Bazı Bakteriyel Balık Patojenlerinde Biyofilm Oluşumunun Farklı İn Vitro Metodlarla Tespiti

Öz

Bu çalışmada, fenomen bir virülens faktörü olan biyofilmin bazı bakteriyel balık patojenlerinden Staphylococcus warneri, Aeromonas sobria, Yersinia ruckeri, Flavobacterium psychrophilum, Vibrio anguillarum bakterileri tarafından üretilmesi tüp, mikroplak ve kongo red agar yöntemleriyle araştırılmıştır.

Tüp yöntemi ile biyofilm oluşum dereceleri tüpün iç yüzeyinde oluşan film tabakasının kalınlığına göre sırayla F. psychrophilum’da (++), A. sobria’da (++), S. warneri’de (+), Y. ruckeri’de (-), V. anguillarum’da (-) olarak tespit edilmiştir. Mikroplaklarla yapılan biyofilm testinde plaklarda oluşan biyofilm negatif kontrolde 0,048, S. warneri’de 0,121, A. sobria’da 0,158, Y. ruckeri’de 0,071, F. psychrophilum’da 0,172, V. anguillarum’da 0,212 absorbans değeri tespit edilmiştir. Mikroplak testinde biyofilm oluşumu sırayla V. anguillarum, F. psychrophilum, A. sobria, S. warneri, Y. ruckeri olarak belirlenmiştir. Kongo red agar testinde biyofilm (slime) oluşturan bakterilerin koloni morfolojisinin renk değiştirmesine göre biyofilm oluşumu sırayla V. anguillarum, A. sobria, S. warneri, F. psychrophilum, Y. ruckeri olarak belirlenmiştir. Kongo red agarda kuru kristalize siyah koloni üreterek en güçlü biyofilm oluşumu V. anguillarum, A. sobria, S. warneri’de bulunmuştur. Pembe renkli koloni üreten F. psychrophilum ve Y. ruckeri bakterileri ise bu teste biyofilm oluşumu göstermemiştir.

     Sonuç olarak; biyofilm kaynaklı enfeksiyonlar balıklar üzerinde ölümcül etki göstermektedir. Bu etkilerin araştırılması ve durdurulması balık ölümlerinin azaltılmasında önemlidir. Bakteriyel balık patojenlerinden olan S. warneri, A. sobria, V. anguillarum, F. psychrophilum, Y. ruckeri’nin araştırmamızda oldukça güçlü biyofilm oluşturdukları farklı yöntemlerle tespit edilmiştir. 

Anahtar Kelimeler

• Bakteriyel balık patojenleri,biyofilm,tüp yöntemi,mikroplak yöntemi,kongo red agar yöntem

Kaynakça

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