Mitochondrial Origins of Late Byzantine Burials in Kadıkalesi

Öz

Objective: In the presented study, it was aimed to examine aDNA from human biological materials between the 13th and 15th centuries, obtained from the Kadıkalesi Anaia excavations, and to determine their maternal origins from Mitochondrial DNA. In this way, it was aimed to determine the mitochondrial haplogroups of the region. Methods: For this purpose; The remains of 20 individuals from the archeological excavations in 2016-2018 and dated to the Byzantine period were examined. Anthropometric examinations were first performed on individuals, and then Sanger sequencing of mitochondial HVR1 and HVR2 regions were examined. The obtained sequencing data were evaluated by online programs. Results: According to the result of the study, 12 out of 20 individuals were identified in terms of age, height and gender. As a result of the examination, the maternal haplogroup of 16 individuals was reached and it was found that the majority of them consisted of West Eurasian macrohaplogroups. In addition, two individuals with sub-Saharan haplogroups were identified. Post mortem DNA damage was observed in most of the group . Conclusion: In the present study, genetic information was obtained about the structure of the late Byzantine society in Kadıkalesi Anaia. These results will provide very important information to learn the genetics of the social structure of the period in other excavation areas to be made in the region.

Anahtar Kelimeler

• Anaia excavation
• Genetics
• Ancient DNA
• mitochondria

Kadıkalesi Geç Bizans Dönemi Gömülerinin Mitokondriyel Kökenleri

Öz

Amaç: Sunulan çalışmada, Kadıkalesi Anaia kazılarından elde edilen, 13.- 15. yüzyıllar arasındaki insana ait biyolojik materyallerde aDNA elde edilip, Mitokondriyel DNA’dan maternal kökenlerinin tayin edilmesi planlanmıştır. Bu sayede bölgenin mitokondriyel haplogruplarının tespiti hedeflenmiştir. Yöntem: Bu amaçla; 2016-2018 yıllarındaki açmalardan elde edilen ve Bizans dönemine tarihlendirilen 20 bireye ait kalıntılar incelenmiştir. Bireylerde önce antropometrik incelemeler yapılmış, ardından Sanger dizileme ile mitokondriyel HVR1 ve HVR2 bölgeleri incelendi. Elde edilen dizileme verileri online programlar vasıtası ile değerlendirildi. Bulgular: Çalışmanın sonucunda 20 bireyden 12’si yaş, boy ve cinsiyet bakımından kimliklendirilmiştir. İnceleme sonucu 16 bireyin maternal haplogrubuna erişilmiş ve çoğunluğunun Batı Avrasya makrohaplogruplarından oluşmuş olduğu bulunmuştur. Buna ek olarak Sahra-altı haplogruplara sahip iki birey saptanmıştır. Grubun çoğunda post mortem DNA hasarı gözlemlenmiştir. Sonuç: Sunulan çalışmada Kadıkalesi Anaia’daki geç bizans dönemi toplumunun yapısı hakkında hakkında genetik bilgi elde edilmiştir. Bu sonuçlar bölgede yapılacak diğer kazı alanlarında, dönemin toplum yapısının genetiğini öğrenmek için oldukça önemli bilgiler sağlayacaktır.

Anahtar Kelimeler

• Anaia kazıları
• antik DNA
• Genetik
• mitokondri

Kaynakça

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