Marmara Denizi’nde Yayılış Gösteren Aurelia aurita Denizanası Türünün Filogenetik Analizi
Yıl 2019,
Cilt: 15 Sayı: 2, 163 - 170, 14.06.2019
Meltem Güneş
Fikriye Polat
Öz
Çalışmamızda Marmara Denizi sahillerinde
yayılış gösteren Knidliler şubesine ait Aurelia
aurita denizanası türü filogenetik olarak incelenmiştir. Bunun için Ekim
2017- Şubat 2018 tarihleri arasında Marmara Denizi’nin kuzey ve güney
sahillerinden toplanan denizanası örneklerinden DNA’lar izole edilmiş ve mitokondrial
Sitokrom Oksidaz Altünite I (COI), 16S rDNA, nüklear 18S rDNA ve Internal
Transcribed Spacer 1 (ITS1) gen bölgeleri Polimeraz Zincir reaksiyonu (PCR) ile
amplifiye edilmiş ve ardından dizi analizleri yapılmıştır. Filogenetik
analizler GenBank’tan alınan dizilerle birlikte
değerlendirilerek gerçekleştirilmiş ve türler arası ilişkiler belirlenmiştir.
Kaynakça
- Appeltans, W., Ahyong, S. T., Anderson, G., Angel, M. V., Artois, T., Bailly, N., Bamber, R., Barber, A., Batsch, I., & Berta, A. (2012). The Magnitude of Global Marine Species Diversity. Current Biology, 22, 2189-2202.
- Arai, M. N. (1996). Functional Biology of Scyphozoa, 1st ed., Chapman and Hall, New York.
- Batovska, J., Cogan, N. O., Lynch, S. E., & Blacket, M. J. (2017). Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2, G3 Genes/Genomes/Genetics, 7, 19-29.
- Bayha, K. M., Dawson, M. N., Collins, A. G., Barberitos, M. S., & Haddock, S. H. D. (2010). Evolutionary relationships among scyphozoan jellyfish families based on complete taxon sampling and phylogenetic analysis of 18S and 28S ribosomal DNA, Integrative and Comparative Biology, 50 (3), 436-455.
- Beauchamp, K. A., & Powers, D. A. (1996). Sequence variation of the first ınternal spacer (ITS1-1) of Ribosomal DNA in ahermatypic corals from California, Molecular marine biology and biotechnology., 5, 357-362.
- Bucklin, A., Steinke, D., & Blanco-Bercial L. (2011). DNA barcoding of marine metazoa. Annual Reviews of Marine Science. 3, 471-508.
- Chalwatzis, N., Bauer, A., Stetzer, E., Kinzelbach, R., & Zimmermann, F. K. (1995). Strongly expanded 18S rRNA genes correlated with a peculiar morphology in the ınsect order of Strepsiptera, Zoology, 98, 115-126.
- Chen, C. A., Willis, B. L., & Miller, D. J. (1996). Systematic relationships between tropical corallimopharians (Cnidaria: Anthozoa: Corallimorpharia): utility of the 5.8S and ınternal transcribed spacer (ITS) regions of the rDNA transcription unit, Bulletin of Marine Science, 59, 196-208.
- Coll, M., Piroddi, C., Steenbeek, J., Kaschner, K., Lasram, F. B. R., Aguzzi, J., Ballesteros, E., Bianchi, C. N., Corbera, J., & Dailianis, T. (2010). The biodiversity of the Mediterranean Sea: estimates, patterns and threads, PLoS, 5,11842.
- Çardak, M. (2002). Çanakkale Boğazı, Nara Burnu civarındaki scyphozoa ve ctenophora türleri ile bunların bölgedeki dağılımlarının araştırılması, Yüksek Lisans Tezi, Çanakkale Onsekiz Mart Üniversitesi, Fen Bilimleri Enstitüsü, ref. no: 121545.
- Dawson, M. N., & Jacobs, D. K. (2001). Molecular evidence for cryptic species of Aurelia aurita (Cnidaria, Scyphozoa), The Biological Bulletin, 200, 92-96.
- Dawson, M. N. (2005).Incipient speciation of Catostylus mosaicus (Scyphozoa, Rhizostomeae, Catostylidae), comparative phylogeography and biogeography in southeastern Australia, Journal of Biogeography 32, 515-533.
- Doğan, O. (2017). Türkiye Kıyılarında Dağılım Gösteren Scyphozoa Sınıfına Ait Bazı Türlerin Moleküler Filogenisi, Yüksek Lisans Tezi, İstanbul Üniversitesi, Fen Bilimleri Enstitüsü, İstanbul, ref. no: 462931.
- Hamner, W.M., Dawson, M.N. (2009). A systematic review of the evolution of jellyfish blooms: advantageous aggregations and adaptive assemblages. Hydrobiologia, 616, 161-191.
Häussermann, V., Dawson, M.N., Försterra, G.( 2009). First record of the moon jellyfish, Aurelia for Chile. Spixiana 32(1),3-7.
- Hwang, U. W., Kim, W. (1999). General properties and phylogenetic utilities of nuclear ribosomal DNA and mitochondrial DNA commonly used in molecular systematics, The Korean Journal of Parasitology, 37 (4), 215.
- İşinibilir, M., Yilmaz, I. N., & Demirel, N. (2015). New records of jellyfish species in the Marmara Sea, Italian Journal of Zoology, 82, 425-429.
- Keskin, E., & Atar, H. H. (2013). DNA barcoding commercially ımportant aquatic invertebrates of Turkey, Mitochondrial DNA The Journal of DNA Mapping, Sequencing, and
Analysis, 24 (4), 440-450.
- Kramp, P. (1961). Sinopsis of the medusae of the world, J. Mar. Biol. Assoc., 40, 1-469.
- Miller, S. A., Harley, J. P. (2016). Zoology, 10th ed., McGraw Hill, New York, 2016.
- Odorico, D. M., & Miller, D. J. (1997). Variation in the ribosomal ınternal trandcribed spacers and 5.8S rDNA among five species of acropora (Bnidaria: Scleractinia): patterns of variation consistent with reticulate evolution, Mol. Biol. Evol., 14, 465-473.
- Ortman, B. D., Bucklin, A., Pages, F., & Youngbluth, M. (2010). DNA barcoding the Medusozoa using mtCOI. Deep-Sea Res Pt II. 57, 2148-2156.
- Özbalcılar, B. (2012). Türkiye denizlerinde bulunan Akdeniz ve İndo-Pasifik kökenli denizanaları'nın filogenetiği, Yüksek Lisans Tezi, Mustafa Kemal Üniversitesi, Fen Bilimleri Enstitüsü, Hatay, ref. no:329548.
- Özer, N. P. (1994). Rhizostoma pulmo (Macri, 1778) Denizanasının İşleme ve Değerlendirme Yöntemlerinin Karşılaştırılması, Doktora Tezi, Karadeniz Teknik Üniversitesi, Fen Bilimleri Enstitüsü, ref. no: 33737.
- Palumbi, S. R. (1996). Nucleic acids II: The polymerase chain reaction. In Molecular systematics. Edited by Hillis DM, Moritz C, Mable BK. Sinauer Associates, Inc.; 205-247.
- Rızman-Idid, M., Farrah-Azwa, A.B., & Chong, V.C. (2016). Preliminary Taxonomic Survey and Molecular Documentation of Jellyfish Species (Cnidaria: Scyphozoa and Cubozoa) in Malaysia. Zoological Studies, 55(35), 1-19 doi:10.6620/ZS.2016.55-35
- Tanyolaç, J., & Tanyolaç, T. (2000). Genel Zooloji, 6. Baskı, Hatiboğlu Yayınevi, Ankara.
- Taşer, Y. (2017). Denizanalarının farklı alanlarda değerlendirilmesi (Cnidaria: Coelenterata), Yüksek Lisans Tezi, Katip Çelebi Üniversitesi, Fen Bilimleri Enstitüsü, İzmir, ref. no: 459006.
- Wesson, D. M., Porter, C. H., & Colling, F. H. (1992). Sequence and secondary structure comparisons of ITS rDNA in mosquitoes (Diptera: Culicidae), Molecular phylogenetics and evolution, 1, 253-269.
Phylogenetic Analysis of Jellyfish Species of Aurelia aurita Distributed in the Sea of Marmara
Yıl 2019,
Cilt: 15 Sayı: 2, 163 - 170, 14.06.2019
Meltem Güneş
Fikriye Polat
Öz
In our study, the species Aurelia aurita jellyfish belonging to the Cnidaria branch distributed in the coasts of the Marmara Sea were examined phylogenetically. For this purpose, DNAs were isolated from the jellyfish samples collected from the northern and southern coasts of the Marmara Sea between October 2017 and February 2018, and mitochondrial Cytochrome Oxidase Subunit I (COI), 16S rDNA, nuclear 18S rDNA and Internal Transcribed Spacer 1 (ITS1) gene regions were amplified by PCR and sequence analysis was performed. Phylogenetic analysis was carried out with the evaluation of the obtained results together with the sequences obtained from GenBank and inter-species relationships were determined.
Kaynakça
- Appeltans, W., Ahyong, S. T., Anderson, G., Angel, M. V., Artois, T., Bailly, N., Bamber, R., Barber, A., Batsch, I., & Berta, A. (2012). The Magnitude of Global Marine Species Diversity. Current Biology, 22, 2189-2202.
- Arai, M. N. (1996). Functional Biology of Scyphozoa, 1st ed., Chapman and Hall, New York.
- Batovska, J., Cogan, N. O., Lynch, S. E., & Blacket, M. J. (2017). Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2, G3 Genes/Genomes/Genetics, 7, 19-29.
- Bayha, K. M., Dawson, M. N., Collins, A. G., Barberitos, M. S., & Haddock, S. H. D. (2010). Evolutionary relationships among scyphozoan jellyfish families based on complete taxon sampling and phylogenetic analysis of 18S and 28S ribosomal DNA, Integrative and Comparative Biology, 50 (3), 436-455.
- Beauchamp, K. A., & Powers, D. A. (1996). Sequence variation of the first ınternal spacer (ITS1-1) of Ribosomal DNA in ahermatypic corals from California, Molecular marine biology and biotechnology., 5, 357-362.
- Bucklin, A., Steinke, D., & Blanco-Bercial L. (2011). DNA barcoding of marine metazoa. Annual Reviews of Marine Science. 3, 471-508.
- Chalwatzis, N., Bauer, A., Stetzer, E., Kinzelbach, R., & Zimmermann, F. K. (1995). Strongly expanded 18S rRNA genes correlated with a peculiar morphology in the ınsect order of Strepsiptera, Zoology, 98, 115-126.
- Chen, C. A., Willis, B. L., & Miller, D. J. (1996). Systematic relationships between tropical corallimopharians (Cnidaria: Anthozoa: Corallimorpharia): utility of the 5.8S and ınternal transcribed spacer (ITS) regions of the rDNA transcription unit, Bulletin of Marine Science, 59, 196-208.
- Coll, M., Piroddi, C., Steenbeek, J., Kaschner, K., Lasram, F. B. R., Aguzzi, J., Ballesteros, E., Bianchi, C. N., Corbera, J., & Dailianis, T. (2010). The biodiversity of the Mediterranean Sea: estimates, patterns and threads, PLoS, 5,11842.
- Çardak, M. (2002). Çanakkale Boğazı, Nara Burnu civarındaki scyphozoa ve ctenophora türleri ile bunların bölgedeki dağılımlarının araştırılması, Yüksek Lisans Tezi, Çanakkale Onsekiz Mart Üniversitesi, Fen Bilimleri Enstitüsü, ref. no: 121545.
- Dawson, M. N., & Jacobs, D. K. (2001). Molecular evidence for cryptic species of Aurelia aurita (Cnidaria, Scyphozoa), The Biological Bulletin, 200, 92-96.
- Dawson, M. N. (2005).Incipient speciation of Catostylus mosaicus (Scyphozoa, Rhizostomeae, Catostylidae), comparative phylogeography and biogeography in southeastern Australia, Journal of Biogeography 32, 515-533.
- Doğan, O. (2017). Türkiye Kıyılarında Dağılım Gösteren Scyphozoa Sınıfına Ait Bazı Türlerin Moleküler Filogenisi, Yüksek Lisans Tezi, İstanbul Üniversitesi, Fen Bilimleri Enstitüsü, İstanbul, ref. no: 462931.
- Hamner, W.M., Dawson, M.N. (2009). A systematic review of the evolution of jellyfish blooms: advantageous aggregations and adaptive assemblages. Hydrobiologia, 616, 161-191.
Häussermann, V., Dawson, M.N., Försterra, G.( 2009). First record of the moon jellyfish, Aurelia for Chile. Spixiana 32(1),3-7.
- Hwang, U. W., Kim, W. (1999). General properties and phylogenetic utilities of nuclear ribosomal DNA and mitochondrial DNA commonly used in molecular systematics, The Korean Journal of Parasitology, 37 (4), 215.
- İşinibilir, M., Yilmaz, I. N., & Demirel, N. (2015). New records of jellyfish species in the Marmara Sea, Italian Journal of Zoology, 82, 425-429.
- Keskin, E., & Atar, H. H. (2013). DNA barcoding commercially ımportant aquatic invertebrates of Turkey, Mitochondrial DNA The Journal of DNA Mapping, Sequencing, and
Analysis, 24 (4), 440-450.
- Kramp, P. (1961). Sinopsis of the medusae of the world, J. Mar. Biol. Assoc., 40, 1-469.
- Miller, S. A., Harley, J. P. (2016). Zoology, 10th ed., McGraw Hill, New York, 2016.
- Odorico, D. M., & Miller, D. J. (1997). Variation in the ribosomal ınternal trandcribed spacers and 5.8S rDNA among five species of acropora (Bnidaria: Scleractinia): patterns of variation consistent with reticulate evolution, Mol. Biol. Evol., 14, 465-473.
- Ortman, B. D., Bucklin, A., Pages, F., & Youngbluth, M. (2010). DNA barcoding the Medusozoa using mtCOI. Deep-Sea Res Pt II. 57, 2148-2156.
- Özbalcılar, B. (2012). Türkiye denizlerinde bulunan Akdeniz ve İndo-Pasifik kökenli denizanaları'nın filogenetiği, Yüksek Lisans Tezi, Mustafa Kemal Üniversitesi, Fen Bilimleri Enstitüsü, Hatay, ref. no:329548.
- Özer, N. P. (1994). Rhizostoma pulmo (Macri, 1778) Denizanasının İşleme ve Değerlendirme Yöntemlerinin Karşılaştırılması, Doktora Tezi, Karadeniz Teknik Üniversitesi, Fen Bilimleri Enstitüsü, ref. no: 33737.
- Palumbi, S. R. (1996). Nucleic acids II: The polymerase chain reaction. In Molecular systematics. Edited by Hillis DM, Moritz C, Mable BK. Sinauer Associates, Inc.; 205-247.
- Rızman-Idid, M., Farrah-Azwa, A.B., & Chong, V.C. (2016). Preliminary Taxonomic Survey and Molecular Documentation of Jellyfish Species (Cnidaria: Scyphozoa and Cubozoa) in Malaysia. Zoological Studies, 55(35), 1-19 doi:10.6620/ZS.2016.55-35
- Tanyolaç, J., & Tanyolaç, T. (2000). Genel Zooloji, 6. Baskı, Hatiboğlu Yayınevi, Ankara.
- Taşer, Y. (2017). Denizanalarının farklı alanlarda değerlendirilmesi (Cnidaria: Coelenterata), Yüksek Lisans Tezi, Katip Çelebi Üniversitesi, Fen Bilimleri Enstitüsü, İzmir, ref. no: 459006.
- Wesson, D. M., Porter, C. H., & Colling, F. H. (1992). Sequence and secondary structure comparisons of ITS rDNA in mosquitoes (Diptera: Culicidae), Molecular phylogenetics and evolution, 1, 253-269.