Araştırma Makalesi
BibTex RIS Kaynak Göster

Investigation of Babesia species using molecular methods

Yıl 2024, Cilt: 6 Sayı: 5, 335 - 340, 30.09.2024
https://doi.org/10.38053/acmj.1539570

Öz

Aims: To determine the presence of Babesia species in ticks in the Ankara region and neighboring provinces; the aim of this study was to detect and distribute Babesia species using molecular methods in ticks collected from nature and hosts.
Methods: Babesia was detected at the species level by conventional PCR method by DNA extraction of 191 tick samples collected from Ankara and neighboring provinces, which applied to the Parasitology National Reference Laboratory of the Ministry of Health, General Directorate of Public Health, between 2020-2021.
Results: A total of 191 tick samples were examined in the study, and as a result of the conventional PCR study, 4 Babesia agents were found positive, 1 of them was Babesia bigemina (B. bigemina), 2 were Babesia ovis, 1 was Babesia spp. were determined.
Conclusion: In tick-borne diseases such as Babesiosis; it should not be forgotten that ticks continue to focus some infections in nature and serve as reservoirs for disease agents in nature, and the necessary strategies for tick control should be followed.

Etik Beyan

This study was conducted with the decision of Çankırı University Ethics Committee, numbered 09.11.2021/23. The study was also conducted. In addition, permission was obtained from the head of the Microbiology Reference Laboratories and Biological Products Department for the study.

Destekleyen Kurum

non

Teşekkür

The authors would like to thank their lab colleagues for their help.

Kaynakça

  • Martínez-García G, Santamaría-Espinosa RM, Lira-Amaya JJ, et al. Challenges in tick-borne pathogen detection: the case for Babesia spp. identification in the tick vector. Pathogens 2021;10(2): 92. doi: 10.3390/pathogens10020092
  • Friedhoff, Karl T. “Transmission of babesia.” Babesiosis of domestic animals and man. CRC Press, 2018: 23-52.
  • Altay K, Dumanli N, Aktas M. Molecular identification, genetic diversity and distribution of Theileria and Babesia species infecting small ruminants. Vet Parasitol. 2007;147(1-2);161-165.
  • Çiçek H, Tandoğan M, Eser M, et al. Current status of ruminant theileriosis and its economical impact in Turkey. Türkiye Parasitol Derg. 2009;33(4):273-279.
  • Hacılarlıoğlu S, Karagenç T. Sheep Babesiosis. Animal Health Prod and Hyg. 2015;4(1):373-379.
  • Aktas M, Altay K, Dumanli N. Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction. Parasitol Res. 2007;100:797-802. doi: 10.1007/s00436-006-0345-2
  • Göksu A. Investigation of the prevalence of Babesia and Theileria Species in sheep and goats in Afyonkarahisar province (Doctoral thesis). Turkey, Afyon: Kocatepe University, Institute of Health Sciences. 2022.
  • Aktas M, Altay K, Dumanli N. Development of a polymerase chain reaction method for diagnosis of Babesia ovis infection in sheep and goats. Vet Parasitol. 2005;133:277-281. doi: 10.1016/j.vetpar.2005.05.057
  • Salas DR, Mira A, Camiida J, et al. Molecular and serological detection of Babesia bovis and Babesia bigemina infection in bovines and water buffaloes raised jointly in an endemic field. Vet Parasitol 2016; 217:101-7. doi: 10.1016/j.vetpar.2015.12.030
  • Aydın MF, Aktaş M, Dumanlı N. Molecular identification of Theileria and Babesia in sheep and goats in the Black Sea Region in Turkey. Parasitol Res. 2013;112:2817-2824. doi: 10.1007/s00436-013-3452-x
  • Estrada-Peña A, Bouattour A, Camicas JL, et al. Ticks of domestic animals in the Mediterranean Region. A guide to identification of species. Zaragoza: University of Zaragoza Press. 2004
  • Casati S, Sager H, Gern L, et al. Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland. Ann Agric Environ Med. 2006; 13:65–70.
  • Tamura K, Strecher G, Peterson D, et al. MEGA6: Molecular evolutionary genetics analysis Version 6.0. Mol Biol Evol. 2013;30(12):2725-2729. doi: 10.1093/molbev/mst197
  • Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-425. doi: 10.1093/oxfordjournals.molbev.a040454
  • Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16(2):111-120. doi: 10.1007/BF01731581
  • Felsenstien J. Confıdence lımıts on phylogenıes: an approach usıng the bootstrap. Evolution. 1985;39(4):783-791. doi: 10.1111/j.1558-5646.1985.tb00420.x
  • Kurt EK, Kandemir B, Handemir E, et al. A rare zoonotic infection in Konya; babesiosis case report. Gen Med Jl. 2021; 31(2):182-184.
  • Vannier EG, Wasser MAD, Mamoun CB, et al. Babesiosis, Infect Dis Clin North Am. 2015; 29(2):357-370. doi: 10.1016/j.idc.2015.02.008
  • Murray PR. Manual of clinical microbiology, Washington, D.C. 1970. p.2205-2209.
  • Kalkan K, Ozcelik S, Malatyali E. Investigation of Babesiosis seroprevalence in humans in Sivas. Cumhuriyet Med J. 2010; 32:276-280.
  • Karasartova DJ, Gureser AS, Tuncay Gokce T, et al. Bacterial and protozoal pathogens found in ticks collected from humans in Corum province, Turkey. PLoS Negl Trop Dis. 2018; 12(4):e0006395. doi: 10.1371/journal.pntd.0006395
  • Orkun Ö, Karaer Z, Çakmak A, et al. Determination of tick-borne pathogens in ticks feeding on humans in Turkey. PLoS Negl Trop Dis. 2014;8(8):e3067. doi: 10.1371/journal.pntd.0003067
  • Aktas M, Altay K, Dumanli N. Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction. Parasitol Res. 2007;100(4):797-802. doi: 10.1007/s00436-006-0345-2
  • Bilgiç HB, Bakırcı S, Kose O, et al. Prevalence of tick-borne haemoparasites in small ruminants in Turkey and diagnostic sensitivity of single-PCR and RLB. Parasit Vectors. 2017;10:211.
  • Gökçe T. Investigation of hard ticks (Acarı: Ixodıdae) species parasitizing on humans in Çorum region and investigation of the presence of Babesia in these ticks (Master’s Thesis) Turkey; Çorum: Hitit University, Institute of Science, 2017.
  • Zhou M, Cao S, Sevinc F, et al. Molecular detection and genetic identification of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in Turkey. Ticks Tick Borne Dis. 2016;7(1):126-134. doi: 10.1016/j.ttbdis.2015.09.008
  • Özübek S, Bastos RG, Alzan HF, et al. Bovine Babesiosis in Turkey: ımpact, current gaps, and opportunities for intervention. Pathogens. 2020;9(12):1041. doi: 10.3390/pathogens9121041
  • Ceylan O, Byamukama B, Ceylan C, et al. A survey on equine tick-borne diseases: the molecular detection of Babesia ovis DNA in Turkish racehorses, Ticks Tick Borne Dis. 2021; 12(5):101784. doi: 10.1016/j.ttbdis.2021.101784 20
  • İça A, Vatansever Z, Yıldırım A, et al. Detection of Theileria and Babesia species in ticks collected from cattle. Vet Parasitol. 2007;148:156-160. DOI: 10.1016/j.vetpar.2007.06.003
  • Köseoğlu AE, Can H, Güvendi M, et al. Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey. Parasit Vectors. 2020;14(1): 270. doi: 10.1186/s13071-021-04779-2
  • Orkun Ö, Karaer Z, Çakmak A, et al. Determination of tick-borne pathogens in ticks feeding on humans in Turkey. PLoS Negl Trop Dis. 2014;8(8):e3067. doi: 10.1371/journal.pntd.0003067
  • Orkun Ö, Karaer Z. Molecular characterization of Babesia species and ticks in wild animals in Turkey. Infect Genet Evol. 2017;55:8-13. doi: 10.1016/j.meegid.2017.08.026
  • Orkun Ö, Çakmak A, Nalbantoğlu S, et al. Turkey tick news: a molecular investigation on the presence of tick-borne pathogens in host-seeking ticks in Anatolia; first evidence of putative vectors and pathogens and footsteps of a stealthy emerging vector tick, Haemaphysalis parva. Ticks Tick Borne Dis. 2020;11(3):101373. doi: 10.1016/j.ttbdis.2020
  • Onyiche TE, Răileanu C, Fischer S, et al. Global distribution of Babesia species in questing ticks: a systematic review and meta-analysis based on published literature. Pathogens. 2021;10(2): 230. doi: 10.3390/pathogens10020230
  • Kumar B, Maharana BR, Thakre B, et al. 18S rRNA gene-based piroplasmid PCR: an assay for rapid and precise molecular screening of Theileria and Babesia species in animals. Acta Parasitol. 2022;67(4):1697-1707. doi: 10.1007/s11686-022-00625-2
Yıl 2024, Cilt: 6 Sayı: 5, 335 - 340, 30.09.2024
https://doi.org/10.38053/acmj.1539570

Öz

Kaynakça

  • Martínez-García G, Santamaría-Espinosa RM, Lira-Amaya JJ, et al. Challenges in tick-borne pathogen detection: the case for Babesia spp. identification in the tick vector. Pathogens 2021;10(2): 92. doi: 10.3390/pathogens10020092
  • Friedhoff, Karl T. “Transmission of babesia.” Babesiosis of domestic animals and man. CRC Press, 2018: 23-52.
  • Altay K, Dumanli N, Aktas M. Molecular identification, genetic diversity and distribution of Theileria and Babesia species infecting small ruminants. Vet Parasitol. 2007;147(1-2);161-165.
  • Çiçek H, Tandoğan M, Eser M, et al. Current status of ruminant theileriosis and its economical impact in Turkey. Türkiye Parasitol Derg. 2009;33(4):273-279.
  • Hacılarlıoğlu S, Karagenç T. Sheep Babesiosis. Animal Health Prod and Hyg. 2015;4(1):373-379.
  • Aktas M, Altay K, Dumanli N. Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction. Parasitol Res. 2007;100:797-802. doi: 10.1007/s00436-006-0345-2
  • Göksu A. Investigation of the prevalence of Babesia and Theileria Species in sheep and goats in Afyonkarahisar province (Doctoral thesis). Turkey, Afyon: Kocatepe University, Institute of Health Sciences. 2022.
  • Aktas M, Altay K, Dumanli N. Development of a polymerase chain reaction method for diagnosis of Babesia ovis infection in sheep and goats. Vet Parasitol. 2005;133:277-281. doi: 10.1016/j.vetpar.2005.05.057
  • Salas DR, Mira A, Camiida J, et al. Molecular and serological detection of Babesia bovis and Babesia bigemina infection in bovines and water buffaloes raised jointly in an endemic field. Vet Parasitol 2016; 217:101-7. doi: 10.1016/j.vetpar.2015.12.030
  • Aydın MF, Aktaş M, Dumanlı N. Molecular identification of Theileria and Babesia in sheep and goats in the Black Sea Region in Turkey. Parasitol Res. 2013;112:2817-2824. doi: 10.1007/s00436-013-3452-x
  • Estrada-Peña A, Bouattour A, Camicas JL, et al. Ticks of domestic animals in the Mediterranean Region. A guide to identification of species. Zaragoza: University of Zaragoza Press. 2004
  • Casati S, Sager H, Gern L, et al. Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland. Ann Agric Environ Med. 2006; 13:65–70.
  • Tamura K, Strecher G, Peterson D, et al. MEGA6: Molecular evolutionary genetics analysis Version 6.0. Mol Biol Evol. 2013;30(12):2725-2729. doi: 10.1093/molbev/mst197
  • Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-425. doi: 10.1093/oxfordjournals.molbev.a040454
  • Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16(2):111-120. doi: 10.1007/BF01731581
  • Felsenstien J. Confıdence lımıts on phylogenıes: an approach usıng the bootstrap. Evolution. 1985;39(4):783-791. doi: 10.1111/j.1558-5646.1985.tb00420.x
  • Kurt EK, Kandemir B, Handemir E, et al. A rare zoonotic infection in Konya; babesiosis case report. Gen Med Jl. 2021; 31(2):182-184.
  • Vannier EG, Wasser MAD, Mamoun CB, et al. Babesiosis, Infect Dis Clin North Am. 2015; 29(2):357-370. doi: 10.1016/j.idc.2015.02.008
  • Murray PR. Manual of clinical microbiology, Washington, D.C. 1970. p.2205-2209.
  • Kalkan K, Ozcelik S, Malatyali E. Investigation of Babesiosis seroprevalence in humans in Sivas. Cumhuriyet Med J. 2010; 32:276-280.
  • Karasartova DJ, Gureser AS, Tuncay Gokce T, et al. Bacterial and protozoal pathogens found in ticks collected from humans in Corum province, Turkey. PLoS Negl Trop Dis. 2018; 12(4):e0006395. doi: 10.1371/journal.pntd.0006395
  • Orkun Ö, Karaer Z, Çakmak A, et al. Determination of tick-borne pathogens in ticks feeding on humans in Turkey. PLoS Negl Trop Dis. 2014;8(8):e3067. doi: 10.1371/journal.pntd.0003067
  • Aktas M, Altay K, Dumanli N. Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction. Parasitol Res. 2007;100(4):797-802. doi: 10.1007/s00436-006-0345-2
  • Bilgiç HB, Bakırcı S, Kose O, et al. Prevalence of tick-borne haemoparasites in small ruminants in Turkey and diagnostic sensitivity of single-PCR and RLB. Parasit Vectors. 2017;10:211.
  • Gökçe T. Investigation of hard ticks (Acarı: Ixodıdae) species parasitizing on humans in Çorum region and investigation of the presence of Babesia in these ticks (Master’s Thesis) Turkey; Çorum: Hitit University, Institute of Science, 2017.
  • Zhou M, Cao S, Sevinc F, et al. Molecular detection and genetic identification of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in Turkey. Ticks Tick Borne Dis. 2016;7(1):126-134. doi: 10.1016/j.ttbdis.2015.09.008
  • Özübek S, Bastos RG, Alzan HF, et al. Bovine Babesiosis in Turkey: ımpact, current gaps, and opportunities for intervention. Pathogens. 2020;9(12):1041. doi: 10.3390/pathogens9121041
  • Ceylan O, Byamukama B, Ceylan C, et al. A survey on equine tick-borne diseases: the molecular detection of Babesia ovis DNA in Turkish racehorses, Ticks Tick Borne Dis. 2021; 12(5):101784. doi: 10.1016/j.ttbdis.2021.101784 20
  • İça A, Vatansever Z, Yıldırım A, et al. Detection of Theileria and Babesia species in ticks collected from cattle. Vet Parasitol. 2007;148:156-160. DOI: 10.1016/j.vetpar.2007.06.003
  • Köseoğlu AE, Can H, Güvendi M, et al. Molecular investigation of bacterial and protozoal pathogens in ticks collected from different hosts in Turkey. Parasit Vectors. 2020;14(1): 270. doi: 10.1186/s13071-021-04779-2
  • Orkun Ö, Karaer Z, Çakmak A, et al. Determination of tick-borne pathogens in ticks feeding on humans in Turkey. PLoS Negl Trop Dis. 2014;8(8):e3067. doi: 10.1371/journal.pntd.0003067
  • Orkun Ö, Karaer Z. Molecular characterization of Babesia species and ticks in wild animals in Turkey. Infect Genet Evol. 2017;55:8-13. doi: 10.1016/j.meegid.2017.08.026
  • Orkun Ö, Çakmak A, Nalbantoğlu S, et al. Turkey tick news: a molecular investigation on the presence of tick-borne pathogens in host-seeking ticks in Anatolia; first evidence of putative vectors and pathogens and footsteps of a stealthy emerging vector tick, Haemaphysalis parva. Ticks Tick Borne Dis. 2020;11(3):101373. doi: 10.1016/j.ttbdis.2020
  • Onyiche TE, Răileanu C, Fischer S, et al. Global distribution of Babesia species in questing ticks: a systematic review and meta-analysis based on published literature. Pathogens. 2021;10(2): 230. doi: 10.3390/pathogens10020230
  • Kumar B, Maharana BR, Thakre B, et al. 18S rRNA gene-based piroplasmid PCR: an assay for rapid and precise molecular screening of Theileria and Babesia species in animals. Acta Parasitol. 2022;67(4):1697-1707. doi: 10.1007/s11686-022-00625-2
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Tek Sağlık
Bölüm Research Articles
Yazarlar

Sinem Tunçer Bu kişi benim 0000-0002-3200-5178

Banuçiçek Yücesan 0000-0001-7051-3045

Yayımlanma Tarihi 30 Eylül 2024
Gönderilme Tarihi 27 Ağustos 2024
Kabul Tarihi 22 Eylül 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 6 Sayı: 5

Kaynak Göster

AMA Tunçer S, Yücesan B. Investigation of Babesia species using molecular methods. Anatolian Curr Med J / ACMJ / acmj. Eylül 2024;6(5):335-340. doi:10.38053/acmj.1539570

Üniversitelerarası Kurul (ÜAK) Eşdeğerliği: Ulakbim TR Dizin'de olan dergilerde yayımlanan makale [10 PUAN] ve 1a, b, c hariç uluslararası indekslerde (1d) olan dergilerde yayımlanan makale [5 PUAN]

-  Dahil olduğumuz İndeksler (Dizinler) ve Platformlar sayfanın en altındadır.

Not: Dergimiz WOS indeksli değildir ve bu nedenle Q olarak sınıflandırılmamaktadır.

Yüksek Öğretim Kurumu (YÖK) kriterlerine göre yağmacı/şüpheli dergiler hakkındaki kararları ile yazar aydınlatma metni ve dergi ücretlendirme politikasını tarayıcınızdan indirebilirsiniz. https://dergipark.org.tr/tr/journal/3449/page/10809/update 

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