Asker Sineği Hermetia illucens ve Un Kurdu Tenebrio molitor Larvaları İle Beslenen Pontastacus leptodactylus Ergenlerinin Büyüme Performansı ve Yaşama Oranı

Yıl 2024, Cilt: 20 Sayı: 4, 343 - 350

Seval Bahadır Koca , Habil Uğur Koca , Hasan Batuhan Emre Özdoğan , Nalan Özgür Yigit

https://doi.org/10.22392/actaquatr.1452851

Öz

Bu çalışmada Pontastacus leptodactylus juvenile kerevitinin beş farklı yemle beslenmesi durumunda yaşama oranı ve büyüme performansı değerlendirilmiştir. Deneme grupları; CF: ticari yem, HI: Taze Hermetia illucens, CF+Hİ; %50 ticari yem + %50 taze Hermetia illucens, TM: Taze Tenebrio molitor, CF+TM: %50 ticari yem + %50 taze Tenebrio molitor. Denemeler 60 gün sürmüştür. Sonuçlar yaşam oranı, nihai vücut ağırlığı ve ağırlık kazancının taze BSF ve TM beslemenin negatif ilişkili olduğunu göstermiştir. Sadece BSF ve TM ile beslenen tüm bireyler deneyin yaklaşık 30. gününde öldükleri gözlenmiştir. Kerevitlerin deneme sonu canlı ağırlığı, yemden yararlanma oranı, kabuk değiştirme ve yaşama oranı 30. günde gruplar arasında önemli farklılıklar göstermiştir (P < 0,05). CF+BSFL ve CF+TM ile beslenen kerevitlerin ağırlık, FCR, kabuk değiştirme oranı, tek çeliped yaralanması ve kontrol grubu ile 30. gün ve 60. günde benzer olduğu görülmüştür. Ancak en yüksek yaşama oranı CF ile beslenen kerevitlerde gözlenmiş, bu grubu 60. günde CF+BSFL ve CF+TM grupları takip etmiştir. Sonuç olarak, tek başına taze olarak verilen TM ve BSF besinleri kerevit larvalarında büyüme ve yaşama oranı üzerinde olumsuz sonuçlar göstermiştir. Ticari yemlerin %50 oranında azaltılması ve ek yem olarak kullanılmasıyla TM ve BSF larvaları kontrol grubu ile benzer büyüme gösterebilmektedir. Ancak yaşama oranlarının azalması nedeniyle ticari yemlerde %50 oranında TM eklenmesi önerilmemektedir. Taze BSF, P. astacus larvalarına, yaşama oranı ve büyümeyi etkilemeden ticari yemi %50 oranında azaltarak verilebilir.

Anahtar Kelimeler

Büyüme, Hermetia illucens, Pontastacus leptodactylus, Tenebrio molitor, Yaşama oranı

Growth Performance and Survival Rate of Pontastacus leptodactylus Juveniles Fed with Fresh Black Soldier Fly Hermetia illucens and Mealworm Tenebrio molitor Larvae

Öz

In this study, the survival rate and growth performance of crayfish Pontastacus leptodactylus juveniles were evaluated when fed with five different diets. These diets were CF: commercial feed, Hİ: Fresh Hermetia illucens, CF+Hİ; 50% commercial feed + 50% fresh Hermetia illucens, TM: Fresh Tenebrio molitor, CF+TM: 50% commercial feed + 50% fresh Tenebrio molitor. The experiments of the present study were carried out for 60 days. The results showed that the survival rate, final body weight (FBW), and weight gain (WG) were negatively correlated with fresh BSF and TM diets. All individuals fed only with BSF and TM diets died on approximately the 30th day of the experiment. The final weight, feed conversion ratio (FCR), molting rate and survival rate of crayfish fed with dietary treatment diets showed significant differences on the 30th day (P < 0.05). The crayfish fed with CF+BSFL and CF+TM diets had similar weight, FCR, molting rate, single cheliped injury with the control group on the 30th day and 60th day. However, the highest survival rate was observed in crayfish fed with CF diet followed by CF+BSFL and CF+TM groups on the 60th day. As a result, TM and BSF diets given fresh alone showed negative effects on growth and survival rates in crayfish larvae. Therefore using just fresh insects in the crayfish juvenile diets is not recommended. By decreasing 50% commercial feeds and using as supplementary feed of TM and BSF larvae can show similar growth as the control group. However, 50% TM supplemented with 50% commercial diets is not recommended due to reduced survival rates. On the other hand, fresh BSF can be given to P. astacus larvae after reducing the commercial feed by 50% without affecting the survival rate and growth.

Anahtar Kelimeler

Growth, Hermetia illucens, Pontastacus leptodactylus, Survival rate, Tenebrio molitor

Kaynakça

• AOAC. (2004). Official Methods of Analysis. 18th ed. Association of Official Analytical Chemists, Arlington, VA, USA.
• Cai, Y., Huang, H., Yao, W., Yang, H., Xue, M., Li, X., & Leng, X. (2022). Effects of fish meal replacement by three protein sources on physical pellet quality and growth performance of Pacific white shrimp (Litopenaeus vannamei). Aquaculture Reports, 25, (1-8), 101210. https://doi.org/10.1016/j.aqrep.2022.101210
• Celada, J. D., de Paz, P., Gaudioso, V. R., & Fernández, R. (1987). Embryonic development of the freshwater crayfish (Pacifastacus leniusculus Dana): a scanning electron microscopic study. The Anatomical Record, 219(3), 304-310. https://doi.org/10.1002/ar.1092190311
• Chen, Y., Chi, S., Zhang, S., Dong, X., Yang, Q., Liu, H., ... & Xie, S. (2022). Effect of black soldier fly (Hermetia illucens) larvae meal on lipid and glucose metabolism of Pacific white shrimp Litopenaeus vannamei. British Journal of Nutrition, 128(9), 1674-1688. https://doi.org/10.1017/S0007114521004670
• Chen, X., Feng, Y., & Chen, Z. (2009). Common edible insects and their utilization in China. Entomological Research, 39(5), 299-303. https://doi.org/10.1111/j.1748-5967.2009.00237.x
• Choi, I. H., Kim, J. M., Kim, N. J., Kim, J. D., Park, C., Park, J. H., & Chung, T. H. (2018). Replacing fish meal by mealworm (Tenebrio molitor) on the growth performance and immunologic responses of white shrimp (Litopenaeus vannamei). Acta Scientiarum. Animal Sciences, 40, e39077. https://doi.org/10.4025/actascianimsci.v40i1.39077
• Conti, F., Zarantoniello, M., Antonucci, M., Cattaneo, N., Rattin, M., De Russi, G., ... & Olivotto, I. (2023). The application of synthetic flavors in zebrafish (Danio rerio) rearing with emphasis on attractive ones: Effects on fish development, welfare, and appetite. Animals, 13(21), 3368. https://doi.org/10.3390/ani13213368
• Feng, P., He, J., Lv, M., Huang, G., Chen, X., Yang, Q., ... & Ma, H. (2019). Effect of dietary Tenebrio molitor protein on growth performance and immunological parameters in Macrobrachium rosenbergii. Aquaculture, 511, (1-9) 734247. https://doi.org/10.1016/j.aquaculture.2019.734247
• Foysal, M. J., Fotedar, R., Tay, C. Y., & Gupta, S. K. (2019). Dietary supplementation of black soldier fly (Hermetica illucens) meal modulates gut microbiota, innate immune response and health status of marron (Cherax cainii, Austin 2002) fed poultry-by-product and fishmeal based diets. PeerJ, 7, e6891. https://doi.org/10.7717/peerj.6891
• Foysal, M. J., Fotedar, R., Siddik, M. A., Chaklader, M. R., & Tay, A. (2021). Lactobacillus plantarum in black soldier fly (Hermetica illucens) meal modulates gut health and immunity of freshwater crayfish (Cherax cainii). Fish & Shellfish Immunology, 108, 42-52. https://doi.org/10.1016/j.fsi.2020.11.020
• He, Y., Liu, X., Zhang, N., Wang, S., Wang, A., Zuo, R., & Jiang, Y. (2022). Replacement of commercial feed with fresh black soldier fly (Hermetia illucens) larvae in Pacific white shrimp (Litopenaeus vannamei). Aquaculture Nutrition, 2022. https://doi.org/10.1155/2022/9130400
• Herawati, V. E., Susilo, A., Pinandoyo, P., Hutabarat, J., Wirasatriya, A., & Radjasa, O. K. (2019). Optimization of fish meal substitution with maggot meal (Hermetia illucens) for growth and feed utilization efficiency of juvenile Litopenaeus vannamei. Asian Journal of Microbiology, Biotechnology & Environmental Sciences, 21(2), 284-297.
• Herawati, V. E., Jayanti, H. T., Elfitasari, T., Putro, S. P., & Windarto, S. (2023). Black soldier fly (Hermetia illucens) oil inclusion and its effects on growth performances in common carp (Cyprinus carpi. Scientific Journal of Fisheries & Marine/Jurnal Ilmiah Perikanan dan Kelautan, 15(2), 361-373.
• Kroeckel, S., Harjes, A. G., Roth, I., Katz, H., Wuertz, S., Susenbeth, A., & Schulz, C. (2012). When a turbot catches a fly: Evaluation of a pre-pupae meal of the Black Soldier Fly (Hermetia illucens) as fish meal substitute growth performance and chitin degradation in juvenile turbot (Psetta maxima). Aquaculture, 364, 345-352. https://doi.org/10.1016/j.aquaculture.2012.08.041
• Lalander, C. H., Fidjeland, J., Diener, S., Eriksson, S., & Vinnerås, B. (2015). High waste-to-biomass conversion and efficient Salmonella spp. reduction using black soldier fly for waste recycling. Agronomy for Sustainable Development, 35, 261-271. https://doi.org/10.1007/s13593-014-0235-4
• Li, S., Ji, H., Zhang, B., Zhou, J., & Yu, H. (2017). Defatted black soldier fly (Hermetia illucens) larvae meal in diets for juvenile Jian carp (Cyprinus carpio var. Jian): Growth performance, antioxidant enzyme activities, digestive enzyme activities, intestine and hepatopancreas histological structure. Aquaculture, 477, 62-70. https://doi.org/10.1016/j.aquaculture.2017.04.015
• Mastoraki, M., Katsika, L., Enes, P., Guerreiro, I., Kotzamanis, Y. P., Gasco, L., ... & Antonopoulou, E. (2022). Insect meals in feeds for juvenile gilthead seabream (Sparus aurata): Effects on growth, blood chemistry, hepatic metabolic enzymes, body composition and nutrient utilization. Aquaculture, 561, 738674. https://doi.org/10.1016/j.aquaculture.2022.738674
• Mazlum, Y., Turan, F., & Bircan Yıldırım, Y. (2021). Evaluation of mealworms (Tenebrio molitor) meal as an alternative protein source for narrow‐clawed crayfish (Pontastacus leptodactylus) juveniles. Aquaculture Research, 52(9), 4145-4153. https://doi.org/10.1111/are.15253
• Mazlum, Y., & Uzun, C. (2022). Impact of stocking density on the survival, growth and injury of Narrow-Clawed Crayfish (Pontastacus leptodactylus) reared in a flowing brackish water system. Acta Natura & Scientia, 3(2) 163-183. https://doi.org/10.29329/actanatsci.2022.352.08
• Motte, C., Rios, A., Lefebvre, T., Do, H., Henry, M., & Jintasataporn, O. (2019). Replacing fish meal with defatted insect meal (Yellow Mealworm Tenebrio molitor) improves the growth and immunity of pacific white shrimp (Litopenaeus vannamei). Animals, 9(5), 258. https://doi.org/10.3390/ani9050258
• Mousavi, S., Zahedinezhad, S., & Loh, J. Y. (2020). A review on insect meals in aquaculture: The immunomodulatory and physiological effects. International Aquatic Research, 12(2), 100-115. https://doi.org/10.22034/IAR(20).2020.1897402.1033
• Muslimin, B., Yonarta, D., Heriyati, E., Helmizuryani, H., Sari, M. P., Apriyanti, D., & Kurniawan, R. (2023). Impact of black soldier fly (Hermetia illucens) fresh meal on the growth performance, digestive enzymes, hematology, and intestinal histology of cork fish (Channa striata). Turkish Journal of Veterinary & Animal Sciences, 47(4), 324-333. https://doi.org/10.55730/1300-0128.4301
• Sánchez‐Muros, M. J., Renteria, P., Vizcaino, A., & Barroso, F. G. (2020). Innovative protein sources in shrimp (Litopenaeus vannamei) feeding. Reviews in Aquaculture, 12(1), 186-203. https://doi.org/10.1111/raq.12312
• Ordoñez, B. M., Santana, T. M., Carneiro, D. P., dos Santos, D. K., Parra, G. A., Moreno, L. C., ... & Gonçalves, L. U. (2022). Whole black soldier fly larvae (Hermetia illucens) as dietary replacement of extruded feed for tambaqui (Colossoma macropomum) juveniles. Aquaculture Journal, 2(4), 246-256.
• Richardson, A., Dantas-Lima, J., Lefranc, M., & Walraven, M. (2021). Effect of a black soldier fly ingredient on the growth performance and disease resistance of juvenile pacific white shrimp (Litopenaeus vannamei). Animals, 11(5), 1450. https://doi.org/10.3390/ani11051450
• Wang, T., Wang, X., Shehata, A. I., Wang, R., Yang, H., Wang, Y., ... & Zhang, Z. (2022). Growth performance, physiological and antioxidant capacity responses to dietary fish meal replacement with insect meals for aquaculture: A case study in red claw crayfish (Cherax quadricarinatus). Aquaculture Research, 53(10), 3853-3864. https://doi.org/10.1111/are.15892
• Yu, Z., Li, L., Zhu, R., Li, M., Duan, J., Wang, J. Y., ... & Wu, L. F. (2020). Monitoring of growth, digestive enzyme activity, immune response and water quality parameters of Golden crucian carp (Carassius auratus) in zero-water exchange tanks of biofloc systems. Aquaculture Reports, 16, 100283. https://doi.org/10.1016/j.aqrep.2020.100283
• Zarantoniello, M., Chemello, G., Ratti, S., Pulido-Rodríguez, L. F., Daniso, E., Freddi, L., ... & Olivotto, I. (2023). Growth and welfare status of giant freshwater prawn (Macrobrachium rosenbergii) post-larvae reared in aquaponic systems and fed diets including enriched black soldier fly (Hermetia illucens) prepupae meal. Animals, 13(4), 715.