Immune Precipitation Technique to Detect ABO Antigens from Dry Bloodstain

Öz

A new precipitation technique has been developed for detecting immune complexes using a synthetic polymer, namely polyethylene glycol (PEG) and applied for the detection of ABO antigens from dry bloodstains. Fresh Iysates and extracts from 16 days old stains were tested and the ABO antigens could be detected at PEG concentrations of 10 % to 4, %. The anti-A and anti-B antibodies were soluble in 10 % PEG whereas the immune complexes formed by these antibodies were precipitated at that concentration. The immnne precipitate caused by anti·A and anti-B antibodies were detectable up to a PEG concentration of 7 %. The Ulex leetin against H antigen was also soluble at 10 % PEG. The complex formed by this le etin could be precipitated by PEG upto a concentration of 5 %. When compared to the existing methods this immune precipitation techniqu e using PEG appears simple and reliable. With certain modification this tecnique can be used for the detection of ABD antigens from other body fluids and their stains. There exists the possibility that the HLA antigens can also be detected by this technique.

Anahtar Kelimeler

• Immune precipitation
• ABO antigens
• Bloodstain

Kaynakça

1. Farr, R.S. (1958) J. Infect. Dis., 103, 239 (loc. cit. Creighton, W.D. et aL., J. ImmunoI., lll, 1219, 1973).
2. Hell.ing, K. (1966) Acta Chem. Scand., 20, 1251 (loc. cit. diııo).
3. Stahman, M.A., Mathews, R.E.F. (1954) J. ImmunoL., 72, 435 (loc. cit. diııo).
4. Harrington, J.C., Fenton, J.W., Pert, J.H. (1971) Immunochemistry, 8, 413 (loc. cit. diııo ).
5. Creighton, W.D., Lambert, P.H., Miescher, P.A. (1973) J. ImmunoL., lll, 1219.
6. Lee, H.C. (1982) in Forensic Science Handbook, (Saferstein, H., cd) pp. 297 - 337, Prentice Hall International Ine., New Jer5ey.