Çinkonun Güney Tıbbi Sülüğü Hirudo verbana Carena, 1820 Üzerine Akut Toksisitesi

Yıl 2021, Cilt: 17 Sayı: 3, 421 - 428, 01.09.2021

Mustafa Ceylan , Osman Çetinkaya , Cafer Bulut

https://doi.org/10.22392/actaquatr.874241

Cited By: 2

Öz

Bu çalışma ile çinkonun ekotoksikolojik çalışmalarda kullanılmakta olan güney tıbbi sülüğü Hirudo verbana Carena, 1820 üzerine akut toksisitesinin belirlenmesi amaçlanmıştır. Deneme 96 saat süreli yarı statik biyodeney yöntemiyle iki tekerrürlü yürütülmüştür. Bu amaçla, sülükler 6,25 mg/L, 12,50 mg/L, 25 mg/L, 50 mg/L, 100 mg/L ve 200 mg/L’lik çinko konsantrasyonlarına maruz bırakılmıştır. Deney ve hesaplamalar sonucunda LC50 değerleri 24, 48, 72 ve 96. saatler için sırasıyla 48,30 mg/L, 19,68 mg/L, 15,48 mg/L ve 14,12 mg/L olarak belirlenmiştir. 25 mg/L, 50 mg/L, 100 mg/L ve 200 mg/L konsantrasyonları için belirlenen LT50 değerleri sırasıyla 38,63 saat, 24,55 saat, 14,62 saat ve 12,78 saat olarak hesaplanmıştır. Çinkoya maruz kalmış sülüklerde dışkılama, aşırı mukus çıkışı, kontrolsüz yüzme, ortamdan uzaklaşma girişimleri, tutunma gücünün zayıflaması, kıvrılma (burkulma), sindirilmiş kanın kusulması, vücutta oluşan deformasyonlar, erkeklik organının dışa sarkması, hemoraji ve vücut duvarında aşınmalar başlıca fizyolojik ve davranışsal tepkileri oluşturmuştur. Elde edilen veriler, diğer organizmalarla kıyaslandığında H. verbana türünün çinko duyarlılığının orta düzeyde olduğunu ortaya koymuştur. Bu çalışmada elde edilen verilerin nesli tehlike altında olan tıbbi sülük popülasyonlarının yönetimine katkı sağlaması beklenmektedir.

Anahtar Kelimeler

ağır metal, biyodeney, mortalite, LC50, LT50

Acute Toxicity of Zinc on Southern Medicinal Leech, Hirudo verbana Carena, 1820

Öz

In the present study it was aimed to determine the acute toxicity of zinc on southern medicinal leech Hirudo verbana Carena, 1820 that being used in the ecotoxicological studies. The experiment was conducted for 96 hours using the semi-static bioassay method with replicate two times. The leeches were exposed to zinc concentrations of 6.25 mg/L, 12.50 mg/L, 25 mg/L, 50 mg/L, 100 mg/L and 200 mg/L, respectively. As a result of the experiment, the LC50 values for 24, 48, 72 and 96 hours were determined 48.30 mg/L, 19.68 mg/L, 15.48 mg/L, and 14.12 mg/L, respectively. The LT50 values for 25 mg/L, 50 mg/L, 100 mg/L and 200 mg/L zinc concentrations were determined 38.63 hours, 24.55 hours, 14.62 hours, and 12.78 hours, respectively. Defecation, intensive mucus output, uncontrolled swimming, attempt to escape from the experiment media, reducing the cling power, coiling (buckling), vomiting the digestive blood, the body deformations, penile prolapse, hemorrhage and abrasion in the wall of the body were the main physiological and behavioral responses in the leeches which were exposed to the different zinc solutions. The data obtained revealed that the zinc sensitivity of H. verbana was moderate when compared to other organisms. It is expected that the data of the present study will contribute to the management of endangered medicinal leech populations.

Anahtar Kelimeler

heavy metal, bioassay, mortality, LC50, LT50

Kaynakça

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