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Evaluation of Biocompatibility Properties of Dental Materials: xCELLigence® System

Yıl 2024, Cilt: 13 Sayı: 2, 395 - 401, 20.05.2024
https://doi.org/10.54617/adoklinikbilimler.1401043

Öz

The toxic and biological impacts of dental materials play a pivotal role in their clinical application within dentistry. The assessment of these materials typically commences with in vitro tests upon initial development, progressing to in vivo animal experiments and clinical trials. In vitro cell culture tests afford the examination of tissue responses at the cellular level, allowing the observation of physiological activities. Moreover, these tests offer a cost-effective and time-efficient alternative to animal experiments, rendering them easily applicable and replicable. Recently, real-time cell analysis systems, such as the xCELLigence® system, have emerged as a promising substitute for traditional testing methods, potentially surpassing them in the biocompatibility evaluation of dental materials. The xCELLigence® system facilitates the concurrent observation and analysis of cells within their authentic environment, obviating the need for cell staining or marking. This review seeks to underscore the advantageous features of the xCELLigence® system, which serves to mitigate the drawbacks associated with conventional in vitro biocompatibility evaluation methods.

Kaynakça

  • Hanks C, Wataha J, Sun Z. In vitro models of biocompatibility: A review. Dent Mater 1996;12:186–93.
  • Tuncer S, Demirci M. Dental materyallerde biyouyumluluk değerlendirmeleri. Atatürk Üniv Diş Hek Fak Derg 2011;21:141–9.
  • Adabi M, Naghibzadeh M, Zarrinfard MA, Esnaashari SS, Seifalian AM, Faridi-Majidi R, et al. Biocompatibility and nanostructured materials: Applications in nanomedicine. Artif Cells, Nanomedicine Biotechnol 2017;45:833–42.
  • Schmalz G, Arenholt-Bindslev D. Biocompatibility of dental materials. Berlin: Springer; 2009.
  • Koçak S, Erten H. Mineral trioksit agregat’ın biyouyumluluğunun değerlendirilmesi. Acta Odontol Turc 2012;29:63–71.
  • Murray PE, Garcia Godoy C, Garcia Godoy F. How is the biocompatibility of dental materials evaluated? Med Oral Patol Cir Bucal 2007;12:E258-66.
  • International Organization for Standardization. Dentistry - Evaluation of biocompatibility of medical devices used in dentistry. Test methods for dental materials. ISO 7405; 2008.
  • International Organization for Standardization. Dentistry- Biological evaluation of medical devices. Tests for in vitro cytotoxicity. ISO 10993-5; 2009.
  • Uzun İ, Bayındır F. Dental materyallerin biyouyumluluk test yöntemleri. Gazi Üniversitesi Diş Hekim Fakültesi Derg 2011;28:115–22.
  • Wataha JC. Principles of biocompability for dental practioners. J Prosthet Dent 2001;86:203–9.
  • Pizzoferrato A, Ciapetti G, Stea S, Cenni E, Arciola C GD. Cell culture methods for testing biocompability. Clin Mater 1994;15:173–90.
  • Schmalz G. Use of cell cultures for toxicity testing of dental materials-advantages and limitations. J Dent 1994;22:6–11.
  • Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clin Oral Investig 1997;1:154–62.
  • Helgason C, Miller C. Methods in moleculer biology. Third Edit. Tatowa: Humana Press; 2005. p.1–12.
  • Tokur O, Aksoy A. In Vitro Sitotoksisite Testleri. Harran Üniv Vet Fak Derg 2017;6:112–8.
  • Powers J, Sakaguchi R. Craig’s restorative dental materials. 12th ed. St. Louis: Mosby Elsevier; 2006. p.97–125.
  • Yıldırım ZS, Bakır EP, Bakır Ş, Aydın MS. Diş hekimliğinde biyouyumluluk ve değerlendirme yöntemleri. Selcuk Dent J 2017;4:162–9.
  • Saw TY, Cao T, Yap AUJ, Ng MML. Tooth slice organ culture and established cell line culture models for cytotoxicity assesment of dental materials. Toxicol Vitr 2005;19:145–54.
  • Mallineni SK, Nuvvula S, Matinlinna JP, Yiu CK, King NM. Biocompatibility of various dental materials in comtemporary dentistry: a narrative insight. J Investig Clin Dent 2013;4:9–19.
  • Galluzzi L ve ark. Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. Cell Death Differ 2009;16:1093–107.
  • Polyzois GL. In vitro evaluation of dental materials. Clin Mater 1994;16:21–60.
  • Moharamzadeh K, Brook IM NR. Biocompatibility of Resin-based Dental Materials. Materials 2009;2:514–48.
  • Wennberg A, Hasselgren G TL. A method for toxicity screening of biomaterials using cells cultured on Millipore filters. J Biomed Mater Res 1979;13:109–20.
  • Outhwaite WC, McKenzie DM PD. A versatile split-chamber device for studying dentin permeability. J Dent Res 1974;53:1503.
  • Schmalz G, Hiller K, Nunez L, Stoll J, Weis K. Permeability characteristics of bovine and human dentin under different pretreatment conditions. J Endod 2001;27:23–30.
  • Zorba Y, Yıldız M. Adeziv Restoratif Materyallerde Biyouyumluluk Testleri ve Kriterleri. Atatürk Üniv Diş Hek Fak Derg 2007;2:15–21.
  • Adan A, Kiraz Y, Baran Y. Cell Proliferation and Cytotoxicity Assays. Curr Pharm Biotechnol 2016;17:1213–21. Ke N, Wang X, Xu X AY. The xCELLigence system for real-time and label-free monitoring of cell viability. Methods Mol Biol 2011;740:33–43.
  • Erol S, Öztürk Kurt B, Dinç B, Özdemir S. HEK 293 hücrelerine farklı doz ve sürelerde uygulanan klorpirifos ve tannik asitin hücre canlılığı üzerine etkilerinin araştırılması. Sağlık Bilim İleri Araştırmalar Derg 2023;6:56–63.
  • Atilan Yavuz S, Sürmelioğlu D. Evaluation of Cytotoxicity of Different Universal Bonds Using the Xcelligence System. Cumhur Dent J 2020;23:371–81.
  • xCELLigence RTCA DP Real Time Cell Analyzer – Dual Purpose. https://www.agilent.com/en/product/cell-analysis/real-time-cell-analysis/rtca-analyzers

Dental Materyallerin Biyouyumluluk Özelliklerinin Değerlendirilmesi: xCELLigence® Sistemi

Yıl 2024, Cilt: 13 Sayı: 2, 395 - 401, 20.05.2024
https://doi.org/10.54617/adoklinikbilimler.1401043

Öz

Diş hekimliğinde dental materyallerin toksik ve biyolojik etkileri klinik kullanımda büyük bir öneme sahiptir. Dental materyaller yeni geliştirildiğinde canlı dokulardaki etkisi, etik ve yasal yükümlülükler nedeniyle öncelikle in vitro testler sonrasında in vivo hayvan deneyleri ve klinik deneyler ile değerlendirilmektedir. İn vitro hücre kültürü testleri ile dokuların hücre düzeyinde yanıtları incelenebilmekte ve fizyolojik aktiviteleri taklit edilebilmektedir. Ayrıca hücre kültürü testlerinin hayvan deneylerine göre maliyeti daha düşüktür. Daha kısa süre almakta, kolaylıkla uygulanabilmekte ve tekrar edilebilmektedir. Ancak gelişen ve değişen teknolojiyle birlikte geleneksel test yöntemlerine bir alternatif olan gerçek zamanlı hücre analiz sistemleri (xCELLigence® sistemi), dental materyallerin biyouyumluluk değerlendirmelerinde tercih edilebilir. Bu sistem ile hücrelerin boyanmasına veya işaretlenmesine gerek duyulmadan, hücreleri kendi gerçek ortamlarında gerçeğe eş zamanlı olarak gözlemlemek ve analiz etmek mümkündür. Bu derleme geleneksel in vitro biyouyumluluk değerlendirme yöntemlerinin dezavantajlarını elimine eden xCELLigence® sisteminin üstün özelliklerini vurgulamayı amaçlamaktadır.

Kaynakça

  • Hanks C, Wataha J, Sun Z. In vitro models of biocompatibility: A review. Dent Mater 1996;12:186–93.
  • Tuncer S, Demirci M. Dental materyallerde biyouyumluluk değerlendirmeleri. Atatürk Üniv Diş Hek Fak Derg 2011;21:141–9.
  • Adabi M, Naghibzadeh M, Zarrinfard MA, Esnaashari SS, Seifalian AM, Faridi-Majidi R, et al. Biocompatibility and nanostructured materials: Applications in nanomedicine. Artif Cells, Nanomedicine Biotechnol 2017;45:833–42.
  • Schmalz G, Arenholt-Bindslev D. Biocompatibility of dental materials. Berlin: Springer; 2009.
  • Koçak S, Erten H. Mineral trioksit agregat’ın biyouyumluluğunun değerlendirilmesi. Acta Odontol Turc 2012;29:63–71.
  • Murray PE, Garcia Godoy C, Garcia Godoy F. How is the biocompatibility of dental materials evaluated? Med Oral Patol Cir Bucal 2007;12:E258-66.
  • International Organization for Standardization. Dentistry - Evaluation of biocompatibility of medical devices used in dentistry. Test methods for dental materials. ISO 7405; 2008.
  • International Organization for Standardization. Dentistry- Biological evaluation of medical devices. Tests for in vitro cytotoxicity. ISO 10993-5; 2009.
  • Uzun İ, Bayındır F. Dental materyallerin biyouyumluluk test yöntemleri. Gazi Üniversitesi Diş Hekim Fakültesi Derg 2011;28:115–22.
  • Wataha JC. Principles of biocompability for dental practioners. J Prosthet Dent 2001;86:203–9.
  • Pizzoferrato A, Ciapetti G, Stea S, Cenni E, Arciola C GD. Cell culture methods for testing biocompability. Clin Mater 1994;15:173–90.
  • Schmalz G. Use of cell cultures for toxicity testing of dental materials-advantages and limitations. J Dent 1994;22:6–11.
  • Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clin Oral Investig 1997;1:154–62.
  • Helgason C, Miller C. Methods in moleculer biology. Third Edit. Tatowa: Humana Press; 2005. p.1–12.
  • Tokur O, Aksoy A. In Vitro Sitotoksisite Testleri. Harran Üniv Vet Fak Derg 2017;6:112–8.
  • Powers J, Sakaguchi R. Craig’s restorative dental materials. 12th ed. St. Louis: Mosby Elsevier; 2006. p.97–125.
  • Yıldırım ZS, Bakır EP, Bakır Ş, Aydın MS. Diş hekimliğinde biyouyumluluk ve değerlendirme yöntemleri. Selcuk Dent J 2017;4:162–9.
  • Saw TY, Cao T, Yap AUJ, Ng MML. Tooth slice organ culture and established cell line culture models for cytotoxicity assesment of dental materials. Toxicol Vitr 2005;19:145–54.
  • Mallineni SK, Nuvvula S, Matinlinna JP, Yiu CK, King NM. Biocompatibility of various dental materials in comtemporary dentistry: a narrative insight. J Investig Clin Dent 2013;4:9–19.
  • Galluzzi L ve ark. Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. Cell Death Differ 2009;16:1093–107.
  • Polyzois GL. In vitro evaluation of dental materials. Clin Mater 1994;16:21–60.
  • Moharamzadeh K, Brook IM NR. Biocompatibility of Resin-based Dental Materials. Materials 2009;2:514–48.
  • Wennberg A, Hasselgren G TL. A method for toxicity screening of biomaterials using cells cultured on Millipore filters. J Biomed Mater Res 1979;13:109–20.
  • Outhwaite WC, McKenzie DM PD. A versatile split-chamber device for studying dentin permeability. J Dent Res 1974;53:1503.
  • Schmalz G, Hiller K, Nunez L, Stoll J, Weis K. Permeability characteristics of bovine and human dentin under different pretreatment conditions. J Endod 2001;27:23–30.
  • Zorba Y, Yıldız M. Adeziv Restoratif Materyallerde Biyouyumluluk Testleri ve Kriterleri. Atatürk Üniv Diş Hek Fak Derg 2007;2:15–21.
  • Adan A, Kiraz Y, Baran Y. Cell Proliferation and Cytotoxicity Assays. Curr Pharm Biotechnol 2016;17:1213–21. Ke N, Wang X, Xu X AY. The xCELLigence system for real-time and label-free monitoring of cell viability. Methods Mol Biol 2011;740:33–43.
  • Erol S, Öztürk Kurt B, Dinç B, Özdemir S. HEK 293 hücrelerine farklı doz ve sürelerde uygulanan klorpirifos ve tannik asitin hücre canlılığı üzerine etkilerinin araştırılması. Sağlık Bilim İleri Araştırmalar Derg 2023;6:56–63.
  • Atilan Yavuz S, Sürmelioğlu D. Evaluation of Cytotoxicity of Different Universal Bonds Using the Xcelligence System. Cumhur Dent J 2020;23:371–81.
  • xCELLigence RTCA DP Real Time Cell Analyzer – Dual Purpose. https://www.agilent.com/en/product/cell-analysis/real-time-cell-analysis/rtca-analyzers
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Diş Terapötikleri, Farmakoloji ve Toksikoloji
Bölüm Derleme
Yazarlar

Makbule Buse Dundar Sarı 0000-0002-8848-8850

Yayımlanma Tarihi 20 Mayıs 2024
Gönderilme Tarihi 6 Aralık 2023
Kabul Tarihi 2 Ocak 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 13 Sayı: 2

Kaynak Göster

Vancouver Dundar Sarı MB. Evaluation of Biocompatibility Properties of Dental Materials: xCELLigence® System. ADO Klinik Bilimler Dergisi. 2024;13(2):395-401.