EARLY AND LATE STAGE DEPENDENT ANTICANCER EFFECTS OF Laurus nobilis SEED EXTRACTS ON PC3 PROSTATE CANCER CELLS

Öz

Objective: This study explored the anticancer properties of Laurus nobilis seed extracts at different maturity stages on PC3 prostate cancer cells, aiming to understand how developmental stages influence biological activities. Methods: Extracts from early-stage (green) and late-stage (black) L. nobilis seeds were tested on PC3 cells. Cytotoxicity, cell migration, wound healing, and gene expression were analyzed. HUVEC cells were used to assess toxicity on normal cells. Results: Early-stage extracts showed low cytotoxicity and promoted cell migration and wound healing. Late-stage extracts exhibited potent dose-dependent cytotoxicity and significantly inhibited cell migration. Both extracts demonstrated lower toxicity towards HUVEC cells. Gene expression analysis revealed suppression of epithelial-mesenchymal transition and migration in cells treated with late-stage extracts. Conclusion: The study highlights the stage-dependent anticancer potential of L. nobilis seed extracts. Late-stage extracts showed promising anticancer effects, while early-stage extracts promoted cell growth. These findings emphasize the importance of harvest time and maturity stage in determining the biological activity of L. nobilis extracts, providing valuable insights for future anticancer research and applications.

Anahtar Kelimeler

• Laurus nobilis
• Prostate cancer
• Seed extracts
• Stage-dependent effects
• Anticancer activity

Laurus nobilis TOHUM EKSTRELERİNİN PC3 PROSTAT KANSERİ HÜCRELERİ ÜZERİNDEKİ ERKEN VE GEÇ EVRE BAĞIMLI ANTİKANSER ETKİLERİ

Öz

Amaç: Bu çalışma, gelişim aşamalarının biyolojik aktiviteleri nasıl etkilediğini anlamak amacıyla, farklı olgunluk aşamalarındaki Laurus nobilis tohum özütlerinin PC3 prostat kanseri hücreleri üzerindeki antikanser özelliklerini araştırmıştır. Yöntem: Erken aşama (yeşil) ve geç aşama (siyah) L. nobilis tohumlarından elde edilen özütler PC3 hücreleri üzerinde test edilmiştir. Sitotoksisite, hücre göçü, yara iyileşmesi ve gen ekspresyonu analiz edilmiştir. Normal hücreler üzerindeki toksisiteyi değerlendirmek için HUVEC hücreleri kullanılmıştır Bulgular: Erken aşama özütleri düşük sitotoksisite göstermiş ve hücre göçünü ve yara iyileşmesini teşvik etmiştir. Geç aşama özütleri, güçlü doz bağımlı sitotoksisite göstermiş ve hücre göçünü önemli ölçüde inhibe etmiştir. Her iki özüt de HUVEC hücrelerine karşı daha düşük toksisite göstermiştir. Gen ekspresyonu analizi, geç aşama özütleri ile tedavi edilen hücrelerde epitelyal-mezenkimal geçişin ve göçün baskılanmasını ortaya koymuştur. Sonuç: Çalışma, L. nobilis tohum özütlerinin aşama bağımlı antikanser potansiyelini vurgulamaktadır. Geç aşama özütleri umut verici antikanser etkileri gösterirken, erken aşama özütleri hücre büyümesini teşvik etti. Bu bulgular, L. nobilis özütlerinin biyolojik aktivitesinin belirlenmesinde hasat zamanı ve olgunluk aşamasının önemini vurgulamakta ve gelecekteki antikanser araştırmaları ve uygulamaları için değerli bilgiler sağlamaktadır.

Anahtar Kelimeler

• Laurus nobilis
• Prostate cancer
• Seed extracts
• Stage-dependent effects
• Anticancer activity

Kaynakça

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