Diagnosis of Photobacterium sanguinicancri in Smout-hound Shark (Mustelus mustelus, Linnaeus 1758)

Öz

This study aims to determine the cause of mortalities in smooth-hound sharks (Mustelus mustelus, Linnaeus 1758) under stressful conditions from a marine aquarium in Turkey. Moribund smooth-hound sharks externally had haemorrhages on various body parts, especially the pectoral fins and certain areas of their skin. A pale liver and spleen pseudo-tubercles were the main pathological findings internally. For bacteriological examination, samples were taken from visceral organs and blood and streaked onto marine agar (MA) plates which were incubated at 22°C for 72 hours. Gram-negative motile rods that displayed a bipolar staining were isolated from several tissues, including the spleen, kidney and liver. According to their morphological and biochemical characteristics as well as 16S rRNA gene sequencing, these isolates were identified as Photobacterium sanguinicancri. Therefore, it was concluded that photobacteriosis was responsible for this disease outbreak in aquarium-kept smooth-hound sharks. According to the literature survey this is the first report on describing photobacteriosis caused by Photobacterium sanguinicancri in this shark species.

Anahtar Kelimeler

• Smooth-hound Shark
• Photobacteriosis
• Photobacterium sanguinicancri
• Aquarium

Tazı Köpekbalıklarında (Mustelus mustelus, Linnaeus 1758) Photobacterium sanguinicancri'nin Tanısı

Öz

Bu çalışma, Türkiye'deki bir deniz akvaryumunda stresli koşullar altında bulunan tazı köpekbalıklarında (Mustelus mustelus, Linnaeus 1758) meydana ölümlerin nedenlerinin belirlenmesini amaçlamaktadır. Eksternal olarak ölmek üzere olan tazı köpekbalıklarının çeşitli vücut kısımlarında, özellikle göğüs yüzgeçlerinde ve deride belirli bölgelerinde kanamaları vardı. İnternal olarak soluk karaciğer ve dalakta psödotüberküller ana patolojik bulgulardı. Bakteriyolojik inceleme için iç organlardan ve kandan örnekler marine agar (MA) içeren petrilere ekimler yapılmış ve 22°C'de 72 saat inkübe edilmiştir. Bipolar boyama özelliği gösteren gram negatif hareketli basiller dalak, böbrek ve karaciğer dahil olmak üzere çeşitli dokulardan izole edilmiştir. Morfolojik ve biyokimyasal özelliklerine ve 16S rRNA gen dizilimine göre, bu izolatlar Photobacterium sanguinicancri olarak tanımlandı. Bu nedenle, akvaryumda tutulan tazı köpekbalıklarında bu hastalık salgınına photobacteriosis’in neden olduğu sonucuna varılmıştır. Literatür araştırmasına göre, bu köpekbalığı türünde Photobacterium sanguinicancri'nin neden olduğu photobacteriosis'i tanımlayan ilk rapor budur.

Anahtar Kelimeler

• Photobacresiosis
• Photobacterium sanguinicancri
• Akvaryum
• Tazı köpekbalığı

Kaynakça

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