Investigation of Mobilized Colistin Resistance Gene-1 in Poultry Pathogenic Escherichia coli

Yıl 2024, Cilt: 13 Sayı: 2, 1 - 8, 30.12.2024

Ecenur Yilmaz Süheyla Türkyılmaz

https://doi.org/10.53913/aduveterinary.1469963

Öz

This study aimed to determine the presence of mobilized colistin resistance gene 1 (mcr-1), which provides plasmid-mediated colistin resistance in avian pathogenic Escherichia coli (APEC) isolates, and to examine the antibiotic resistance profiles of colistin resistant isolates. In this research, 200 broiler liver samples suspected of colibacillosis were utilized as the material. Following the isolation of E. coli through classical conventional methods, identification and antibiotic susceptibility tests were conducted using an automated microbiology system (BD Phoenix 100TM, USA). The presence of the mcr-1 gene in isolates phenotypically determined as colistin resistant was investigated using polymerase chain reaction (PCR). Out of 156 (78%) E. coli isolates obtained from 200 broiler liver samples, 10 (6.4%) were phenotypically determined to be colistin resistant. It was found that 80% of colistin resistant E. coli isolates were resistant to levofloxacin, 70% to cefazolin and cefuroxime, 60% to ceftazidime, 50% to gentamicin and ceftriaxone, 40% to cefepime, 30% to ceftolozane-tazobactam, and 20% to piperacillin-tazobactam. All isolates were sensitive to amikacin, ertapenem, imipenem, meropenem, and resistant to ampicillin, amoxicillin-clavulanate, ampicillin-sulbactam, trimethoprim-sulfamethoxazole, and tigecycline, exhibiting multidrug resistance (MDR). All isolates that were found to be phenotypically resistant to colistin were found to carry the mcr-1 gene. These findings indicate that the mcr in APEC contributes to the rapid spread of plasmid-mediated resistance genes and the escalation of broad-spectrum antibiotic resistance. The detection of resistance to antibiotics used in human medicine may be poses a potential threat to public health. Future studies should be conducted with samples from different regions and include a diverse sample group to better understand this risk.

Anahtar Kelimeler

Avian pathogenic Escherichia coli, mobile colistin resistance gene, antibiotic resistance

Etik Beyan

Gerek bulunmamaktadır.

Destekleyen Kurum

Aydın Adnan Menderes University Scientific Research Projects Unit

Proje Numarası

ADU-BAP, VTF-23009

Teşekkür

This study was summarized by the first author’s Master of Science thesis. This study was supported by Aydın Adnan Menderes University Scientific Research Projects Unit (Project Number: ADU-BAP, VTF-23009).

Kaynakça

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