DETERMINATION AND METHOD VALIDATION OF HEROIN, MORPHINE, CODEINE AND 6-MONOACETYLMORPHINE BY GC-FID

Yıl 2011, Cilt: 25 Sayı: 2, 50 - 53, 31.08.2011

Gürol Berber Mesut Şahin Eyüp Kandemir Muhammet Nabi Kantarcı Neval Berber

https://izlik.org/JA28RZ25KZ

Öz

Objective:
This study describes the
development and validation of
a gas chromatography–flame
ionization detection (GC–FID)
method for the quantitative heroin
(diacetylmorphine), morphine,
codeine (3-methylmorphine) and
6-monoacetylmorphine (6 MAM) in
narcotics.
Methods:
In our study, GC/FID Agilent 6890N
(DB-5: 10,0m x 100 µm x 0,17
µm) with Chemstation software
and 5% phenylmethylpolysiloxane
non polar analytic colon
(max 325 °C) was used for
the analyses. Standards and
reagents were Dichloromethaneisopropylalcohol mixture (1:
1), Methanol, HeroinHCl.H2O,
CodeineHCI, MorphineHCI,
6-MonoacetylmorphineHCI,
Paracetamol, caffeine and
n-docosane which were used as
reference standard and internal
standard. Carrier gas was Helium.
Injection temperature (T) was
280 ˚C, injection volume was 1 µl.
Repeatability study was evaluated
using 4 operators. Reproducibility
study was evaluated using 3
operators during 29 days.
Results:
The method is linear over the
range (0.01-2.00mg mL-1) for
heroin, (0,008-2 mg L-1) for
codeine, (0,005-2 mg mL-1) for
6-monoacetylmorphine and (0.015-
0,75mg mL-1) for morphine in the
study. Correlation coefficients
vary between 0.997 and 0.999 are
detected.
Conclusion:
The increases in demanding of
human rights, in getting prevalent
of special insurances make
it obligatory to perform total
quality principles particularly for
forensic disciplines. Reliability
and credibility are also found very
important by the courts. For this
reason validation and continual
reclamation of methods that have
been used in forensic laboratories
is getting very important.

Anahtar Kelimeler

Laboratory , Quality , Validation , Forensic

Kaynakça

• Small LF, Lutz RE, editors. Chemistry of the Opium Alkaloids. U. S. Government Printing Office: Washington: DC. 1932: 153–154.
• Rapoport H. The Preparation of Morphine-N-Methyl-C14. J. Am. Chem. Soc. 1951;73:5900.
• Crews JC, Denson DD, editors. Recovery of morphine from a controlled-release preparation. A source of opioid abuse. Cancer, 1990; 66:2642–4.
• Trescot AM, Datta S, Lee M, et all. Opioid pharmacology. Pain Physician, 2008; 11:133–53.
• Baselt R, editor. Disposition of Toxic Drugs and Chemicals in Man, CA: Biomedical Publications, 8th ed. Foster City, 2008: 1057-1062.
• Shewan D, Dalgarno P. Evidence for contolled heroin use? High levels of negative health and social outcomes among non-treatment heroin users in glasgow. British Journal of Health Psychology, 2005; 10:33–48.
• Hook S, editor. The Hero in History. A Study in Limitation and Possibility. MA: Beacon Press, Boston, 1955:92-21.
• Sawynok J. The therapeutic use of heroin: a review of the pharmacological literature. Canadian Journal of Physiology and Pharmacology, 1986; 64:1- 6.
• Klous MG, Van den Brink W, Van Ree JM et all. Development of pharmaceutical heroin preparations for medical coprescription to opioid-dependent patients. Drug and Alcohol Dependence. 2005; 12;283-95.
• Srinivasan V, Wielbo D, Tebbett IR. Analgesic effects of codeine-6-glucuronide after intravenous administration. European journal of pain, 1997; 1:185–90.
• Tasker RA, Vander Velden PL, Nakatsu K. Relative cataleptic potency of narcotic analgesics, including 3,6-dibutanoylmorphine and 6-monoacetylmorphine. Prog Neuropsychopharmacol Biol Psychiatry, 1984; 8:747-50.
• CITAC/EURACHEM, 2002. Guide to quality in analytical chemistry: an aid to accreditation. CITAC/EURACHEM Guide, Edition: 2002.
• ISO/IEC 17025, General Requirements for the Competence of Testing and Calibration Laboratories. ISO, Geneva, Switzerland: 1999.
• Manual for use by national drug testing laboratories. Methods for impurity profiling heroin and cocaine, New York: United Nations, 2005: 13-45.