Spirulina platensis’ten mavi pigment fikosiyanin ekstraksiyonu

Yıl 2020, Cilt: 16 Sayı: 4, 506 - 510, 01.12.2020

Oya Işık , Leyla Uslu , Burcu Ak Çimen , Sevket Gökpınar Choubaila Reddad Selin Sayın

https://doi.org/10.22392/actaquatr.732774

Öz

Fikosiyanin, Spirulina platensis’ten elde edilen ve suda çözünebilen mavi pigmenttir. Bu çalışmada fikosiyanin ekstraksiyonu için çözücü olarak su kullanılmıştır. Fikosiyanin konsantrasyonunu (PC) (mg mL-1) belirlemek için yaş Spirulina biyoması, oda sıcaklığında 48 saat beklemiş yaş biyomas, 48 saat dondurulmuş Spirulina, 15 gün dondurulmuş Spirulina ve kuru Spirulina kullanılmıştır. Aynı zamanda Fikosiyanin saflığı (OD615/OD280) ve ürün miktarı (mg g-1) saptanmıştır. Onbeşgün dondurulmuş Spirulina,48 saat oda sıcaklığında beklemiş yaş biyomas ve yaş Spirulina biyomasından, sırasıyla 17,6497, 17,1370 ve 17,0833 mg g-1fikosiyanin elde edilmiştir. Üründeki saflık oranları (OD615/OD280), 4.60, 0.81, 0.85, 0.98 ve 6.55, sırasıyla onbeşgün dondurulmuş Spirulina, 48 saat dondurulmuş Spirulina, 48 saat bekletilmiş yaş Spirulina biyoması, kurutulmuş Spirulina ve yaş biyomastan elde edilmiştir.

Anahtar Kelimeler

Fikosiyanin, ekstraksiyon, çözücü, C-PC ürün

Blue colored pigment phycocyanin extraction from Spirulina platensis

Öz

Phycocyanin is a blue pigment and water-soluble biliprotein from the Spirulina platensis. In this study, the water was used as a solvent for the extraction of phycocyanin. Wet biomass of Spirulina, wet biomass held for 48 hours at room temperature, frozen Spirulina for 48 hours, and frozen biomass for fifteen days, and dried Spirulina was used to determine phycocyanin concentration (PC) (mg mL-1). At the same time, extract purity of phycocyanin (OD615/OD280) and yield (mg g-1) were determined. Phycocyanin yield of 17,6497, 17,1370, and 17,0833 mg g-1 were obtained from frozen Spirulina for fifteen days, wet biomass of Spirulina held for 48 hours, and wet biomass of Spirulina, respectively. The purity ratios (OD615/OD280) of 4.60, 0.81, 0.85, 0.98, and 6.55 were determined for frozen for 15 days, frozen Spirulina for 48 hours, wet biomass of Spirulina (waited for 48 hours), dried Spirulina and wet Spirulina, respectively

Anahtar Kelimeler

Phycocyanin, extraction, solvent, C-PC yield

Kaynakça

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