Abstract
A mature oocyte and a mature sperm are neededfor intracytoplasmic sperm injection (ICSI), a common method which is used in Assisted Reproductive Technologies. There are recent studies for being able to develope the best method(s) for being to help to these couples. ICSI was done with immature spermatogenetic cells in several cases in past. Howerever; fertilization and pregnancy rates were very low in these cases. This is a case report about a couple applied for infertility treatment to our center. "Elongated" spermatids were used for ICSI since mature sperm could not be found in tissue. The results are discussed in the study.
References
- 1- Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, ve ark. Pregnancies after testicular sperm extraction and intracytöplasmic sperm injection in nonobstructive azoospermia. Hum Reprod. 1995; 10:1457-1460 2- Tachibana M, Terada Y, Ogonuki N, Ugajin T, Ogura A ve ark. Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes. Mol Reprod Dev. 2009 Mar;76(3):270-7
- 3- Kahraman S, Polat G, Samli M, Sözen E, Özgün O.D, Dirican K, ve ark. Multiple pregnancies obtained by testicular spermatid injection in combination with intracytoplazmic sperm injection. Hum Reprod. 1998;13:104-110 4- Ghazzawi IM, Alhasani S, taher M, Sous o S. Reproductive capacity of round spermatids compared with mature spermatozoa in a population of azoospermic men. Hum Reprod. 1999; Mar; 14(3) :736-740 5- Vicdan K, Isık A.Z, Delilbasi L. Development of blastocyst-stage embryos after round spermatid injection in patients with complete spermiogenesis failure. J Assist Reprod Genet. 2001; Feb; 18(2) :78- 86 6- Tachibana M, Terada Y, Ogonuki N, Ugajin T, Ogura A, Murakami T, ve ark. Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes. Mol Reprod Dev. 2009; Mar;76(3):270-277 7- Al- Hasani S, Ludwig M, Palermo 1, Küpker W, Sandmann J, Johannisson R, ve ark. intracytöplasmic injection of round and elongated spermatids from azoospermic patients: results and review. Hum Reprod. 1999; Sep; 14 Suppl 1:97- 107
Abstract
Üremeye Yardımcı Tedaviler Merkezlerinde en yaygın kullanılan yöntemlerden olan intrasitoplazmik sperm enjeksiyonu (ICSI) işlemi için olgun bir yumurtaya ve olgun bir sperm hücresine ihtiyaç vardır. Anne ve baba adaylarından her zaman gelişimini tamamlamış oosit ve spermler elde edilememektedir. Bu durumlarda çiftlere yardımcı olabilmek amacı ile yöntemler geliştirilmeye çalışılmaktadır. Çok az sayıda olguda; henüz gelişimini tamamlayamamış spermatogenetik seri hücreleri ile ICSI işlemi yapılmış ve bunlardan da az oranda fertilizasyon ve gebelik elde edilebilmiştir. Merkezimize başvuran bir çifte de; TESE dokusunda olgun spermler bulunamaması nedeni ile "elongated" spermatidlerle ICSI işlemi yapılmış ve sonuçlar bu olgu sunumunda tartışılmıştır.
References
- 1- Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, ve ark. Pregnancies after testicular sperm extraction and intracytöplasmic sperm injection in nonobstructive azoospermia. Hum Reprod. 1995; 10:1457-1460 2- Tachibana M, Terada Y, Ogonuki N, Ugajin T, Ogura A ve ark. Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes. Mol Reprod Dev. 2009 Mar;76(3):270-7
- 3- Kahraman S, Polat G, Samli M, Sözen E, Özgün O.D, Dirican K, ve ark. Multiple pregnancies obtained by testicular spermatid injection in combination with intracytoplazmic sperm injection. Hum Reprod. 1998;13:104-110 4- Ghazzawi IM, Alhasani S, taher M, Sous o S. Reproductive capacity of round spermatids compared with mature spermatozoa in a population of azoospermic men. Hum Reprod. 1999; Mar; 14(3) :736-740 5- Vicdan K, Isık A.Z, Delilbasi L. Development of blastocyst-stage embryos after round spermatid injection in patients with complete spermiogenesis failure. J Assist Reprod Genet. 2001; Feb; 18(2) :78- 86 6- Tachibana M, Terada Y, Ogonuki N, Ugajin T, Ogura A, Murakami T, ve ark. Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes. Mol Reprod Dev. 2009; Mar;76(3):270-277 7- Al- Hasani S, Ludwig M, Palermo 1, Küpker W, Sandmann J, Johannisson R, ve ark. intracytöplasmic injection of round and elongated spermatids from azoospermic patients: results and review. Hum Reprod. 1999; Sep; 14 Suppl 1:97- 107
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | May 1, 2009 |
| Published in Issue | Year 2009 Volume: 40 Issue: 4 |