Effect of Vitamin C on Oxidant and Antioxidant Parameters in Cattle Immunized by Blackleg Vaccine

Yıl 2023, Cilt: 12 Sayı: 2, 7 - 12, 26.12.2023

Ertan Doğan , Oğuz Merhan , Hale Ergin Eğritağ , Kadir Bozukluhan , Dinçer Erdağ

https://doi.org/10.53913/aduveterinary.1307606

Öz

The goal of this study is to determine the effect of vitamin c on certain oxidant and antioxidant parameters in cattle immunized against blackleg. In the study, a total of 40 cattle aged 12 to 18 months, of different breeds and genders, were used in the Ardahan region. The cattle were divided into four equal groups. The Control group received 2 ml of saline, the Vitamin group received 5 mg/kg dose of vitamin c, the Vaccine-Vitamin group received 2 ml of blackleg vaccine and 5mg/kg vitamin c, and the Vaccine group received 2 ml of blackleg vaccine. Blood samples were taken from all animals before vaccination (day 0) and on the 2nd, 14th, and 28th days of administration. On the 2nd, 14th, and 28th days, malondialdehyde (MDA) and nitric oxide (NO) levels were substantially increased (P<0.05) in the Vaccine group compared to the Control and Vitamin groups, while superoxide dismutase (SOD) and catalase (CAT) levels were considerably decreased (P<0.05). It was noted that the amount of MDA and NO measured in the 2nd, 14th, and 28th days in the Vaccine-Vitamin group reduced compared to the Vaccine group, while the amount of SOD and CAT enhanced. The study concluded that the blackleg vaccine administered to cattle causes oxidative stress and lipid peroxidation, and that vitamin c acts as an antioxidant. Vitamin c administration with blackleg vaccine in cattle is believed to be beneficial for lowering oxidative stress.

Anahtar Kelimeler

Blackleg vaccine, Cattle, Lipid Peroxidation, Oxidative stress, Vitamin C

Yanıkara Aşısı ile Aşılanan Sığırlarda C Vitamininin Oksidan ve Antioksidan Parametreler Üzerine Etkisi

Öz

Bu araştırmanın amacı Yanıkara aşısı ile aşılanan sığırlarda c vitamininin bazı oksidan ve antioksidan parametreler üzerine etkisini belirlemektir. Araştırmada, Ardahan yöresinde ırk ve cinsiyetleri farklı, 12-18 aylık toplam 40 sığır kullanıldı. Sığırlar dört eşit gruba ayrıldı. Kontrol grubuna 2 ml serum fizyolojik, Vitamin grubuna 5 mg/kg dozda c vitamini, Aşı-Vitamin grubuna 2 ml yanıkara aşısı ve 5mg/kg c vitamini, Aşı grubuna ise 2 ml yanıkara aşısı enjekte edildi. Aşı ve ilaç uygulaması öncesinde (0.gün) ve uygulamanın 2. 14. ve 28. gününde tüm hayvanlardan kan örnekleri alındı. Uygulamanın 2.14. ve 28. gününde Aşı grubunda malondialdehit (MDA) ve nitrik oksit (NO) düzeyinin Kontrol ve Vitamin grubuna göre anlamlı oranda (P<0.05) arttığı, süperoksit dismutaz (SOD) ve katalaz (CAT) düzeylerinin ise anlamlı oranda (P<0.05) azaldığı görüldü. Aşı-Vitamin grubunda 2.14. ve 28. gün ölçülen MDA ve NO miktarının Aşı grubuna göre düştüğü, SOD ve CAT miktarının ise yükseldiği görüldü. Bu araştırmada, sığırlara uygulanan yanıkara aşısının oksidatif strese ve lipid peroksidasyonuna neden olduğu ve c vitamininin antioksidan etki gösterdiği sonucuna varıldı. Sığırlarda yanıkara aşısı ile birlikte c vitamini uygulamasının oksidatif stresi azaltma açısından faydalı olacağı düşünülmektedir.

Anahtar Kelimeler

C vitamini, Lipid Peroksidasyonu, Oksidatif stres, Sığır, Yanıkara aşısı

Kaynakça

• Abd Ellah, M.R., Okada, K., Goryo, M., Oishi, A., & Yasuda, J. (2009). Superoxide dismutase activity as a measure of hepatic oxidative stress in cattle following ethionine administration. The Veterinary Journal, 182 (2), 336–341. https://doi.org/10.1016/j. tvjl.2008.05.013
• Abreu, C.C., Edwards, E.E., Edwards, J.F., Gibbons, P.M., Leal de Araújo, J., Rech, R.R., & Uzal, F.A. (2017). Blackleg in cattle: A case report of fetal infection and a literature review. Journal of Veterinary Diagnostic Investigation, 29 (5), 612–621. https://doi. org/10.1177/1040638717713796
• Ali, A.F., Selim, A., Manaa, E.A., Abdelrahman, A., & Sakr, A. (2019). Oxidative state markers and clinicopathological findings associated with bovine leukemia virus infection in cattle. Microbial Pathogenesis, 136, 103662. https://doi.org/10.1016/j. micpath.2019.103662
• Araujo, R.F., Curci, V.C.L.M., Nobrega, F.L.C., Ferreira, R.M.M., & Dutra, I.S. (2010). Vaccination protocol and bacterial strain affect the serological response of beef calves against blackleg. Pesquisa Veterinaria Brasileira, 30 (7), 554–558. https://doi.org/10.1590/ S0100-736X2010000700008
• Arslan, H.H., Tarhan, D., Cenesiz, S., Alkan, F.A., Ozcan, U., Arslan, E.T., Barutcu, U.B., & Or, M.E. (2018). Evaluation of trace element levels and antioxidant metabolism in cattle with cutaneous papillomatosis. Acta Scientiae Veterinariae, 46 (1), 1–5. https://doi. org/10.22456/1679-9216.82553
• Aslankoç, R., Demirci, D., İnan, Ü., Yıldız, M., Öztürk, A., Çetin, M., Savran, E.Ş., & Yılmaz, B. (2019). Oksidatif stres durumunda antioksidan enzimlerin rolü - Süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GPX). SDÜ Tıp Fakültesi Dergisi, 26 (3), 362–369. https://doi.org/10.17343/sdutfd.566969
• Atakisi, E., & Merhan, O. (2017). Nitric oxide synthase and nitric oxide involvement in different toxicities, In Nitric Oxide Synthase - Simple Enzyme-Complex Roles (pp. 197–214). https://doi.org/10.5772/ intechopen.68494
• Atakisi, O., Oral, H., Atakisi, E., Merhan, O., Metin Pancarci, S., Ozcan, A., Marasli, S., Polat, B., Colak, A., & Kaya, S. (2010). Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Research in Veterinary Science, 89 (1), 10–13. https://doi.org/10.1016/j.rvsc.2010.01.008
• Bahrami, S., Esmaeilzadeh, S., & Oryan, A. (2014). Role of oxidative stress in concomitant occurrence of Fasciola gigantica and leiomyoma in cattle. Veterinary Parasitology, 203 (1–2), 43–50. https://doi.org/10.1016/j.vetpar.2014.02.047
• Bayyit, E., & Merhan, O. (2020). Some acute phase proteins and oxidative stress level in cows with normal parturition and dystocia. Ataturk Universitesi Veteriner Bilimleri Dergisi, 15 (2), 145–150https://doi.org/10.17094/ataunivbd.688400
• Bozukluhan, K., Merhan, O., Ögün, M., Cihan, M., & Gökçe, G. (2016). Omfalitisli buzağılarda bazı oksidatif stres parametre düzeylerinin belirlenmesi. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 30 (2), 79–81.
• Bozukluhan, K., Atakişi, E., & Atakişi, O. (2013). Nitric oxide levels, total antioxidant and oxidant capacity in cattle with foot-and-mouth- disease. Kafkas Universitesi Veteriner Fakultesi Dergisi, 19 (1), 179– 181. https://doi.org/10.9775/kvfd.2012.7244
• Bozukluhan, K., Merhan, O., Özcan, A., Gökçe, H.İ., & Gökçe, G. (2017). Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesi. Ankara Universitesi Veteriner Fakultesi Dergisi, 64 (2), 75–79. https://doi.org/10.1501/Vetfak_0000002778
• Chirase, N.K., Greene, L.W., Purdy, C.W., Loan, R.W., Auvermann, B.W., Parker, D.B., Walborg, E.F., Stevenson, D.E., Xu, Y., & Klaunig, J.E. (2004). Effect of transport stress on respiratory disease, serum antioxidant status, and serum concentrations of lipid peroxidation biomarkers in beef cattle. American Journal of Veterinary Research, 65 (6), 860–864. https://doi.org/10.2460/ajvr.2004.65.860
• Deǧer, S., Deǧer, Y., Ertekin, A., Gül, A., Biçek, K., & Özdal, N. (2008). Dictyocaulus viviparus ile enfekte sığırlarda lipit peroksidasyon ve antioksidan durumunun saptanmasi. Türkiye Parazitolojii Dergisi, 32 (3), 234–237.
• Deveci, H.A., & Güven, A. (2008). Investigation of blood MDA and GSH levels in cow with mastitis. Kafkas Universitesi Veteriner Fakultesi Dergisi, 14 (1), 63–66. https://doi.org/10.9775/kvfd.2008.05-a
• Doğan, E., Merhan, O., Erdağ, D., Karamancı, E., Bozukluhan, K., & Doğan, A.N.C. (2021). The effect of vitamin c on oxidant and antioxidant parameters in anthrax vaccine administered cattle. Van Veterinary Journal, 32 (3), 109–113. https://doi.org/10.36483/ vanvetj.958358
• Esmaeilnejad, B., Tavassoli, M., Samiei, A., Hajipour, N., Imani-Baran, A., & Farhang-Pajuh, F. (2018). Evaluation of oxidative stress and antioxidant status, serum trace mineral levels and cholinesterases activity in cattle infected with Anaplasma marginale. Microbial Pathogenesis, 123, 402–409. https://doi.org/10.1016/j. micpath.2018.07.039
• Frey, J., & Falquet, L. (2015). Patho-genetics of Clostridium chauvoei. Research in Microbiology, 166 (4), 384–392. https://doi. org/10.1016/j.resmic.2014.10.013
• Glombowsky, P., Bottari, N.B., Klauck, V., Fávero, J.F., Soldá, N.M., Baldissera, M.D., Perin, G., Morsch, V.M.,
• Schetinger, M.R.C., Stefani, L.M., & Da Silva, A.S. (2017). Oxidative stress in dairy cows seropositives for Neospora caninum. Comparative Immunology, Microbiology and Infectious Diseases, 54, 34–37. https://doi. org/10.1016/j.cimid.2017.07.007
• Heidarpour, M., Mohri, M., Borji, H., & Moghdass, E. (2013). Oxidant/ antioxidant status in cattle with liver cystic echinococcosis. Veterinary Parasitology, 195 (1–2), 131–135. https://doi. org/10.1016/j.vetpar.2013.01.018
• Jaguezeski, A.M., Baldissera, M.D., Rhoden, L.A., Gomes, T.M.A., Mendes, R.E., Bottari, N.B., Morsch, V.M., Schetinger, M.R.C., Stefani, L.M., Giongo, J.L., Vaucher, R.A., & Da Silva, A.S. (2018). Listeria monocytogenes impairs enzymes of the phosphotransfer network and alters antioxidant/oxidant status in cattle brain structures. Microbial Pathogenesis, 124, 284–290. https://doi. org/10.1016/j.micpath.2018.08.044
• Khoshvaghti, A., Askari, A., Nazifi, S., & Ghane, M. (2014). Evaluation of some antioxidant enzymes in cattle infected with foot and mouth virus. Istanbul Universitesi Veteriner Fakultesi Dergisi, 40 (1), 70–75. https://doi.org/10.16988/iuvfd.98796
• Kızıl, Ö., & Keltek, Y. (2017). Tüberkülozisli sığırlarda antioksidan parametrelerdeki değişimler. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 31 (1), 1–4.
• Kırbaş, A., Kaman, R., Merhan, O., Bozukluhan, K., Değirmençay, Ş., & Eroğlu, M.S. (2021). Evaluation of acute phase response and oxidative damage in sheep naturally infected with Streptococcus pluranimalium using haptoglobin, nitric oxide, and malondialdehyde levels. Fırat University Veterinary Journal of Health Sciences, 35 (3), 145–150.
• Kızıl, M., Baydar, E., & Kızıl, Ö. (2011). Tayleriyozisli sığırlarda parametrelerdeki değişiklikler. Fırat Üniversitesi Sağlık BilimleriVeteriner Dergisi, 25 (2), 53–56.
• Kızıl, Ö., & Gül, Y. (2004). Şap aşısı uygulanan besi sığırlarında antioksidan vitaminlerin klinik ve bazı hematolojik parametreler ile antioksidan enzim ve lipit peroksidasyon düzeylerine etkileri. Fırat Üniversitesi Sağlık Bilimleri Dergisi, 18 (2), 97–106.
• Łuszczak, J., Ziaja-Sołtys, M., & Rzymowska, J. (2011). Anti-oxidant activity of superoxide dismutase and glutathione peroxidase enzymes in skeletal muscles from slaughter cattle infected with Taenia saginata. Experimental Parasitology, 128 (2), 163–165. https://doi.org/10.1016/j.exppara.2011.01.007
• Mamak,N.,Kale,M.,&Yapıcı,O.(2018).Sığıryetı̇ştı̇rıċıl̇ığ̇ıṅdekullanılan aşılar ve önemi̇. Göller Bölgesi Aylık Hakemli Ekonomi ve Kültür Dergisi, 5 (63), 61–66.
• Mavangira, V., & Sordillo, L.M. (2018). Role of lipid mediators in the regulation of oxidative stress and inflammatory responses in dairy cattle. Research in Veterinary Science, 116, 4–14. https://doi. org/10.1016/j.rvsc.2017.08.002
• Meral, Ö., Ercan, N., & Fidancı, U.R. (2017). Septisemili buzağılarda lipid peroksidasyon düzeyi ve antioksidan enzim aktiviteleri. Ankara Universitesi Veteriner Fakultesi Dergisi, 64 (3), 161–164. https://doi. org/10.1501/Vetfak_0000002793
• Merhan, O., Bozukluhan, K., & Gökçe, H.İ. (2017). Acute phase proteins and biochemical and oxidative stress parameters in Hypoderma spp. infested cattle. Journal of the Hellenic Veterinary Medical Society, 68 (4), 535–540. https://doi.org/10.12681/jhvms.16049
• Miranda, K.M., Espey, M.G., & Wink, D.A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5 (1), 62–71.
• Nicholson, P., Furrer, J., Hässig, M., Strauss, C., Heller, M., Braga-Lagache, S., & Frey, J. (2019). Production of neutralizing antibodies against the secreted Clostridium chauvoei toxin A (CctA) upon blackleg vaccination.
• Anaerobe, 56, 78–87. https://doi.org/10.1016/j. anaerobe.2019.02.011
• Perin, G., Fávero, J.F., Severo, D.R.T., Silva, A.D., Machado, G., Araújo, H.L., Lilenbaum, W., Morsch, V.M., Schetinger, M.R.C., Jordão, R.S., Stefani, L.M., Bottari, N.B., & Da Silva, A.S. (2017). Occurrence of oxidative stress in dairy cows seropositives for Brucella abortus. Microbial Pathogenesis, 110, 196–201. https://doi.org/10.1016/j. micpath.2017.06.043
• Saleh, M.A. (2009). Erythrocytic oxidative damage in crossbred cattle naturally infected with Babesia bigemina. Research in Veterinary Science, 86 (1), 43–48. https://doi.org/10.1016/j.rvsc.2008.05.005
• Tufan, A., & Çam, Y. (2008). Lipid peroxidation level and antioxidant enzyme activities in calves coccidiosis. Journal of Health Sciences, 17 (3), 131–136.
• Uzlu, E., Karapehlivan, M., Erdoğan, H.M., Kızıltepe, Ş., Erkılıç, E.E., Deveci, H.A., Gökçe, E., Kaya, İ., & Çitil, M. (2016). Serum and saliva sialic acid and oxidative stress parameters changes in bulls with foot and mouth disease. Kafkas Universitesi Veteriner Fakultesi Dergisi, 22 (3), 321–325. https://doi.org/10.9775/kvfd.2015.13114
• Yılmaz, S., & Issi, M. (2014). Malondialdehyde and total antioxidant levels and hematological parameters of beef cattle with coccidiosis. Van Veterinary Journal, 25 (2), 41–45.
• Yoshioka, T., Kawada, K., Shimada, T., & Mori, M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. American Journal of Obstetrics and Gynecology, 135(3), 372–376. https://doi. org/10.1016/0002-9378(79)90708-7 Vitamin C on Oxidant and Antioxidant Parameters in Cattle