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ADLİ TIP UYGULAMALARINDA YARA YAŞI VE CANLILIK BULGULARININ DEĞERLENDİRİLMESİ

Yıl 2011, Cilt: 25 Sayı: 2, 69 - 76, 31.08.2011
https://izlik.org/JA88SA37SP

Öz

Yaraların değerlendirilmesi Adli
tıp pratiğinin en önemli uygulama alanlarındandır. Adli tıp uygulamalarında otopsi sırasında
saptanan bir yaranın ölümden
önce mi, yoksa ölümden sonra
mı meydana geldiğini (canlılık)
ve ölümden önce ise, ölümden
ne kadar önce meydana gelmiş olduğunu (yara yaşı) tespit
etmek son derece önemlidir.
Yaranın özelliklerini doğru ve
objektif bir şekilde tanımlamak,
yara canlılığını ve yaşını saptayabilmek adli sürecin doğru işleyebilmesi için gereklidir. Bu
nedenlerle canlılık ve yara yaşı
değerlendirmeleri adli tıp alanındaki araştırmaların en ilgi
çeken konularındandır. Yara iyileşmesi basit ancak iyi organize
olmuş biolojik bir süreçtir. İnflamasyon, proliferasyon ve maturasyon olmak üzere birbirini
takip eden üç süreçten oluşur.
Çok sayıda biolojik madde yara
iyileşme sürecinde rol almaktadır; bu kısa ve basit yara iyileşme süreci adli tıpta yara yaşı ve
yara canlılığının saptanmasında
kullanılabilir.
Son yıllarda immünhistokimyanın ve kimyasal tekniklerin
gelişmesiyle bu alan hızla ilerlemiştir. Günümüzde yara yaşı
ve canlılık soruları üzerinde
çalışmak için temel olarak immünohistokimyasal yöntemler,
biyokimyasal yöntemler ve moleküler biyolojik teknikler kullanılmaktadır.
Çalışmada yara yaşı ve canlılık
değerlendirmesinin adli tıp uygulamalarındaki yeri, yara yaşı
ve canlılık belirlenmesinde kullanılan yöntemler, bu alandaki
gelişmeler ve uygulamadaki sorunlar tartışılmıştır.

Kaynakça

  • Wolfgang G, Burkhard M. Demands on scientific studies: Vitality of wounds and wound age estimation. Forensic Sci Int 2007 17;165(2-3): 150-154.
  • Oehmichen M. Vitality and time course of wounds. Forensic Sci Int 2004;10;144(2-3): 221- 231.
  • Çetin G. Yaralar Adli Tıp Kitabı Cilt 1. 1. Baskı. İ Ü Cerrahpaşa Tıp Fakültesi Yayınevi İstanbul 1999;475-523.
  • Knight B. The pathology of wounds. Chapter 4. In: Saukko P, Knight B, eds. Forensic Pathology. third ed. Oxford University Press Inc, 2004;163-69.
  • Ohshima T. Forensic Wound Examination. Forensic Sci Int 2000;113: 153 -164.
  • Ekizoğlu O. Arıcan N. Yaralar. Klinik Gelişim 2009; 22: 33-43.
  • Kondo T. Timing of skin wounds. Legal Medicine 2007;9(2): 109-14.
  • Betz P. Pathophysiology of wound healing. Chapter 17. In: James JP, Busutttil A, Smock W,eds. Forensic Medicine Clinical and Pathological Aspects. London 2003; 83-89.
  • Polat O, İnanıcı MA, Aksoy ME. Yaralar, Adli Tıp Ders Kitabı. Nobel Tıp Kitabevleri, 1997;101- 250.
  • Dimaio VJ, Dimaio D. Blunt trauma wounds. In: Dimaio VJ, Dimaio D, eds. Forensic Pathology. 2nd ed. Florida, USA: CRC Press LLC, 2001: 92-116.
  • Raekallio J. Histological estimation of the age of injuries. In: Perper JA, Wechts CH, eds. Microscopic Diagnosis in Forensic Pathology, Thomas, Springfield/IL, 1975; 3–16.
  • Berg S, Ditt J, Friedrich D, et al. Möglichkeiten der biochemischen Wundaltersbestimmung. Dtsch Z Gerichtl Med 1968; 63: 183–198.
  • Berg S, Bonte W. Praktische Erfahrungen mit der biochemischen Wundaltersbestimmung. Beitr Gerichtl Med 1971;28: 108–114.
  • Fieguth A, Feldbrugge H, Gerich T, et al. The time-dependent expression of fibronectin, MRP8, MRP14 and defensin in surgically treated human skin wounds. Forensic Sci Int 2003;131: 156–161.
  • Fieguth A, Kleemann WJ, Troger HD. Immunohistochemical examination of skin wounds with antibodies against alpha1-antichymotrypsin, alpha-2- macroglobulin and lysozymem. Int J Legal Med 1994;107: 29–33.
  • Dressler J, Bachmann L, Kasper M, et al. Time dependence of the expression of ICAM-1 (CD 54) in human skin wounds. Int J Legal Med 1997; 110: 299–304.
  • Dressler J, Bachmann L, Koch R, et al. Enhanced expression of selectins in human skin wounds. Int J Legal Med 1999;112: 39–44.
  • Dressler J, Bachmann L, Strejc P, et al. Expression of adhesion molecules in skin wounds: diagnostic value in legal medicine. Forensic Sci Int 2000; 113: 173–176.
  • Grellner W, Dimmeler S, Madea B. Immunohistochemical detection of fibronectin in postmortem incised wounds of porcine skin. Forensic Sci Int 1998; 97: 109–116.
  • Hernández-Cueto C, Lorente JA, Pedal I, et al. Cathepsin D as a vitality marker in human skin wounds. Int J Legal Med 1993; 106: 145–147.
  • Hernández-Cueto C, Girela E, Sweet DJ. Advances in the diagnosis of wound vitality: a review. Am J Forensic Med Pathol 2000; 21: 21–31.
  • Hernández-Cueto C, Vieira DN, Girela E, et al. Diagnostic ability of D-dimer in the establishment of the vitality of wounds. Forensic Sci Int 1995; 76: 141–149.
  • Ortiz-Rey JA, SuárezKAYNAKLAR Peñaranda JM, Muñoz-Barús HI, et al. Expression of fibronectin and tenascin as a demonstration of vital reaction in rat skin and muscle. Int J Legal Med 2003; 117: 356–360.
  • Kondo T, Ohshima T, Eisenmenger W. Immunohistochemical and morphometrical study on the temporal expression of interleukin-1 (IL-1 ) in human skin wounds for forensic wound age determination. Int J Legal Med 1990; 112: 249–252.
  • Janssen W. Wound healing in Janssen W. ed. Forensic histopathology. Springer-Verlag Berlin Heidelberg New York Tokyo;1984: 81-97.
  • Mitchell RN, Cotran RS. Acute and Chronic İnflammation. Chapter 2. In: Kumar V, Cotran RS, Robbins SL, eds. Basic Pathology. 7th ed. USA 2005, 47-86.
  • Pakiş I, Akyıldız EÜ, Turan AA, Karayel F, Ersoy G, Kır Z. Miyokard İnfarktüsünde Mast Hücrelerinin Rolü; Otopsi Çalışması. Adli Tıp Bülteni 2008:13(1):9-12.
  • Akyıldız EÜ, Pakiş I, Ersoy G, Karayel F, Turan AA. Komplike atheromatöz plak gelişiminde mast hücrelerinin rolü. Sayfa:125. VII.Adli Bilimler Kongresi 11-14 Mayıs 2006, Konya.
  • Hughes VK, Ellis PS, Langlois NE. Alternative light source (polilight) illumination with digital image analysis does not assist in determining the age of bruises. Forensic Sci Int 2006 10;158(2-3): 104-7.
  • Hughes VK, Ellis PS, Burt T, et al. The practical application of reflectance spectrophotometry for the demonstration of haemoglobin and its degradation in bruises. J Clin Pathol 2004;57(4): 355-9.
  • Yajima Y, Nata M, Funayama M. Spectrophotometric and tristimulus analysis of the colors of subcutaneous bleeding in living persons. Leg Med (Tokyo) 2003;5(1): 342-3.
  • Stephenson T, Bialas Y. Estimation of the age of bruising. Arch Dis Child 1996;74(1): 53-5.
  • Psaroudakis K, Tzatzarakis MN, Tsatsakis AM, et al. The application of histochemical methods to the age evaluation of skin wounds: experimental study in rabbits. Am J Forensic Med Pathol 2001;22(4): 341-5.
  • Cordell JL, Falini B, Erber WN, et al. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984; 32: 219–229.
  • Haussman R. Betz P. The time coursa of the vaskular response to human brain injury-and immunohistochemical study. Int J Legal Med 2000;113: 288-292.
  • Balazic J, Grajn A, Kralj E, et al. Expression of fibronectin suicidal in gun shot wounds. Forensic Sci Int 2005; 17(147): 5-7.
  • Çelik S, Pakiş I, Yorulmaz Ç, Koç S. Immunhistochemical Study for Postmortem Diagnosis of Early Myocardial Infarction. p:28. 18th Triennial Meeting, New Orleans, Lousiana, USA July 21-25, 2008.
  • Dreßler J, Bachmann L, Strejc P, et al. Expression of adhesion molekules in skin wounds: diagnostic value in legal medicine. Forensic Sci Int 2000;113: 173-176.
  • Dreßler J, Bachmann L, Kasper M, et al. Enhanced expression of ICAM-1 (CD 54) in human skin wounds: diagnostic value in legal medicine. Inflamm Res 1997;46: 434-435.
  • Dreßler J, Bachmann L, Koch R, et al. Estimation of wound age and VCAM-1 in human skin, Int J Legal Med 1999;112: 159-162.
  • Dreßler J, Bachmann L, Koch R, et al. Enhanced expression of selectins in human skin wounds. Int J Legal Med 1998;112: 39–44.
  • Dreßler J, Bachmann L, Kasper M, et al. Time dependence of the expression of ICAM-1 (CD 54) in human skin wounds. Int J Legal Med 1997;110: 299–304.
  • Takamiya M, Fujita S, Saigusa K, et al. Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation. Int J Legal Med 2008;122(2): 143-8.
  • Takamiya M, Fujita S, Saigusa K, et al. Simultaneous detections of 27 cytokines during cerebral wound healing by multiplexed bead-based immunoassay for wound age estimation. J Neurotrauma 2007;24(12): 1833-44.
  • Grellner W. Time-dependent immunohistochemical detection of proinflammatory cytokines (IL-1beta, IL-6, TNF-alpha) in human skin wounds. Forensic Sci Int 2002: 4;130(2-3): 90-6.
  • Grellner W, Georg T, Wilske J. Quantitative analysis of proinflammatory cytokines (IL1beta, IL-6, TNF-alpha) in human skin wounds. Forensic Sci Int 2000: 11;113(1-3): 251-64.
  • Kondo T, Ohshima T, Eisenmenger W. Immunohistochemical and morphometrical study on the temporal expression of interleukin-1alpha (IL-1alpha) in human skin wounds for forensic wound age determination. Int J Legal Med 1999;112: 249–52.
  • Lin ZQ, Kondo T, Ishida Y, et al. Essential involvement of IL-6 in the skin wound-healing process as evidenced by delayed wound healing in IL-6-deficient mice. J Leukoc Biol 2003;73: 713–21.
  • Mori R, Kondo T, Ohshima T, et al. Accelerated wound healing in tumor necrosis factor receptor p55-deficient mice with reduced leukocyte infiltration. FASEB J 2002;16: 963–74.
  • Michel G, Kemeny L, Peter RU, et al. Interleukin-8 receptor-mediated chemotaxis of normal human epidermal cells. FEBS Lett 1992;305: 241–3.
  • Takamiya M, Saigusa K, Aoki Y. Immunohistochemical study of basic fibroblast growth factor and vascular endothelial growth factor expression for age determination of cutaneous wounds. Am J Forensic Med Pathol 2002;23(3): 264-7.
  • Hayashi T, Ishida Y, Kimura A, et al. Forensic application of VEGF expression to skin wound age determination. Int J Legal Med 2004;118: 320–5.
  • Gilbert L,Siang HL. Forensic histopathology chapter 13 part 8. In: Michael Tsokos, ed. Forensic pathology reviews, vol.5, 2008;240-64.
  • Grellner W, Vieler S, Madea B. Transforming growth factors (TGFalpha and TGF-beta1) in the determination of vitality and wound age: immunohistochemical study on human skin wounds. Forensic Sci Int 2005;153: 174– 80.
  • Ishida Y, Kondo T, Takayasu T, et al. The essential involvement of cross-talk between IFN-gamma and TGF-beta in the skin wound-healing process. J Immunol 2004: 1;172(3): 1848- 55.
  • Kondo T, Tanaka J, Ishida Y, et al. Ubiquitin expression in skin wounds and its application to forensic wound age determination. Int J Legal Med. 2002: 116(5): 267-72.
  • Quan L, Zhu BL, Oritani S, et al. Intranuclear ubiquitin immunoreactivity in the pigmented neurons of the substantia nigra in fire fatalities. Int J Legal Med 2001;114(6): 310-5.
  • Bond U, Schlesinger MJ. The chicken ubiquitin gene contains a heat shock promoter and expresses an unstable mRNA in heat-shocked cells. Mol Cell Biol 1986;6(12): 4602-10.
  • Quan L, Zhu BL, Ishida K, et al. Intranuclear ubiquitin immunoreactivity of the pigmented neurons of the substantia nigra in fatal acute mechanical asphyxiation and drowning. Int J Legal Med 2001;115(1): 6-11.
  • Ishida Y, Kimura A, Takayasu T, et al. Expression of oxygenregulated protein 150 (ORP150) in skin wound healing and its application for wound age determination. Int J Legal Med 2008;122(5): 409-14.
  • Ozawa K, Kondo T, Hori O, et al. Expression of the oxygen-regulated protein ORP150 accelerates wound healing by modulating intracellular VEGF transport. J Clin Invest 2001;108(1): 41-50.
  • Kuwabara K, Matsumoto M, Ikeda J, et al. Purification and characterization of a novel stress protein, the 150-kDa oxygen-regulated protein (ORP150), from cultured rat astrocytes and its expression in ischemic mouse brain. J Biol Chem 1996;271(9): 5025-32.
  • Hausmann R, Nerlich A, Betz P.The time-related expression of p53 protein in human skin wounds-a quantitative immunohistochemical analysis. Int J Legal Med 1998;111(4): 169-72
  • Sato Y, Ohshima T. The expression of mRNA of proinflammatory cytokines during skin wound healing in mice: a preliminary study for forensic wound age estimation (II). Int J Legal Med 2000;113(3): 140-5.
  • Takamiya M, Saigusa K, Nakayashiki N, et al. Studies on mRNA expression of basic fibroblast growth factor in wound healing for wound age determination. Int J Legal Med 2003;117(1): 46-50.
  • Takamiya M, Saigusa K, Kumagai R, et al. Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation. Int J Legal Med 2005;119(1): 16-21.
  • Kagawa S, Matsuo A, Yagi Y, et al. The time-course analysis of gene expression during wound healing in mouse skin. Leg Med (Tokyo) 2009;11(2): 70-75.
  • Sun JH, Wang YY, Zhang L, et al. Time-dependent expression of skeletal muscle troponin I mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation. Int J Legal Med 2010;124(1): 27- 33.
  • Takamiya M, Fujita S, Saigusa K, et al. A study on mRNA expressions of interleukin 10 during fracture healing for wound age determination. Leg Med (Tokyo) 2008;10(3): 131-7.
  • Betz P, Nerlich A, Tübel J, et al. Detection of cell death in human skin wounds of various ages by an in situ end labeling of nuclear DNA fragments. Int J Legal Med1997;110(5): 240-3.

Yıl 2011, Cilt: 25 Sayı: 2, 69 - 76, 31.08.2011
https://izlik.org/JA88SA37SP

Öz

Kaynakça

  • Wolfgang G, Burkhard M. Demands on scientific studies: Vitality of wounds and wound age estimation. Forensic Sci Int 2007 17;165(2-3): 150-154.
  • Oehmichen M. Vitality and time course of wounds. Forensic Sci Int 2004;10;144(2-3): 221- 231.
  • Çetin G. Yaralar Adli Tıp Kitabı Cilt 1. 1. Baskı. İ Ü Cerrahpaşa Tıp Fakültesi Yayınevi İstanbul 1999;475-523.
  • Knight B. The pathology of wounds. Chapter 4. In: Saukko P, Knight B, eds. Forensic Pathology. third ed. Oxford University Press Inc, 2004;163-69.
  • Ohshima T. Forensic Wound Examination. Forensic Sci Int 2000;113: 153 -164.
  • Ekizoğlu O. Arıcan N. Yaralar. Klinik Gelişim 2009; 22: 33-43.
  • Kondo T. Timing of skin wounds. Legal Medicine 2007;9(2): 109-14.
  • Betz P. Pathophysiology of wound healing. Chapter 17. In: James JP, Busutttil A, Smock W,eds. Forensic Medicine Clinical and Pathological Aspects. London 2003; 83-89.
  • Polat O, İnanıcı MA, Aksoy ME. Yaralar, Adli Tıp Ders Kitabı. Nobel Tıp Kitabevleri, 1997;101- 250.
  • Dimaio VJ, Dimaio D. Blunt trauma wounds. In: Dimaio VJ, Dimaio D, eds. Forensic Pathology. 2nd ed. Florida, USA: CRC Press LLC, 2001: 92-116.
  • Raekallio J. Histological estimation of the age of injuries. In: Perper JA, Wechts CH, eds. Microscopic Diagnosis in Forensic Pathology, Thomas, Springfield/IL, 1975; 3–16.
  • Berg S, Ditt J, Friedrich D, et al. Möglichkeiten der biochemischen Wundaltersbestimmung. Dtsch Z Gerichtl Med 1968; 63: 183–198.
  • Berg S, Bonte W. Praktische Erfahrungen mit der biochemischen Wundaltersbestimmung. Beitr Gerichtl Med 1971;28: 108–114.
  • Fieguth A, Feldbrugge H, Gerich T, et al. The time-dependent expression of fibronectin, MRP8, MRP14 and defensin in surgically treated human skin wounds. Forensic Sci Int 2003;131: 156–161.
  • Fieguth A, Kleemann WJ, Troger HD. Immunohistochemical examination of skin wounds with antibodies against alpha1-antichymotrypsin, alpha-2- macroglobulin and lysozymem. Int J Legal Med 1994;107: 29–33.
  • Dressler J, Bachmann L, Kasper M, et al. Time dependence of the expression of ICAM-1 (CD 54) in human skin wounds. Int J Legal Med 1997; 110: 299–304.
  • Dressler J, Bachmann L, Koch R, et al. Enhanced expression of selectins in human skin wounds. Int J Legal Med 1999;112: 39–44.
  • Dressler J, Bachmann L, Strejc P, et al. Expression of adhesion molecules in skin wounds: diagnostic value in legal medicine. Forensic Sci Int 2000; 113: 173–176.
  • Grellner W, Dimmeler S, Madea B. Immunohistochemical detection of fibronectin in postmortem incised wounds of porcine skin. Forensic Sci Int 1998; 97: 109–116.
  • Hernández-Cueto C, Lorente JA, Pedal I, et al. Cathepsin D as a vitality marker in human skin wounds. Int J Legal Med 1993; 106: 145–147.
  • Hernández-Cueto C, Girela E, Sweet DJ. Advances in the diagnosis of wound vitality: a review. Am J Forensic Med Pathol 2000; 21: 21–31.
  • Hernández-Cueto C, Vieira DN, Girela E, et al. Diagnostic ability of D-dimer in the establishment of the vitality of wounds. Forensic Sci Int 1995; 76: 141–149.
  • Ortiz-Rey JA, SuárezKAYNAKLAR Peñaranda JM, Muñoz-Barús HI, et al. Expression of fibronectin and tenascin as a demonstration of vital reaction in rat skin and muscle. Int J Legal Med 2003; 117: 356–360.
  • Kondo T, Ohshima T, Eisenmenger W. Immunohistochemical and morphometrical study on the temporal expression of interleukin-1 (IL-1 ) in human skin wounds for forensic wound age determination. Int J Legal Med 1990; 112: 249–252.
  • Janssen W. Wound healing in Janssen W. ed. Forensic histopathology. Springer-Verlag Berlin Heidelberg New York Tokyo;1984: 81-97.
  • Mitchell RN, Cotran RS. Acute and Chronic İnflammation. Chapter 2. In: Kumar V, Cotran RS, Robbins SL, eds. Basic Pathology. 7th ed. USA 2005, 47-86.
  • Pakiş I, Akyıldız EÜ, Turan AA, Karayel F, Ersoy G, Kır Z. Miyokard İnfarktüsünde Mast Hücrelerinin Rolü; Otopsi Çalışması. Adli Tıp Bülteni 2008:13(1):9-12.
  • Akyıldız EÜ, Pakiş I, Ersoy G, Karayel F, Turan AA. Komplike atheromatöz plak gelişiminde mast hücrelerinin rolü. Sayfa:125. VII.Adli Bilimler Kongresi 11-14 Mayıs 2006, Konya.
  • Hughes VK, Ellis PS, Langlois NE. Alternative light source (polilight) illumination with digital image analysis does not assist in determining the age of bruises. Forensic Sci Int 2006 10;158(2-3): 104-7.
  • Hughes VK, Ellis PS, Burt T, et al. The practical application of reflectance spectrophotometry for the demonstration of haemoglobin and its degradation in bruises. J Clin Pathol 2004;57(4): 355-9.
  • Yajima Y, Nata M, Funayama M. Spectrophotometric and tristimulus analysis of the colors of subcutaneous bleeding in living persons. Leg Med (Tokyo) 2003;5(1): 342-3.
  • Stephenson T, Bialas Y. Estimation of the age of bruising. Arch Dis Child 1996;74(1): 53-5.
  • Psaroudakis K, Tzatzarakis MN, Tsatsakis AM, et al. The application of histochemical methods to the age evaluation of skin wounds: experimental study in rabbits. Am J Forensic Med Pathol 2001;22(4): 341-5.
  • Cordell JL, Falini B, Erber WN, et al. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984; 32: 219–229.
  • Haussman R. Betz P. The time coursa of the vaskular response to human brain injury-and immunohistochemical study. Int J Legal Med 2000;113: 288-292.
  • Balazic J, Grajn A, Kralj E, et al. Expression of fibronectin suicidal in gun shot wounds. Forensic Sci Int 2005; 17(147): 5-7.
  • Çelik S, Pakiş I, Yorulmaz Ç, Koç S. Immunhistochemical Study for Postmortem Diagnosis of Early Myocardial Infarction. p:28. 18th Triennial Meeting, New Orleans, Lousiana, USA July 21-25, 2008.
  • Dreßler J, Bachmann L, Strejc P, et al. Expression of adhesion molekules in skin wounds: diagnostic value in legal medicine. Forensic Sci Int 2000;113: 173-176.
  • Dreßler J, Bachmann L, Kasper M, et al. Enhanced expression of ICAM-1 (CD 54) in human skin wounds: diagnostic value in legal medicine. Inflamm Res 1997;46: 434-435.
  • Dreßler J, Bachmann L, Koch R, et al. Estimation of wound age and VCAM-1 in human skin, Int J Legal Med 1999;112: 159-162.
  • Dreßler J, Bachmann L, Koch R, et al. Enhanced expression of selectins in human skin wounds. Int J Legal Med 1998;112: 39–44.
  • Dreßler J, Bachmann L, Kasper M, et al. Time dependence of the expression of ICAM-1 (CD 54) in human skin wounds. Int J Legal Med 1997;110: 299–304.
  • Takamiya M, Fujita S, Saigusa K, et al. Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation. Int J Legal Med 2008;122(2): 143-8.
  • Takamiya M, Fujita S, Saigusa K, et al. Simultaneous detections of 27 cytokines during cerebral wound healing by multiplexed bead-based immunoassay for wound age estimation. J Neurotrauma 2007;24(12): 1833-44.
  • Grellner W. Time-dependent immunohistochemical detection of proinflammatory cytokines (IL-1beta, IL-6, TNF-alpha) in human skin wounds. Forensic Sci Int 2002: 4;130(2-3): 90-6.
  • Grellner W, Georg T, Wilske J. Quantitative analysis of proinflammatory cytokines (IL1beta, IL-6, TNF-alpha) in human skin wounds. Forensic Sci Int 2000: 11;113(1-3): 251-64.
  • Kondo T, Ohshima T, Eisenmenger W. Immunohistochemical and morphometrical study on the temporal expression of interleukin-1alpha (IL-1alpha) in human skin wounds for forensic wound age determination. Int J Legal Med 1999;112: 249–52.
  • Lin ZQ, Kondo T, Ishida Y, et al. Essential involvement of IL-6 in the skin wound-healing process as evidenced by delayed wound healing in IL-6-deficient mice. J Leukoc Biol 2003;73: 713–21.
  • Mori R, Kondo T, Ohshima T, et al. Accelerated wound healing in tumor necrosis factor receptor p55-deficient mice with reduced leukocyte infiltration. FASEB J 2002;16: 963–74.
  • Michel G, Kemeny L, Peter RU, et al. Interleukin-8 receptor-mediated chemotaxis of normal human epidermal cells. FEBS Lett 1992;305: 241–3.
  • Takamiya M, Saigusa K, Aoki Y. Immunohistochemical study of basic fibroblast growth factor and vascular endothelial growth factor expression for age determination of cutaneous wounds. Am J Forensic Med Pathol 2002;23(3): 264-7.
  • Hayashi T, Ishida Y, Kimura A, et al. Forensic application of VEGF expression to skin wound age determination. Int J Legal Med 2004;118: 320–5.
  • Gilbert L,Siang HL. Forensic histopathology chapter 13 part 8. In: Michael Tsokos, ed. Forensic pathology reviews, vol.5, 2008;240-64.
  • Grellner W, Vieler S, Madea B. Transforming growth factors (TGFalpha and TGF-beta1) in the determination of vitality and wound age: immunohistochemical study on human skin wounds. Forensic Sci Int 2005;153: 174– 80.
  • Ishida Y, Kondo T, Takayasu T, et al. The essential involvement of cross-talk between IFN-gamma and TGF-beta in the skin wound-healing process. J Immunol 2004: 1;172(3): 1848- 55.
  • Kondo T, Tanaka J, Ishida Y, et al. Ubiquitin expression in skin wounds and its application to forensic wound age determination. Int J Legal Med. 2002: 116(5): 267-72.
  • Quan L, Zhu BL, Oritani S, et al. Intranuclear ubiquitin immunoreactivity in the pigmented neurons of the substantia nigra in fire fatalities. Int J Legal Med 2001;114(6): 310-5.
  • Bond U, Schlesinger MJ. The chicken ubiquitin gene contains a heat shock promoter and expresses an unstable mRNA in heat-shocked cells. Mol Cell Biol 1986;6(12): 4602-10.
  • Quan L, Zhu BL, Ishida K, et al. Intranuclear ubiquitin immunoreactivity of the pigmented neurons of the substantia nigra in fatal acute mechanical asphyxiation and drowning. Int J Legal Med 2001;115(1): 6-11.
  • Ishida Y, Kimura A, Takayasu T, et al. Expression of oxygenregulated protein 150 (ORP150) in skin wound healing and its application for wound age determination. Int J Legal Med 2008;122(5): 409-14.
  • Ozawa K, Kondo T, Hori O, et al. Expression of the oxygen-regulated protein ORP150 accelerates wound healing by modulating intracellular VEGF transport. J Clin Invest 2001;108(1): 41-50.
  • Kuwabara K, Matsumoto M, Ikeda J, et al. Purification and characterization of a novel stress protein, the 150-kDa oxygen-regulated protein (ORP150), from cultured rat astrocytes and its expression in ischemic mouse brain. J Biol Chem 1996;271(9): 5025-32.
  • Hausmann R, Nerlich A, Betz P.The time-related expression of p53 protein in human skin wounds-a quantitative immunohistochemical analysis. Int J Legal Med 1998;111(4): 169-72
  • Sato Y, Ohshima T. The expression of mRNA of proinflammatory cytokines during skin wound healing in mice: a preliminary study for forensic wound age estimation (II). Int J Legal Med 2000;113(3): 140-5.
  • Takamiya M, Saigusa K, Nakayashiki N, et al. Studies on mRNA expression of basic fibroblast growth factor in wound healing for wound age determination. Int J Legal Med 2003;117(1): 46-50.
  • Takamiya M, Saigusa K, Kumagai R, et al. Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation. Int J Legal Med 2005;119(1): 16-21.
  • Kagawa S, Matsuo A, Yagi Y, et al. The time-course analysis of gene expression during wound healing in mouse skin. Leg Med (Tokyo) 2009;11(2): 70-75.
  • Sun JH, Wang YY, Zhang L, et al. Time-dependent expression of skeletal muscle troponin I mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation. Int J Legal Med 2010;124(1): 27- 33.
  • Takamiya M, Fujita S, Saigusa K, et al. A study on mRNA expressions of interleukin 10 during fracture healing for wound age determination. Leg Med (Tokyo) 2008;10(3): 131-7.
  • Betz P, Nerlich A, Tübel J, et al. Detection of cell death in human skin wounds of various ages by an in situ end labeling of nuclear DNA fragments. Int J Legal Med1997;110(5): 240-3.
Toplam 70 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Adli Biyoloji
Bölüm Araştırma Makalesi
Yazarlar

Işıl Pakiş Bu kişi benim

Evrim Akçakaya Bu kişi benim

Gönderilme Tarihi 20 Ağustos 2010
Yayımlanma Tarihi 31 Ağustos 2011
IZ https://izlik.org/JA88SA37SP
Yayımlandığı Sayı Yıl 2011 Cilt: 25 Sayı: 2

Kaynak Göster

Vancouver 1.Işıl Pakiş, Evrim Akçakaya. ADLİ TIP UYGULAMALARINDA YARA YAŞI VE CANLILIK BULGULARININ DEĞERLENDİRİLMESİ. ATD [Internet]. 01 Ağustos 2011;25(2):69-76. Erişim adresi: https://izlik.org/JA88SA37SP

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