Öz
Amaç: Çalışmanın amacı geleneksel
olarak halk hekimliğinde kullanılan bazı meyvelerin antiproliferatif aktivitelerini
araştırmak olup, Fructus cynosbati (Kuşburnu), Rubus fruticosus (Böğürtlen), Sorbus
torminalis (Akçaağaç yapraklı üvez), Vaccinium
myrtillus (Yabanmersini) ve Vitis
labrusca (Kokulu üzüm)’ün meyveleri çalışmada kullanılmıştır.
Materyal-Metod: Meyvelerin
metanol özütleri elde edilerek, çeşitli konsantrasyonlarda ARPE-19 (normal) ve
HeLa (kanser) hücre kültür ortamlarına eklenmiştir. Pozitif ve negatif kontrol
olarak sırasıyla, Taxol ve DMSO kullanılmış olup, özütlerin sitotoksik etkisi,
48 saatlik hücre inkübasyon süresinden sonra MTT testi ile değerlendirilmiştir.
Verilerin istatistiksel analizi, unpaired t-testi kullanılarak gerçekleştirilmiş
olup, p değeri 0.05’ten küçük olan sonuçlar anlamlı olarak kabul edilmiştir.
Bulgular: V. labrusca her iki hücre hattına
karşı doza bağlı sitotoksik aktivite göstermiştir. Ayrıca V. myrtillus ve
R. fruticosus, özütlerinin
artan konsantrasyonlarda her iki hücre hattında orta derecede sitotoksik etkili
olduğu saptanmıştır. Buna ek olarak, F. cynosbati ve S. torminalis özütlerinin sadece
HeLa hücrelerinde canlılığı doza bağlı olarak azalttığı görülmüş ve sonuçlar istatistiksel
olarak anlamlı bulunmuştur (p <0.05). Fakat çalışmada S. torminalis özütünün HeLa hücrelerine karşı en
etkili özüt olduğu saptanmıştır.
Sonuç: Bu sonuçlar, S. torminalis methanol özütünün servikal kanser hücrelerine karşı
in vitro koşullarda güçlü antiproliferatif etkisinin olduğunu göstermiştir.
Sonuç önemli olup, S. torminalis methanol özütü içindeki spesifik biyoaktif bileşenlerinin izolasyonu ve
purifikasyonu için yeni çalışmalara ihtiyaç vardır.
Anahtar Kelimeler
Destekleyen Kurum
Recep Tayyip Erdoğan Üniversitesi BAP
Proje Numarası
BAP-2015.53001.106.01.04
Kaynakça
- 1. Xu J, Liu XS, Zhou SF, et al. Combination of immunotherapy with anaerobic bacteria for immunogene therapy of solid tumours. Gene Terapy and Molecular Biology. 2009; 13(1): 36–52.
- 2. Joseph A, Dhanraj K, Ramalingam S, et al. Treatıng Cervical Cancer With Herbs: A Revıew. Asian J Pharm Clin Res. 2018; 11(7): 4-10.
- 3. Jeong WS, Kim IW, Hu R, et al. Modulatory properties of various natural chemopreventive agents on the activation of NF-B signaling pathway. Pharmaceutical Research. 2004; 21(4): 661–670.
- 4. Sakarkar DM, Deshmukh VN. Ethnopharmacological review of traditional medicinal plants for anticancer activity. International Journal of PharmTech Research. 2011; 3(1): 298–308.
- 5. Zhang Y, Zhang Z, Yang Y, et al. Diuretic Activity of Rubus idaeus L (Rosaceae) in Rats. Trop. J. Pharm. Res. 2011; 10: 243–248.
- 6. Konıshı T, Shımada Y, Nagao T, et al. Antiproliferative Sesquiterpene Lactones from the Roots of Inula helenium. Biol. Pharm. Bull. 2002; 25(10): 1370-1372.
- 7. Velioğlu S, Poyrazoğlu ES. Kuşburnu bitkisinin insan beslenmesi ve sağlığı açısından önemi. Tarım Orman ve Köyişleri Bakanlığı Dergisi, 1988; 32: 36-37.
- 8. Jennings DL. Raspberries and Blackberries: Their Breeding, Diseases and Growth. Academic Press, 1988, London.
- 9. Benvenuti S, Pellati F, Melegari M, et al. Polyphenols, Anthocyanins, Ascorbic Acid, and Radical Scavenging Activity of Rubus, Ribes, and Aronia. Journal of Food Science, 2004; 69(3).
- 10. Byers T, Perry G. Dietary carotenes, vitamin C and vitamin E as protective antioxidants in human cancers. Ann Rev Nutr. 1992; (12):139–59.
- 11. Blassan PG, Heidi A, Nanjundaswamy MH. Anticancer effects elicited by combination of Rubus extract with phthalocyanine photosensitiser on MCF-7 human breast cancer cells. Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, oornfontein, 2017, Johannesburg, South Africa.
- 12. Razina TG, Zueva EP, Ulrich AV, et al. Antitumor Effects of Sorbus aucuparia L. Extract HighlySaturated with Anthocyans and Their Mechanisms. Byulleten’ Eksperimental’noi Biology Meditsiny, 2016;162(7):107-112.
- 13. You NO, Soojung J, Hyun JP, et al. Sorbus rufopilosa Extract Exhibits Antioxidant and Anticancer Activities by Including Cell Cycle Arrest and Apoptosis in Human Colon Adenocarcinoma HT29 Cells. Journal of Cancer Prevention. 2016; 21(4): 249-256.
- 14. Adams LS, Phung S, Yee N, et al. Blueberry phytochemicals inhibit growth and metastatic potential of MDA-MB-231 breast cancer cells through modulation of the phosphatidylinositol 3-kinase pathway. Cancer Res. 2010; 70:3594–3605.
- 15. Samad NB, Debnath T, Ye M, et al. In vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosumL.) extracts. Asian Pac. J. Trop. Biomed. 2014; 4: 807–815.
- 16. Schantz M, Mohn C, Baum M, et al. Antioxidative efficiency of an anthocyanin rich bilberry extract in the human colon tumor cell lines Caco-2 and HT-29. J. Berry Res. 2010; 1: 25–33.
- 17. Liu J, Zhang W, Jing H, et al. Bog bilberry (Vaccinium uliginosumL.) extract reduces cultured Hep-G2, Caco-2, and 3T3-L1 cell viability, affects cell cycle progression, and has variable effects on membrane permeability. J. Food Sci. 2010; 75: 103–107.
- 18. Srivastava A, Akoh CC, Fischer J, et al. Effect of anthocyanin fractions from selected cultivars of Georgia-grown blueberries on apoptosis and phase II enzymes. J. Agric. Food Chem. 2007; 55: 3180–3185.
- 19. Sung J, Lee J. Antioxidant and Antiproliferative Activities of Grape Seeds fromDifferent Cultivars. Food Sci. Biotechnol.2010; 19(2): 321-326.
- 20. Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65: 55-63.
- 21. Safarzade E, Sandoghchian S S, Baradaran B. Herbal Medicine as Inducers of Apoptosis in Cancer Treatment. Advanced Pharmaceutical Bulletin. 2014; 4: 421-427.
- 22. Park H, Park H, Chung TW, et al. Effect of Sorbus Commixta on the Invasion and Migration of Human Hepatocellular Carcinoma Hep3b Cells, Internatıonal Journal of Molecular Medicine. 2017; 40: 483-490.
Öz
Objective: The aim of this study was to evaluate the
antiproliferative activities of some the fruits, traditionally used in folk
medicine. Therefore, the
fruits of Fructus cynosbati (Rosehip), Rubus fruticosus (Blackberry), Sorbus torminalis (Wild service tree), Vaccinium myrtillus (Blueberry) and Vitis labrusca (Isabella grape) were used in the
present study.
Material and Methods: Methanol extracts of the
fruits were obtained and added to ARPE-19 (no-trasformed) and HeLa
(transformed) culture media at various concentrations. Taxol and DMSO were used
as positive and negative controls, respectively. After 48 h of cell incubation,
the cytotoxic effect of the extracts was assessed by MTT. Cell viability values
were calculated for each group. The statistical analysis of the data was
performed using unpaired t-test. p values less than 0.05 were considered
significant.
Findings: V. labrusca showed dose dependent cytotoxic activity against both cell lines. In
additions, V. myrtillus and R. fruticosus extracts
exhibited moderate cytotoxic effect on both cell lines at higher concentrations.
Extracts of F. cynosbati and S. torminalis were found to decrease the cell
viability in a dose-dependent manner in HeLa cells and the results were
statistically significant (p <0.05). Additionally, S. torminalis extract was found to be the most effective
extract against the HeLa cells.
Conclusion: These results demonsrated that S. torminalis methanol extract has a potent in vitro antiproliferative effect
toward the human cervical cancer cell line HeLa. Further work needs to be done
on isolation and purification of specific bioactive compounds in S. torminalis methanol extract.
Anahtar Kelimeler
Proje Numarası
BAP-2015.53001.106.01.04
Kaynakça
- 1. Xu J, Liu XS, Zhou SF, et al. Combination of immunotherapy with anaerobic bacteria for immunogene therapy of solid tumours. Gene Terapy and Molecular Biology. 2009; 13(1): 36–52.
- 2. Joseph A, Dhanraj K, Ramalingam S, et al. Treatıng Cervical Cancer With Herbs: A Revıew. Asian J Pharm Clin Res. 2018; 11(7): 4-10.
- 3. Jeong WS, Kim IW, Hu R, et al. Modulatory properties of various natural chemopreventive agents on the activation of NF-B signaling pathway. Pharmaceutical Research. 2004; 21(4): 661–670.
- 4. Sakarkar DM, Deshmukh VN. Ethnopharmacological review of traditional medicinal plants for anticancer activity. International Journal of PharmTech Research. 2011; 3(1): 298–308.
- 5. Zhang Y, Zhang Z, Yang Y, et al. Diuretic Activity of Rubus idaeus L (Rosaceae) in Rats. Trop. J. Pharm. Res. 2011; 10: 243–248.
- 6. Konıshı T, Shımada Y, Nagao T, et al. Antiproliferative Sesquiterpene Lactones from the Roots of Inula helenium. Biol. Pharm. Bull. 2002; 25(10): 1370-1372.
- 7. Velioğlu S, Poyrazoğlu ES. Kuşburnu bitkisinin insan beslenmesi ve sağlığı açısından önemi. Tarım Orman ve Köyişleri Bakanlığı Dergisi, 1988; 32: 36-37.
- 8. Jennings DL. Raspberries and Blackberries: Their Breeding, Diseases and Growth. Academic Press, 1988, London.
- 9. Benvenuti S, Pellati F, Melegari M, et al. Polyphenols, Anthocyanins, Ascorbic Acid, and Radical Scavenging Activity of Rubus, Ribes, and Aronia. Journal of Food Science, 2004; 69(3).
- 10. Byers T, Perry G. Dietary carotenes, vitamin C and vitamin E as protective antioxidants in human cancers. Ann Rev Nutr. 1992; (12):139–59.
- 11. Blassan PG, Heidi A, Nanjundaswamy MH. Anticancer effects elicited by combination of Rubus extract with phthalocyanine photosensitiser on MCF-7 human breast cancer cells. Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, oornfontein, 2017, Johannesburg, South Africa.
- 12. Razina TG, Zueva EP, Ulrich AV, et al. Antitumor Effects of Sorbus aucuparia L. Extract HighlySaturated with Anthocyans and Their Mechanisms. Byulleten’ Eksperimental’noi Biology Meditsiny, 2016;162(7):107-112.
- 13. You NO, Soojung J, Hyun JP, et al. Sorbus rufopilosa Extract Exhibits Antioxidant and Anticancer Activities by Including Cell Cycle Arrest and Apoptosis in Human Colon Adenocarcinoma HT29 Cells. Journal of Cancer Prevention. 2016; 21(4): 249-256.
- 14. Adams LS, Phung S, Yee N, et al. Blueberry phytochemicals inhibit growth and metastatic potential of MDA-MB-231 breast cancer cells through modulation of the phosphatidylinositol 3-kinase pathway. Cancer Res. 2010; 70:3594–3605.
- 15. Samad NB, Debnath T, Ye M, et al. In vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosumL.) extracts. Asian Pac. J. Trop. Biomed. 2014; 4: 807–815.
- 16. Schantz M, Mohn C, Baum M, et al. Antioxidative efficiency of an anthocyanin rich bilberry extract in the human colon tumor cell lines Caco-2 and HT-29. J. Berry Res. 2010; 1: 25–33.
- 17. Liu J, Zhang W, Jing H, et al. Bog bilberry (Vaccinium uliginosumL.) extract reduces cultured Hep-G2, Caco-2, and 3T3-L1 cell viability, affects cell cycle progression, and has variable effects on membrane permeability. J. Food Sci. 2010; 75: 103–107.
- 18. Srivastava A, Akoh CC, Fischer J, et al. Effect of anthocyanin fractions from selected cultivars of Georgia-grown blueberries on apoptosis and phase II enzymes. J. Agric. Food Chem. 2007; 55: 3180–3185.
- 19. Sung J, Lee J. Antioxidant and Antiproliferative Activities of Grape Seeds fromDifferent Cultivars. Food Sci. Biotechnol.2010; 19(2): 321-326.
- 20. Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65: 55-63.
- 21. Safarzade E, Sandoghchian S S, Baradaran B. Herbal Medicine as Inducers of Apoptosis in Cancer Treatment. Advanced Pharmaceutical Bulletin. 2014; 4: 421-427.
- 22. Park H, Park H, Chung TW, et al. Effect of Sorbus Commixta on the Invasion and Migration of Human Hepatocellular Carcinoma Hep3b Cells, Internatıonal Journal of Molecular Medicine. 2017; 40: 483-490.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Klinik Tıp Bilimleri (Diğer) |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | BAP-2015.53001.106.01.04 |
| Yayımlanma Tarihi | 29 Ağustos 2019 |
| Kabul Tarihi | 9 Ağustos 2019 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 3 Sayı: 2 |
Cited By
YABANMERSİNİ (VACCİNİUM MYRTİLLUS L.) MEYVE ÖZÜ'NÜN MİDE KANSERİ, AGS HÜCRE HATTI ÜZERİNDE ANTİOKSİDAN AKTİVİTESİ VE ANTİ-KANSER ETKİLERİ
Ankara Universitesi Eczacilik Fakultesi Dergisi
https://doi.org/10.33483/jfpau.1069607
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