Abamectin Resistance, Synergist and Esterase Enzyme Relationship in Tetranychus urtice Koch (Acari: Tetranychidae) Populations Collected from Apple Orchards in Isparta Province

Yıl 2025, Cilt: 22 Sayı: 1, 35 - 41, 30.06.2025

Sibel Yorulmaz , Hüseyin Kaplan , Emir Öztürk

https://doi.org/10.25308/aduziraat.1544756

Öz

In this study, abamectin resistance, IBP synergistic effect rates and esterase enzyme activities were investigated in Tetranychus urticae Koch (Acari: Tetranychidae) populations collected from Isparta province during the apple production season of 2023. A total of 5 T. urticae populations, 1 each from Eğirdir (E1), Gelendost (E2), Senirkent (E3), Uluborlu (E4), and Yalvaç (E5) districts of Isparta province where apple production is intensive. Dry residue method was used to determine the abamectin resistance levels of the populations and for synergistic effect trials. In the experiments, 7 insecticide doses + 1 control group were used. Each group consisted of 3 replicates. In each replicate, 25 adult red spiders were used. Acaricides were applied as 2 ml on the leaf surface at 1 atm pressure using a spray tower. Dead-live counts of the populations were made after 24 hours. Abamectin resistance rates in T. urticae populations were determined by the ratio of the LC50 values of the field populations to the LC50 value of the sensitive population. As a result of the study, abamectin resistance rates of T. urticae in E1, E2, E3, E4 and E5 populations collected from apple orchards were 143.6, 119, 143, 143, 760.6, and 83.3 times, respectively. IBP synergistic effect ratios were 3.6, 3.8, 1.8, 2.0, and 3.1 times in E1, E2, E3, E4 and E5 populations, respectively. High esterase enzyme activities were determined in all T. urticae populations collected from apple orchards compared to the susceptible population. When the results are evaluated together, it is thought that the use of abamectin in the control of T. urticae in apple orchards should be suspended for a while and it would be appropriate to add active ingredients with different mechanisms of action to the rotation.

Anahtar Kelimeler

Abamectin , Tetranychus urticae , IBP , esterase

Isparta İli Elma Bahçelerinden Toplanan Tetranychus urtice Koch (Acari: Tetranychidae) Popülasyonlarında Abamectin Direnci, Sinerjist ve Esteraz Enzim İlişkisi

Öz

Bu çalışmada, 2023 yılı elma üretim sezonu boyunca Isparta ilinden toplanan Tetranychus urticae Koch (Acari: Tetranychidae) popülasyonlarında abamectin direnci, IBP sinerjistik etki oranları ve esteraz enzim aktiviteleri araştırılmıştır. Isparta ilinde yoğun olarak elma üretimi yapılan Eğirdir (E1), Gelendost (E2), Senirkent (E3), Uluborlu (E4) ve Yalvaç (E5) ilçelerinden birer popülasyon alınarak toplam 5 ayrı Tetranychus urticae popülasyonu oluşturulmuştur Popülasyonların abamectin direnç düzeylerinin belirlenmesi ve sinerjistik etki denemeleri için kuru rezidü yöntemi kullanılmıştır. Denemelerde 7 insektisit konsantrasyonu+1 kontrol grubu kullanılmıştır. Her grup 3 tekerrürden oluşmuştur. Her tekerrürde ise 25 adet ergin kırmızı örümcek kullanılmıştır. Akarisitler ilaçlama kulesi kullanılarak 1 atm basınçta yaprak yüzeyine 2ml olarak uygulanmıştır. Popülasyonlarda ölü-canlı sayımları 24 saat sonra yapılmıştır. Tetranychus urticae popülasyonlarında abamectin direnç oranları tarla popülasyonlarının LC50 değerlerinin hassas popülasyonun LC50 değerine oranlanması ile belirlenmiştir. Çalışma sonucunda T.urticae’nin elma bahçelerinden toplanan E1, E2, E3, E4 ve E5 popülasyonlarında abamectin direnç oranları sırasıyla 143.6, 119. 143. 760.6 ve 83.3 kat olarak belirlenmiştir. IBP sinerjistik etki oranları ise, E1, E2, E3, E4 ve E5 popülasyonlarında sırasıyla 3.6, 3.8, 1.8, 2.0 ve 3.1 kat olarak bulunmuştur. Elma bahçelerinden toplanan tüm T. urticae popülasyonlarında hassas popülasyona göre yüksek esteraz enzim aktiviteleri belirlenmiştir. Sonuçlar birlikte değerlendirildiğinde, elma bahçelerinde Tetranychus urticae mücadelesinde abamectin kullanımına bir süre ara verilmesi gerektiği ve rotasyona farklı etki mekanizmasına sahip etken maddelerin eklenmesinin doğru olacağı düşünülmektedir.

Anahtar Kelimeler

Abamectin , Tetranychus urticae , IBP , esteraz

Kaynakça

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