PCR and Western blotting. Reprod Biol Endocrinol. 2006 Mar28;4:13

Normal gelişimini tamamlamış insan plasentasında CD31, HCG ve estrojene spesifik reseptörlerin immunohistokimyasal yöntemlerle gösterilmesi

Year 2008, Volume: 39 Issue: 4, 175 - 180, 01.05.2008

Abstract

Çalışmada; CD31, hCG ve Estrojene spesifik reseptörlerin normal gelişimini tamamlamış insan plasentasındaki lokalizasyonunun araştırılması amaçlandı. Materyal ve Metod: 10 adet insan plasentasından hazırlanan kesitler; Hematoksilen-Eozin, PAS ve Masson 'un trikrom boyaları ile boyandı. Ardından; HCG(Ab-5)(Lab Vision), CDS I (Diagnostic BioSystems), insan estrojen reseptörüne spesifik antikorlar (Diagnostic BioSystems) ile indirekt immunoperoksidaz yöntemi kullanılarak işaretlendi. Bulgular: CD31 ile; fetal kapillerleri döşeyen endotelyal hücre sitoplazmaları ile; koryon içerisindeki kapillerler ve diğer tüm damarsal yapıları çevreleyen endotel hücreleri işaretlendi. Sinsityotrofoblastlar hCG ile belirgin şekilde boyandı. Villuslar içerisinde; yer yer sitoplazmaları hCG ile işaretlenmiş mezenkimal hücreler gözlendi. Koryon içerisinde de sitoplazması boyanmış hücrelere rastlandı. Estrojene spesifik reseptörlerle; sinsityotrofoblast sitoplazmalannda, villuslar içindeki mezenkimal hücrelerin bir kısmında, koryon kesitlerinde bazı bağ dokusu içerisindeki hücrelerin sitoplazmalannda işaretlenme mevcuttu. Tartışma: CD31 ile villuslar içerisinde lokalize mezenkimal hücrelerde işaretlenme gözlemleyememiz; yeni damar oluşumunun artık son dönem plasentada stimüle edilmediğini düşündürmektedir. hCG ile boyanmış mezenkimal hücrelerin varlığı bu hücrelerin; sinsityotrofoblastlar gibi hCG sentezleyip salgılayan hücreler ya da sinsityotrofoblastlar a farklılaşma potansiyeline sahip öncül hücreler olabileceği kanısındayız.Estrojen reseptörüne spesifik antikorla; koryon kesitlerinde ve villus içi mezenkimal hücrelerde gözlemlenen boyanma; sinsityotrofoblastlara dönüşmek üzere programlanmış hücreler veya bu reseptörleri taşıyan diğer tipteki hücreler olabileceklerini düşündürmektedir.

References

  • 1.İto HH, Doughas GQ Qiu QF, Thirkill TL, Overstreet JW, Lasley BL. The relationship between trophoblast differentiation and the production ofbioactive HCG. Early Pregnancy. 1997; 3(4): 291-300
  • 2.Al-Lamki RS, Stepper JN, Burton GJ. Are human placental bed giant cells merely aggregates of small mononuclear trophoblast cells? An ultrastructural and immunocy to chemical study. Hum Reprod. 1999;14(2):496-504
  • 3.Kovalevskaya G, Genbacev O, Fisher SJ, Caceres E, O' Connor JF . Trophoblast origin of hCG isoforms: cytotrophoblasts are the primary source ofchoriocarcinoma-like hCG. 2002;194(l-2):147-55
  • 4.Barros JS, Baptista MG, Bairos VA. Human chorionic gonadotropin in human placentas from normal andpreeclamptic pregnancies. Arch Gynecol Obstet. 2002; 266(2): 67-71
  • 5.Kayisli UA, Selam B, Guzeloglu-Kayisli O, Demir R, Arici A. Human chorionic gonadotropin contributes to maternal immunotolerance and endometrial apoptosis by regulating Fas-Fas ligand system. JImmunol. 2003 ;171(5):2305-13
  • 6.Yoshida Y. Secretion of human chorionic gonadotropin in erly pregnancy. Med Mol Morphol. 2005; 38(2): 104-11
  • 7.PijnenborgR, VercruysseL, VerbistL, VanAssche FA. Interaction of interstitial trophoblast with placental bed capillaries and venules of normotensive and pre-eclamptic pregnancies. 1998;19(8):569-75
  • 8.Challier JC, Carbillon L, KacemiA, Vervelle C, Bintein T, Galtier M, Espie MJ, Uzan S. Characterization of first trimester human fetal placental vessels using immunocy to chemical markers. Cell Mol Biol(Noisy-le-grand). 2001; 47 9.Nakagawa Y, Fujimoto J, Tamaya T Placental growth by the estrogen-dependent angiogenic factors, vascular endothelial growth factor and basic fibroblast growth factor, throughout gestation. 2004;19(5):259-66
  • 10.Bukovsky A, Caudle MR, Cekanova M, Fernando RI, Wimalasena J, Faster JS, Henley DC, Elder RF. Placental expression of estrogen receptor beta and its hormone binding variant-comparison with estrogen receptor alpha and a role for estrogen receptors in asymmetric division and differentiation of estrogen-dependent cells. Reprod Biol Endocrinol. 2003, 1: 36
  • 11. Bukovsky A, Cekanova M, Caudle MR, Wimalasena J, Foster JS, Henley DC, Elder RF. Expression and localization of estrogen receptor-alpha protein in normal and abnormal term placentae and stimulation of trophoblast differentiation by estrodiol. Reprod Biol Endocrinol. 2003;l:13
  • 12.Sonoda N, Katabuchi H, Tashiro H, Ohba T, Nishimura R, Minegishi T, Okamura H. Expression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages. Placenta. 2005; 26(4):298-307
  • 13.Barros JS, Baptista MG, Bairos VA. Human chorionic gonadotropin in human placentas from normal and preeclamptic pregnancies. Arch Gynecol Obstet. 2002; 266(2): 67-71
  • 14.Tertemiz F, Kayışlı UA, Arıcı A, Demir R. Apoptosis contributes to vascular lumen formation and vascular branching in human placental vasculogenesis. Biol Reprod 2005; 72(3): 727-35
  • 15.M. Castellucci, G.Kosanke, F.Verdenelli, B.Huppertz and PKaufmann. Villous sprouting: fundamental mechanisms of human placental development.Hum Reprod Update. 2000; 6(5):425-94
  • 16.KacemiA, Vervelle C, Uzan S, Challier JC. Immunostaining of vascular, perivascular cells and stromal components in human placental villi. Cell Mol Biol (Noisy-le-grand). 1999; 45(1): 101-13
  • 17.Morrish D W, MarusykH. Localization of human chorionic gonadotropin and placental lactogen by immunogold labeling for electron microscopy technique and limitations. Microsc Res Tech. 1997; 38(1-2): 176-87
  • 18.Kumar V, Green S, Stack G, Berry M, Jin JR and Chambon P. Functional domains of the human estrogen receptor. Cell 1987; 51:941-951
  • 19.Luo D, Liu SY, Xing AY. Expression and localization of estrogen receptor ERalpha and the kinds ofERbeta mRNA on placenta. Sichuan Da XueXueBao YiXueBan. 2006Sep;37(5):769-72
  • 20.Al-Bader MD.Estrogen receptors alpha and beta in rat placenta: detection by RT-PCR, real time PCR and Western blotting. Reprod Biol Endocrinol. 2006 Mar28;4:13
There are 19 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Ranan Gülhan Aktaş This is me

Aynure Öztekin This is me

Ülkü Bayar This is me

Coşkun Öztekin This is me

Publication Date May 1, 2008
Published in Issue Year 2008 Volume: 39 Issue: 4

Cite

APA Aktaş, R. G., Öztekin, A., Bayar, Ü., Öztekin, C. (2008). Normal gelişimini tamamlamış insan plasentasında CD31, HCG ve estrojene spesifik reseptörlerin immunohistokimyasal yöntemlerle gösterilmesi. Zeynep Kamil Tıp Bülteni, 39(4), 175-180. https://doi.org/10.16948/zktb.63884
AMA Aktaş RG, Öztekin A, Bayar Ü, Öztekin C. Normal gelişimini tamamlamış insan plasentasında CD31, HCG ve estrojene spesifik reseptörlerin immunohistokimyasal yöntemlerle gösterilmesi. Zeynep Kamil Tıp Bülteni. May 2008;39(4):175-180. doi:10.16948/zktb.63884
Chicago Aktaş, Ranan Gülhan, Aynure Öztekin, Ülkü Bayar, and Coşkun Öztekin. “Normal gelişimini tamamlamış Insan plasentasında CD31, HCG Ve Estrojene Spesifik reseptörlerin Immunohistokimyasal yöntemlerle gösterilmesi”. Zeynep Kamil Tıp Bülteni 39, no. 4 (May 2008): 175-80. https://doi.org/10.16948/zktb.63884.
EndNote Aktaş RG, Öztekin A, Bayar Ü, Öztekin C (May 1, 2008) Normal gelişimini tamamlamış insan plasentasında CD31, HCG ve estrojene spesifik reseptörlerin immunohistokimyasal yöntemlerle gösterilmesi. Zeynep Kamil Tıp Bülteni 39 4 175–180.
IEEE R. G. Aktaş, A. Öztekin, Ü. Bayar, and C. Öztekin, “Normal gelişimini tamamlamış insan plasentasında CD31, HCG ve estrojene spesifik reseptörlerin immunohistokimyasal yöntemlerle gösterilmesi”, Zeynep Kamil Tıp Bülteni, vol. 39, no. 4, pp. 175–180, 2008, doi: 10.16948/zktb.63884.
ISNAD Aktaş, Ranan Gülhan et al. “Normal gelişimini tamamlamış Insan plasentasında CD31, HCG Ve Estrojene Spesifik reseptörlerin Immunohistokimyasal yöntemlerle gösterilmesi”. Zeynep Kamil Tıp Bülteni 39/4 (May 2008), 175-180. https://doi.org/10.16948/zktb.63884.
JAMA Aktaş RG, Öztekin A, Bayar Ü, Öztekin C. Normal gelişimini tamamlamış insan plasentasında CD31, HCG ve estrojene spesifik reseptörlerin immunohistokimyasal yöntemlerle gösterilmesi. Zeynep Kamil Tıp Bülteni. 2008;39:175–180.
MLA Aktaş, Ranan Gülhan et al. “Normal gelişimini tamamlamış Insan plasentasında CD31, HCG Ve Estrojene Spesifik reseptörlerin Immunohistokimyasal yöntemlerle gösterilmesi”. Zeynep Kamil Tıp Bülteni, vol. 39, no. 4, 2008, pp. 175-80, doi:10.16948/zktb.63884.
Vancouver Aktaş RG, Öztekin A, Bayar Ü, Öztekin C. Normal gelişimini tamamlamış insan plasentasında CD31, HCG ve estrojene spesifik reseptörlerin immunohistokimyasal yöntemlerle gösterilmesi. Zeynep Kamil Tıp Bülteni. 2008;39(4):175-80.