Determination of Oxidative Stress Responses in Dreissena polymorpha Exposed to Rare Earth Elements (Terbium, Lanthanum, Gadolinium and Praseodymium) with Temperature

Yıl 2025, Cilt: 21 Sayı: 2, 145 - 157

Ayşe Nur Aydın , Osman Serdar

https://doi.org/10.22392/actaquatr.1585378

Öz

The continuous development of industry pushes people to search for new resources, and for this reason, the usage areas of Rare Earth Elements (REEs) are increasing day by day. Increasing concentrations of REEs, as a result of increased use, create pollution in the environment and harm living organisms. This pollution interacts with increasing temperature and causes more negative synergistic effects of the pollutant in the environment and in the living body. In this study, sublethal concentration values were determined by literature review and the concentration value was determined as 125 mg/L. In the present study tt was aimed to investigate some oxidative stress and antioxidant responses of Terbium, Lanthanum, Gadolinium and Praseodymium REEs in Dreissena polymorpha at 125 mg/L concentration at 3 different temperatures (16, 18, 20 0C) with biomarkers. For this purpose, 24 and 96 hour experimental trial design was created and 7 D. polymorpha were used in each trial group, and the application experiments were carried out with 3 replications. The samples at the end of the experimental phase were stored at -80 degrees Celsius until they were analyzed. In this study, Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzyme activities and glutathione (GSH) and Thiobarbituric acid (TBARS) level biomarker responses were determined by ELISA test microplate reader. CAYMAN brand SOD (Catalog No 706002), CAT Catalog No 707002) and GPx (Catalog No 703102), GSH (Catalog No 703002) and TBARS (Catalog No 10009055) were used in the study. SPSS 24.0 package program one-way ANOVA (Duncan 0.05) was used for the evaluation of biochemical analyzes. According to the study data, statistically significant decreases were observed in SOD and CAT activities in the oxidative stress responses of REEs on D. Polymorpha with increasing temperature, while there was no significant change in GPx activities. It was determined that there were increases in TBARS levels and decreases in GSH levels. It is thought that the temperature factor, application concentration and application time are effective in the formation of these changes. It can be said that temperature change and pollutants cause oxidative stress in organisms and cause cell damage.

Anahtar Kelimeler

Dressena polymorpha , Tempareture , Oksidative stress , Rare Earth Elements

Sıcaklıkla Birlikte Nadir Toprak Elementlerine (Terbium, Lantan, Gadolinyum ve Praseodimyum) Maruz Bırakılan Dreissena polymorpha'da Oksidatif Stres Tepkilerinin Belirlenmesi

Öz

Sanayi ve endüstrinin sürekli gelişmesi insanları yeni kaynak arayışlarına itmekte ve bu amaçla kullanım alanları her geçen gün artan Nadir Toprak Elementlerinin (NTE) kullanım alanları hızla artmaktadır. Artan kullanım sonucu NTE'lerin artan konsantrasyonları çevrede kirlilik yaratmakta ve canlı organizmalara zarar vermektedir. Bu kirlilik artan sıcaklıkla etkileşime girerek kirleticinin çevrede ve canlı vücudunda daha olumsuz sinerjik etkilere neden olmaktadır. Bu çalışmada, subletal konsantrasyon değerleri literatür taraması ile belirlenmiş ve konsantrasyon değeri 125 mg/L olarak tespit edilmiştir. Dreissena polymorpha'da Terbium, Lanthanum, Gadolinium ve Praseodymium NTE'lerin 125 mg/L konsantrasyonda 3 farklı sıcaklıkta (16, 18, 20 0C) bazı oksidatif stres ve antioksidan tepkilerinin biyobelirteçler ile araştırılması amaçlanmıştır. Bu amaçla 24 ve 96 saatlik deneme deseni oluşturularak her deneme grubunda 7 adet D. polymorpha kullanılmış ve uygulama deneyleri 3 tekrarlı olarak gerçekleştirilmiştir. Deneme aşaması biten örnekler analiz edilene kadar -80 derecede muhafaza edilmiştir. Bu çalışmada Süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GPx) enzim aktiviteleri ile glutatyon (GSH) ve Tiyobarbitürik asit (TBARS) düzeyi biyobelirteç yanıtları ELISA testi mikroplaka okuyucu ile belirlenmiştir. Çalışmada CAYMAN marka SOD (Katalog No 706002), CAT Katalog No 707002) ve GPx (Katalog No 703102), GSH (Katalog No 703002) ve TBARS (Katalog No 10009055) kullanılmıştır. Biyokimyasal analizlerin değerlendirilmesi için SPSS 24.0 paket programı tek yönlü ANOVA (Duncan 0.05) kullanılmıştır. Çalışma verilerine göre, D. Polymorpha üzerinde NTE'lerin oksidatif stres tepkilerinde artan sıcaklıkla birlikte SOD ve CAT aktivitelerinde istatistiksel olarak anlamlı düşüşler gözlenirken, GPx aktivitelerinde anlamlı bir değişiklik olmamıştır. TBARS seviyelerinde artışlar, GSH seviyelerinde ise düşüşler olduğu tespit edilmiştir. Bu değişikliklerin oluşumunda sıcaklık faktörü, uygulama konsantrasyonu ve uygulama süresinin etkili olduğu düşünülmektedir. Sıcaklık değişiminin ve kirleticinin organizmalarda oksidatif strese neden olduğu ve hücre hasarına yol açtığı söylenebilir.

Anahtar Kelimeler

Dreissena polymorpha , Sıcaklık , Oksidatif stres , Nadir Toprak Elementleri

Etik Beyan

All authors declare that there is no ethical violation in this manuscript. Also, this manuscript does not contain data belonging to others. The authors declare that they have no conflict of interest. The authors alone are responsible for the content and authoring of the present paper.

Destekleyen Kurum

No support was received from any institution or organization.

Kaynakça

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