Investigation on Differentiation of Adventitious Root Primordia in Grapevine (Vitis vinifera L.)

Öz

The research was carried out as a basic study to determine localization of adventitious roots differentiated in the stemtissues of the grapevine (Vitis vinifera L.) at the microscopic level. The cuttings of Cabernet Sauvignon kept in rooting medium were grouped by their thickness (more or less than 1 cm in diameter) and 4.5 cm long basal parts were kept in FAA (formalin 10%, ethanol 50% and glacial acetic acid 5%). Samples cut into sections with a thickness of 60-90 μm were stained with safranin. It was determined that the adventitious roots are differentiated from two different regions of the stem tissues. In the thin samples (diameter <1 cm), root primordia formed in the regions close to the outside of the conductive tissue system, while in the older cuttings (diameter > 1cm) they were differentiated from the cells in more inner region close to pith. Callus, formed as an irregular cell mass, is developed from the phloem region adjacent to the cambium. Formation of adventitious root and callus, which are shaped of independently each other, is within the same time frame.

Anahtar Kelimeler

• grapevine
• staining
• cutting
• safranin
• differentiation

Asmada (Vitis vinifera L.) Adventif Kök Taslaklarının Farklılaşmasının İncelenmesi

Öz

Araştırma, asmada (Vitis vinifera L.) adventif köklerin gövde dokularından farklılaşmasını mikroskobik düzeyde belirlemek amacına yönelik olarak temel bir çalışma niteliğinde gerçekleştirilmiştir. Köklendirme ortamında bekletilen Cabernet Sauvignon çeşidinin çelikleri kalınlıklarına (çap 1 cm’nin altı veya üstü) göre ayrılıp bazal kısımlarından 4-5 cm uzunluğunda kesilerek FAA (formalin %10, etanol %50 ve glasiyel asetik asit %5) ortamında muhafaza edilmiştir. 60-90 μm kalınlığında kesitlere ayrılan örnekler safranin ile boyanmıştır. Adventif köklerin gövde kesitini oluşturan dokuların iki farklı bölgesinden farklılaştığı tespit edilmiştir. İnce dal parçalarında (çap<1cm) kök taslakları, iletken doku sisteminin dış tarafına yakın hücre gruplarının bulunduğu bölgelerden, kalın dal parçalarında (çap>1cm) ise daha içte ve öze yakın bölgelerdeki hücre gruplarından farklılaşmıştır. Düzensiz bir hücre yığını halinde oluşan kallus, kambiyumuma bitişik floem bölgesinden gelişmektedir. Birbirlerinden bağımsız şekillenen adventif kök ve kallus oluşumunun aynı zaman aralığında olduğu gözlenmiştir.

Anahtar Kelimeler

• Asma
• boyama
• çelik
• safranin
• farklılaşma

Kaynakça

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