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Yıl 2019, Cilt 3 , Sayı 2, Sayfalar 111 - 116 2019-08-29

Farklı Meyvelerin İnsan Servikal Kanser Hücreleri Üzerindeki Sitotoksik Etkileri
In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells

Saliha Ekşi [1] , Sena Şahin Aktura [2] , Nebahat Ejder [3] , Kazım Şahin [4]


Amaç: Çalışmanın amacı geleneksel olarak halk hekimliğinde kullanılan bazı meyvelerin antiproliferatif aktivitelerini araştırmak olup,  Fructus cynosbati (Kuşburnu), Rubus fruticosus (Böğürtlen), Sorbus torminalis (Akçaağaç yapraklı üvez), Vaccinium myrtillus (Yabanmersini) ve Vitis labrusca (Kokulu üzüm)’ün meyveleri çalışmada kullanılmıştır.

Materyal-Metod: Meyvelerin metanol özütleri elde edilerek, çeşitli konsantrasyonlarda ARPE-19 (normal) ve HeLa (kanser) hücre kültür ortamlarına eklenmiştir. Pozitif ve negatif kontrol olarak sırasıyla, Taxol ve DMSO kullanılmış olup, özütlerin sitotoksik etkisi, 48 saatlik hücre inkübasyon süresinden sonra MTT testi ile değerlendirilmiştir. Verilerin istatistiksel analizi, unpaired t-testi kullanılarak gerçekleştirilmiş olup, p değeri 0.05’ten küçük olan sonuçlar anlamlı olarak kabul edilmiştir.

Bulgular: V. labrusca her iki hücre hattına karşı doza bağlı sitotoksik aktivite göstermiştir.  Ayrıca V. myrtillus ve R. fruticosus, özütlerinin artan konsantrasyonlarda her iki hücre hattında orta derecede sitotoksik etkili olduğu saptanmıştır. Buna ek olarak, F. cynosbati ve S. torminalis özütlerinin sadece HeLa hücrelerinde canlılığı doza bağlı olarak azalttığı görülmüş ve sonuçlar istatistiksel olarak anlamlı bulunmuştur (p <0.05). Fakat çalışmada S. torminalis özütünün HeLa hücrelerine karşı en etkili özüt olduğu saptanmıştır.

Sonuç: Bu sonuçlar, S. torminalis methanol özütünün servikal kanser hücrelerine karşı in vitro koşullarda güçlü antiproliferatif etkisinin olduğunu göstermiştir. Sonuç önemli olup, S. torminalis methanol özütü içindeki spesifik biyoaktif bileşenlerinin izolasyonu ve purifikasyonu için yeni çalışmalara ihtiyaç vardır.

Objective: The aim of this study was to evaluate the antiproliferative activities of some the fruits, traditionally used in folk medicine. Therefore, the fruits of Fructus cynosbati (Rosehip), Rubus fruticosus (Blackberry), Sorbus torminalis (Wild service tree), Vaccinium myrtillus (Blueberry) and Vitis labrusca (Isabella grape) were used in the present study.

Material and Methods: Methanol extracts of the fruits were obtained and added to ARPE-19 (no-trasformed) and HeLa (transformed) culture media at various concentrations. Taxol and DMSO were used as positive and negative controls, respectively. After 48 h of cell incubation, the cytotoxic effect of the extracts was assessed by MTT. Cell viability values ​​were calculated for each group. The statistical analysis of the data was performed using unpaired t-test. p values ​​less than 0.05 were considered significant.

Findings: V. labrusca showed dose dependent cytotoxic activity against both cell lines. In additions, V. myrtillus and R. fruticosus extracts exhibited moderate cytotoxic effect on both cell lines at higher concentrations. Extracts of F. cynosbati and S. torminalis were found to decrease the cell viability in a dose-dependent manner in HeLa cells and the results were statistically significant (p <0.05). Additionally, S. torminalis extract was found to be the most effective extract against the HeLa cells. 

Conclusion: These results demonsrated that S. torminalis methanol extract has a potent in vitro antiproliferative effect toward the human cervical cancer cell line HeLa. Further work needs to be done on isolation and purification of specific bioactive compounds in S. torminalis methanol extract.

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Birincil Dil en
Konular Tıbbi Araştırmalar Deneysel
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-8818-7855
Yazar: Saliha Ekşi (Sorumlu Yazar)
Kurum: RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0003-2407-6942
Yazar: Sena Şahin Aktura
Kurum: RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ, TIP FAKÜLTESİ

Orcid: 0000-0001-9557-2033
Yazar: Nebahat Ejder
Kurum: RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ, TIP FAKÜLTESİ

Orcid: 0000-0002-6459-1850
Yazar: Kazım Şahin
Kurum: RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ, TIP FAKÜLTESİ

Destekleyen Kurum Recep Tayyip Erdoğan Üniversitesi BAP
Proje Numarası BAP-2015.53001.106.01.04
Tarihler

Yayımlanma Tarihi : 29 Ağustos 2019

Bibtex @araştırma makalesi { bshr598060, journal = {Journal of Biotechnology and Strategic Health Research}, issn = {}, eissn = {2587-1641}, address = {}, publisher = {Deneysel, Biyoteknolojik, Klinik ve Stratejik Sağlık Araştırmaları Derneği}, year = {2019}, volume = {3}, pages = {111 - 116}, doi = {10.34084/bshr.598060}, title = {In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells}, key = {cite}, author = {Ekşi, Saliha and Şahin Aktura, Sena and Ejder, Nebahat and Şahin, Kazım} }
APA Ekşi, S , Şahin Aktura, S , Ejder, N , Şahin, K . (2019). In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells. Journal of Biotechnology and Strategic Health Research , 3 (2) , 111-116 . DOI: 10.34084/bshr.598060
MLA Ekşi, S , Şahin Aktura, S , Ejder, N , Şahin, K . "In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells". Journal of Biotechnology and Strategic Health Research 3 (2019 ): 111-116 <https://dergipark.org.tr/tr/pub/bshr/issue/48576/598060>
Chicago Ekşi, S , Şahin Aktura, S , Ejder, N , Şahin, K . "In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells". Journal of Biotechnology and Strategic Health Research 3 (2019 ): 111-116
RIS TY - JOUR T1 - In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells AU - Saliha Ekşi , Sena Şahin Aktura , Nebahat Ejder , Kazım Şahin Y1 - 2019 PY - 2019 N1 - doi: 10.34084/bshr.598060 DO - 10.34084/bshr.598060 T2 - Journal of Biotechnology and Strategic Health Research JF - Journal JO - JOR SP - 111 EP - 116 VL - 3 IS - 2 SN - -2587-1641 M3 - doi: 10.34084/bshr.598060 UR - https://doi.org/10.34084/bshr.598060 Y2 - 2019 ER -
EndNote %0 Journal of Biotechnology and Strategic Health Research In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells %A Saliha Ekşi , Sena Şahin Aktura , Nebahat Ejder , Kazım Şahin %T In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells %D 2019 %J Journal of Biotechnology and Strategic Health Research %P -2587-1641 %V 3 %N 2 %R doi: 10.34084/bshr.598060 %U 10.34084/bshr.598060
ISNAD Ekşi, Saliha , Şahin Aktura, Sena , Ejder, Nebahat , Şahin, Kazım . "In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells". Journal of Biotechnology and Strategic Health Research 3 / 2 (Ağustos 2019): 111-116 . https://doi.org/10.34084/bshr.598060
AMA Ekşi S , Şahin Aktura S , Ejder N , Şahin K . In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells. J Biotechinol & Strategic Health Res. 2019; 3(2): 111-116.
Vancouver Ekşi S , Şahin Aktura S , Ejder N , Şahin K . In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells. Journal of Biotechnology and Strategic Health Research. 2019; 3(2): 116-111.