Kısa Süreli Polen Muhafaza Yöntemleri Kullanılarak Sera Şartlarında Mısır Bitkilerinde Tohumluk Üretimi

Year 2022, Issue: 374, 15 - 23, 30.04.2022

Nur Yüksel , Melih Köstekçi , Ahmet Serdar Turcan , Fatih Kahrıman

https://doi.org/10.33724/zm.978322

Abstract

Bu çalışma, sera şartlarında yetiştirilen mısır hat ve hibritlerinin farklı polen muhafaza yöntemleri ile tohum üretimini incelemek amacıyla yürütülmüştür. Araştırmada altı farklı genotip (IHO, B73, HYA, HYAxB73, MAY66, IND2) materyal olarak kullanılmıştır. Sera denemesi 2020 yılı Eylül-Mart ayları arasında yürütülmüştür. Her materyalden en az 30 fide yetiştirilerek saksılara şaşırtılmıştır. Polen muhafaza yöntemi olarak üç ayrı yöntem (Tepe püskülü muhafaza, +4 °C polen muhafaza, -20 °C polen muhafaza) kıyaslanmış ve 3’er gün aralıklarla 9 günlük sürede tozlanma işlemleri gerçekleştirilmiştir. Her tozlanma işleminden önce kullanılan polen örneğinde canlılık testi yapılarak muhafaza yöntemlerine göre polen canlılığındaki değişimi izlenmiştir. Ayrıca sera şartlarında bitkilerin gelişimlerini izlemek amacıyla bitki boyu (cm), tepe püskülü boyu (cm) ve tepe püskülü yan dal sayısı (adet) ölçümleri alınmıştır. Çalışma sonuçları mısır genotiplerinden sera şartlarında toplanan polen örneklerinin canlılık süresinin kullanılan yönteme göre değiştiğini göstermiştir. Tohum bağlama ve sera şartlarında gelişim bakımından da genotipler arasında önemli farklılıklar olduğu belirlenmiştir.

Keywords

Polen Muhafaza, Zea mays, Canlılık, Tohum Oluşumu, Mısır ıslahı.

Supporting Institution

TÜBİTAK

Project Number

1919B012000581

Thanks

Bu çalışma TÜBİTAK-BİDEB tarafından TÜBİTAK 2209-A 1919B012000581 no’lu proje kapsamında desteklenmiştir. Desteklerinden ötürü TÜBİTAK'a teşekkür ederiz.

Seed Production in Maize Plants under Greenhouse Conditions using Short-Time Polen Conservation Methods

Abstract

This study was carried out to investigate the seed production of maize lines and hybrids grown under greenhouse conditions with different pollen conservation methods. Six different genotypes (IHO, B73, HYA, HYAxB73, MAY66, IND2) were used as materials. The greenhouse experiment was carried out between September and March 2020. At least 30 seedlings were grown from each material and transplanted into pots. Three different pollen conservation methods (Tassel preservation, +4 °C pollen storage, -20 °C pollen storage) were compared and pollination processes were carried out in 9 days with 3-day intervals. Viability test was performed on the pollen sample used before each pollination process, and the change in pollen viability was monitored according to the preservation methods. Additionaly, plant height (cm), tassel length (cm) and tassel side branch number (piece) measurements were taken in order to monitor the development of plants under greenhouse conditions. The results of the study showed that the viability of the pollen samples collected under greenhouse conditions of the maize genotypes varied according to the method used. It was determined that there were significant differences between genotypes in terms of seed setting and growth in greenhouse conditions.

Keywords

Polen Conservation, Zea mays, Viability, Seed Set, Maize Breeding

Project Number

1919B012000581

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