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Balık ve Balık Ürünlerinde Biyojen Aminlerin Varlığı

Yıl 2015, Cilt: 12 Sayı: 1, 139 - 145, 01.03.2015

Öz

Biyojen aminler hayvan, bitki ve mikroorganizmaların normal metabolizmasında rol alan düşük molekül ağırlıklı
organik bazlardır. Genellikle serbest aminoasitlerin mikrobiyal enzimlerle dekarboksilasyonu sonucu
oluşmaktadır. Bu bileşikler, insan ve hayvanlarda toksik etkiye sahip olabilmektedirler. Biyojen aminler,
olgunlaştırma veya fermantasyon işlemleri ile üretilen çeşitli gıdalarda ve içeceklerde bulunmaktadır. Biyojen
amin içeren gıdalar arasında balık ve balık ürünleri, et ürünleri, yumurta, peynir çeşitleri, fermente sebzeler ve
soya fasulyesi ürünleri, bira ve şarap yer almaktadır. Biyojenik aminler kimyasal olarak alifatik (putresin,
kadaverin, spermin, spermidin), aromatik (tiramin, feniletilamin) ve heterosiklik (histamin, triptamin) yapıda
olabilirler. Balık kasında en sık bulunan aminler histamin, kadaverin ve putresindir. Bu derlemede, biyojenik
aminlerin önemi ve bunların balık ve balık ürünlerinde oluşumu üzerinde durulmuştur.

Kaynakça

  • Alper, N., Temiz, A., 2001. Gıdalardaki biyojen aminler ve önemi. Türk Hij. Den. Biyol. Der., 58 (2): 71-80.
  • Al Bulushi I. , Poole S. , C. Deeth H. & A. Dykes G.,2009. Biogenic Amines in Fish: Roles in Intoxication, Spoilage, and Nitrosamine Formation—A Review, Critical Reviews in Food Science and Nutrition, 49:4, 369-377, Taylor and Francis Group, LLC, ISSN: 1040- 8398.
  • Anonymous, 1975. Scombroid poisoning - New York City. Morbid. Mortal. 24:342–347.
  • Anonim, 2012. http://www.diatek.com.tr/Makale- Yontem/Saglik/Balik-ve-Balik-Urunlerinde-Olusan- B i y o j e n i k - A m i n l e r _ 1 6 7 . h t m ( E r i ş i m t a r i h i 22.05.2012)
  • An, H., Lee, H., Jun, S.H., Kim, S.H., 1996. Histamine formation in fermented seafood products. Dept. Nutrition & Food Sci. Auburn Uni. Alabama.
  • Aras Hisar Ş., Hisar, O., Yanık, T., 2004. Balıklarda mikrobiyolojik, enzimatik ve kimyasal bozulmalar. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 35 (3-4): 261-265.
  • Askar, A., Treptow, H., 1986. Biogene amine in Lebensmitteln. Vorkommen, Bedeutung und Bestimmung, Eugen Ulmer GmbH and Co, Stuttgart, Germany.
  • Aygün, O., 2003. Biyojen aminler-süt ve süt ürünlerindeki varlığı ve önemi. Uludağ Üniv. J. Fac. Vet. Med., 22 (1- 2:3): 91-95.
  • Bakar, J., Yassoralipour, A., Abu bakar, F., Rahman, R. A., 2010. Biogenic amine changes in barramundi (Lates calcarifer) slices stored at 0C and 4C. Food Chemistry, 119:467-470.
  • Bardocz, S., Duguid, T.J, Brown, D.S., 1995. The importance of dietary polyamines in cell regeneration and growth. British Journal of Nutrition, 73 (6): 819- 28.
  • Ben-Gigirey, B., Vieites Baptisda de Sousa, J.M., Villa, T.G., Velazquez-Barros, J., 1999. Histamine and cadaverine production by bacteria isolated from fresh and frozen albacore (Thunnus alalunga). J. of Food Pro., 62 (8):933-939.
  • Beutling, D., 1994. Factors Influencing tyramine formation b y E n t e r o c o c c u s f a e c a l i s . A r c h i v f ü r Lebensmittelhygiene, 45 (6): 128-31.
  • Bover-Cid, S., Hugas, M., Izquierdo-Pulido, M., Vidal- Carou, M.C., 2000. Reduction of biogenic amine formation using a negative amino acid decarboxylase starter culture for fermentation of fuet sausages. J. Food Protect., 63 (2):237-243.
  • Busstra, M.C., Siezen, C.L.E., Grubben, M.J.A., 2003. Tissue Levels Of Fish Fatty Acids and Risk Of Colorectal Adenomas: A Case-Control Study (Netherlands). Cancer Causes Control.14: 269-276.
  • Cemek, M., Bulut, S., Konuk, M., Akkaya, L., Birdane, Y., Yılmaz, E., 2006. Eber ve Karamık göllerinden avlanan sazan (Cyprinus carpio) ve turna (Esox lucius) balıklarında depolama sıcaklığı ve süresinin biyojen amin oluşumuna etkisi. Gıda Teknolojileri Elektronik Dergisi, 1: 27-34.
  • Chamberlain, T., 2001. Histamine levels in longlined tuna in Fiji: A comporison of samples from two different body sites and effect of storage at different temperatures. S.Pac. J.Nat. Sci., 19: 30-34.
  • Çaklı, Ş., Kışla, D., 2003. Su ürünlerinde mikrobiyal kökenli bozulmalar ve önleme yöntemleri. Ege Üniversitesi Su Ürünleri Dergisi, 20, (1-2): 239-245.
  • Çolak, H., Aksu, H., 2002. Gıdalarda biyojen aminlerin varlığı ve amin oluşumunu etkileyen faktörler. YYÜ Vet. Fak. Derg., 13 (1-2):35-40.
  • Edmunds, W.J., Eitenmiller, R.R., 1975. Effect of storage time and temperature on histamine content and histidine decarboxylase activity of aquatic species. J. Food Sci. 40:516-519.
  • Ekici, K., Sağun, E., Sancak, Y.C., Sancak, H., Yörük, İ., İşleyici, Ö., 2011. Dondurulmuş olarak muhafaza edilen inci kefalinde (Chalcalburnus tarichi, Pallas 1811) biyojen amin oluşumu ve mikrobiyolojik değişimlerin belirlenmesi. Y.Y.U Vet. Fak. Der. 22(2): 93-99.
  • Erginkaya, Z., Var, I., 1989. Et ve et ürünlerinde biyojenik aminler. Gıda, 14 (3): 171-174.
  • Eitenmiller, R. R., De Souza, S., 1984. Enzymatic Mechanisms for Amine Formation in Fish, In Seafood Toxins, ed. E. R. Ragelis, American Chemical Society, Washington, DC.
  • Fletcher, G. C., Summers, G., Veghel, P.W.C., 1998. Levels of histamine and histamine-producing bacteria in smoked fish from New Zealand markets. J. of Food Pro., 61: 1064-1070.
  • FDA, 1996. Decomposition and histamine in raw frozen tuna, and mahi-mahi, canned tuna; and related species: Complince Policy Guides. 7108, 240, Sec. 540. 525.
  • Gökoğlu, N., Varlık, C., 1995. Sardalya konservelerinin histamin biyojen amini yönünden incelenmesi. Gıda, 5: 273-279.
  • Halasz, A., Barath, L.S.S., Holzapfel, W., 1994. Biogenic amines and their production by microorganisms in food. Trends in Food Science and Technology. 5: 42- 49.
  • Hernandez-Jover, T., Izquierdo-Pulido, M., Veciana- Nogues, M.T, Vidal-Carou, M.C., 1996. Ion-pair high performance liquid chromatographic determination of biogenic amines in meat and meat products. J Agric Food Chem., 44: 2710-2715.
  • Hernandez-Jover, T., Izquierdo-Pulido, M., Veciana- Nogues, M.T, Marine-Font, A., Vidal-Carou, M.C., 1997. Biogenic amine and polyamine contents in meat and meat products. J. Agric. Food Chem., 45 (6):2098- 2102.
  • Hwang, D., Chang, S., Shiau, C. and Cheng, C., 1995. Biogenic amines in the flesh of sailfish (Istiophorus platyterus) responsible for scombroid poisoning, J. Food Sci. 60:926–928.
  • Hornero-Mendez D., Garridio-Fernandez A., 1997. Rapid high performance liquid chromatography analysis of biogenic amines in fermented vegetable brines. Journal of Food Protection, 60 (4):414-419.
  • Izquierdo-Pulido, M., Hernandez-Jover, T., Marine-Font, A., Vidal-Carou, M.C., 1996. Biogenic amines in European beers. J Agric Food Chem., 44: 3159-3163.
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  • Pınar OĞUZHAN YILDIZ
  • pinaroguzhan@ardahan.edu.tr
  • Ardahan Üniversitesi Mühendislik Fakültesi
  • Gıda Mühendisliği Bölümü, ARDAHAN Geliş Tarihi Kabul Tarihi : 30.06.2015

Presence of Biogenic Amines in Fish and Fish Products

Yıl 2015, Cilt: 12 Sayı: 1, 139 - 145, 01.03.2015

Öz

Biogenic amines are low molecular organic bases in normal metabolisms of animal, vegetable and
microorganisms. These amines are formed by decarboxylation of free amino acids with microbial enzymes.
These compounds can have toxic efffects in human and animal. Biogenic amines, produced by ripening or
fermentation processes are in various foods and beverages. Biogenic amines such as fish, fish products, meat
products, eggs, cheese, fermented vegetables, soy bean products, beer and wine are available. The chemical
structure of bioljenik amines aliphatic (putrescine, cadaverine, spermine, spermidine), aromatic (tyramine,
phenylethylamine) and heterocyclic (histamine, tryptamin) are vary. Most commonly amines are histamine,
cadaverine and putresin in fish muscle. In this review, significance on the biogenic amines and the formation this
amines of in fish and fish products were examined.

Kaynakça

  • Alper, N., Temiz, A., 2001. Gıdalardaki biyojen aminler ve önemi. Türk Hij. Den. Biyol. Der., 58 (2): 71-80.
  • Al Bulushi I. , Poole S. , C. Deeth H. & A. Dykes G.,2009. Biogenic Amines in Fish: Roles in Intoxication, Spoilage, and Nitrosamine Formation—A Review, Critical Reviews in Food Science and Nutrition, 49:4, 369-377, Taylor and Francis Group, LLC, ISSN: 1040- 8398.
  • Anonymous, 1975. Scombroid poisoning - New York City. Morbid. Mortal. 24:342–347.
  • Anonim, 2012. http://www.diatek.com.tr/Makale- Yontem/Saglik/Balik-ve-Balik-Urunlerinde-Olusan- B i y o j e n i k - A m i n l e r _ 1 6 7 . h t m ( E r i ş i m t a r i h i 22.05.2012)
  • An, H., Lee, H., Jun, S.H., Kim, S.H., 1996. Histamine formation in fermented seafood products. Dept. Nutrition & Food Sci. Auburn Uni. Alabama.
  • Aras Hisar Ş., Hisar, O., Yanık, T., 2004. Balıklarda mikrobiyolojik, enzimatik ve kimyasal bozulmalar. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 35 (3-4): 261-265.
  • Askar, A., Treptow, H., 1986. Biogene amine in Lebensmitteln. Vorkommen, Bedeutung und Bestimmung, Eugen Ulmer GmbH and Co, Stuttgart, Germany.
  • Aygün, O., 2003. Biyojen aminler-süt ve süt ürünlerindeki varlığı ve önemi. Uludağ Üniv. J. Fac. Vet. Med., 22 (1- 2:3): 91-95.
  • Bakar, J., Yassoralipour, A., Abu bakar, F., Rahman, R. A., 2010. Biogenic amine changes in barramundi (Lates calcarifer) slices stored at 0C and 4C. Food Chemistry, 119:467-470.
  • Bardocz, S., Duguid, T.J, Brown, D.S., 1995. The importance of dietary polyamines in cell regeneration and growth. British Journal of Nutrition, 73 (6): 819- 28.
  • Ben-Gigirey, B., Vieites Baptisda de Sousa, J.M., Villa, T.G., Velazquez-Barros, J., 1999. Histamine and cadaverine production by bacteria isolated from fresh and frozen albacore (Thunnus alalunga). J. of Food Pro., 62 (8):933-939.
  • Beutling, D., 1994. Factors Influencing tyramine formation b y E n t e r o c o c c u s f a e c a l i s . A r c h i v f ü r Lebensmittelhygiene, 45 (6): 128-31.
  • Bover-Cid, S., Hugas, M., Izquierdo-Pulido, M., Vidal- Carou, M.C., 2000. Reduction of biogenic amine formation using a negative amino acid decarboxylase starter culture for fermentation of fuet sausages. J. Food Protect., 63 (2):237-243.
  • Busstra, M.C., Siezen, C.L.E., Grubben, M.J.A., 2003. Tissue Levels Of Fish Fatty Acids and Risk Of Colorectal Adenomas: A Case-Control Study (Netherlands). Cancer Causes Control.14: 269-276.
  • Cemek, M., Bulut, S., Konuk, M., Akkaya, L., Birdane, Y., Yılmaz, E., 2006. Eber ve Karamık göllerinden avlanan sazan (Cyprinus carpio) ve turna (Esox lucius) balıklarında depolama sıcaklığı ve süresinin biyojen amin oluşumuna etkisi. Gıda Teknolojileri Elektronik Dergisi, 1: 27-34.
  • Chamberlain, T., 2001. Histamine levels in longlined tuna in Fiji: A comporison of samples from two different body sites and effect of storage at different temperatures. S.Pac. J.Nat. Sci., 19: 30-34.
  • Çaklı, Ş., Kışla, D., 2003. Su ürünlerinde mikrobiyal kökenli bozulmalar ve önleme yöntemleri. Ege Üniversitesi Su Ürünleri Dergisi, 20, (1-2): 239-245.
  • Çolak, H., Aksu, H., 2002. Gıdalarda biyojen aminlerin varlığı ve amin oluşumunu etkileyen faktörler. YYÜ Vet. Fak. Derg., 13 (1-2):35-40.
  • Edmunds, W.J., Eitenmiller, R.R., 1975. Effect of storage time and temperature on histamine content and histidine decarboxylase activity of aquatic species. J. Food Sci. 40:516-519.
  • Ekici, K., Sağun, E., Sancak, Y.C., Sancak, H., Yörük, İ., İşleyici, Ö., 2011. Dondurulmuş olarak muhafaza edilen inci kefalinde (Chalcalburnus tarichi, Pallas 1811) biyojen amin oluşumu ve mikrobiyolojik değişimlerin belirlenmesi. Y.Y.U Vet. Fak. Der. 22(2): 93-99.
  • Erginkaya, Z., Var, I., 1989. Et ve et ürünlerinde biyojenik aminler. Gıda, 14 (3): 171-174.
  • Eitenmiller, R. R., De Souza, S., 1984. Enzymatic Mechanisms for Amine Formation in Fish, In Seafood Toxins, ed. E. R. Ragelis, American Chemical Society, Washington, DC.
  • Fletcher, G. C., Summers, G., Veghel, P.W.C., 1998. Levels of histamine and histamine-producing bacteria in smoked fish from New Zealand markets. J. of Food Pro., 61: 1064-1070.
  • FDA, 1996. Decomposition and histamine in raw frozen tuna, and mahi-mahi, canned tuna; and related species: Complince Policy Guides. 7108, 240, Sec. 540. 525.
  • Gökoğlu, N., Varlık, C., 1995. Sardalya konservelerinin histamin biyojen amini yönünden incelenmesi. Gıda, 5: 273-279.
  • Halasz, A., Barath, L.S.S., Holzapfel, W., 1994. Biogenic amines and their production by microorganisms in food. Trends in Food Science and Technology. 5: 42- 49.
  • Hernandez-Jover, T., Izquierdo-Pulido, M., Veciana- Nogues, M.T, Vidal-Carou, M.C., 1996. Ion-pair high performance liquid chromatographic determination of biogenic amines in meat and meat products. J Agric Food Chem., 44: 2710-2715.
  • Hernandez-Jover, T., Izquierdo-Pulido, M., Veciana- Nogues, M.T, Marine-Font, A., Vidal-Carou, M.C., 1997. Biogenic amine and polyamine contents in meat and meat products. J. Agric. Food Chem., 45 (6):2098- 2102.
  • Hwang, D., Chang, S., Shiau, C. and Cheng, C., 1995. Biogenic amines in the flesh of sailfish (Istiophorus platyterus) responsible for scombroid poisoning, J. Food Sci. 60:926–928.
  • Hornero-Mendez D., Garridio-Fernandez A., 1997. Rapid high performance liquid chromatography analysis of biogenic amines in fermented vegetable brines. Journal of Food Protection, 60 (4):414-419.
  • Izquierdo-Pulido, M., Hernandez-Jover, T., Marine-Font, A., Vidal-Carou, M.C., 1996. Biogenic amines in European beers. J Agric Food Chem., 44: 3159-3163.
  • Jay, J.M., 1992. Modern Food Microbiology. 4th edition, Chapman and Hall, New York .
  • Jones, J.M., 1992. Food safety. 2nd Print. Minnesota USA: Eagon press St. Paul.
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  • Kalac, P., Spicka, J., Krizek, M., Steidlova, S., Pelikanova, T., 1999. Concentrations of seven biogenic amines in sauerkraut. Food Chem., 67: 275-280.
  • Kamarı, N., 2007. Buzda depolanan yayın balığı (Siluris glanis L. 1758) nükleotid yıkım ve biyojenik amin konsantrasyonunun araştırılması. Çukurova Üniv. Fen Bilimleri Enstitüsü, Y. Lisans Tezi, Adana.
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  • Lehane, L., Olley, J., 2000. Histamine fish poisoning revisited. Int. J. of Food Mic., 58:1-37.
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  • Loser, C., 2000. Polyamines in human and animal milk. Br. J. Nutr., 84: Suppl. 1, S55-S58.
  • Maijala, R.L., Eerola, S.H., Aho, M.A., Hirn, J.A., 1993. The Effect of GDL-induced pH decrease on the formation of biogenic amines in meat. J. Food Protect., 56 (2): 125-129.
  • Maijala, R., 1994. Histamine and tyramine production by a Lactobacillus strain subjected to external pH decrease. J. Food Protect., 57 (3): 259-262.
  • Mitchell, J., 1993. Scombrotoxic fish poisoning. Report for the Ministry of Health, Wellington.
  • Mohamed, R., Livia, S.S., Hassan, S., Soher, E.S., Ahmed- Adel, E.B., 2009. Changes in free amino acids and biogenic amines of Egyptian salted-fermented fish (Feseekh) during ripening and storage. Food Chemistry, 115: 635-638.
  • Nakamura, M., Wada, Y., Sawaya, H., Kawabata, T., 1979. Polyamine content in fresh and processed pork. J. Food Sci., 44 (2): 515-517.
  • Ordonez, Al., Ibanez, F.C., Torre, P., Barcina, Y., 1997. Formation of biogenic amines in Idiazabal Ewe's-Milk cheese. Effect of ripening, pasteurization and starter. J. Food Protect. 60 (11):1371-1375.
  • Ölmez, H.K. 2000. Biyojenik aminler. Dünya Gıda, 51-57.
  • Özdestan, Ö., Üren, A., 2006. Biyojen amin analiz yöntemleri. Türkiye 9. Gıda Kongresi, 24-26 Mayıs, Bolu.
  • Özoğul, F., Küley, E., Özoğul, Y., 2004. Balık ve balık ürünlerinde oluşan biyojen aminler. E.Ü. Su Ürünleri Dergisi, 21 (3-4):375-381.
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  • Plahar , W.A., Nerquaye-Tetteh, G.A., Anan, N.T., 1999. Development of an Integrated Quality Assurance System for the Traditional Sardinella sp. and Anchovy Fish Smoking Industry in Ghana. Food Research Institue. Accra. Ghana. Food Control, 10:15-25.
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  • Ritchie, A. H., Mackie, I. M., 1980. The Formation of Diamines and Polyamines During Storage Of Mackerel (Scomber scombrus). In Advances in Fishery Science and Technology (Ed. Connell, J. J.) pp. 489-494. Farnham. Fishing News Books.
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  • Veciana-Nogues, M. T., Marine-Font,A., Vidal-Carou, M. C., 1997b. Biogenic amines as hygenic quality indicators of tunas. Relationships with microbial counts, ATP-related compounds, volatile amines, and organoleptik changes. J. Agric. Food Chem. 45: 2036- 2041.
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  • Yahia, D.A., Madani, S., Savelli, J.L,. 2003. Dietary Fish Protein Lowers Blood Pressure and Alters Tissue Polyunsaturated Fatty Acid Composition in Spontane ously Hypertensive Rats.Nutrition 19: 342-346.
  • Yassoralipour, A., Bakar, J., Abdul Rahman R., Abu Bakar, F., 2012. Biogenic amines formation in barramundi (Lates calcarifer) fillets at 8C kept in modified atmosphere packaging with varied CO2 concentration. LWT-Food Science and Technology, 48:142-146.
  • Yeğin, S., Üren, A., 2008. Gıdalarda biyojen amin oluşumunu etkileyen faktörler. Türkiye 10. Gıda Kongresi, 21-23 Mayıs, Erzurum.
  • Yerlikaya, P., Gökoğlu, N., 2002. Gıdalarda biyojen aminler ve önemi. Gıda Mühendisliği Dergisi, 6, 24-30.
  • Zhai, H., Yang, X., Li, L., Xia, G., Cen, J., Huang, H., Hao, S., 2012. Biogenic amines in commercial fish and fish products sold in southern China. Food Control, 25: 303-308.
  • Zotos, A., Hole, M., and Smith, G., 1995. The Effect of Frozen Storage of Mackarel (Scomber scombrus) on its Quality When Hot Smoked. Journal Science Food Agriculture, 67: 43-48. Sorumlu Yazar
  • Pınar OĞUZHAN YILDIZ
  • pinaroguzhan@ardahan.edu.tr
  • Ardahan Üniversitesi Mühendislik Fakültesi
  • Gıda Mühendisliği Bölümü, ARDAHAN Geliş Tarihi Kabul Tarihi : 30.06.2015
Toplam 86 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA83HV97CK
Bölüm Düzeltme
Yazarlar

Pınar Oğuzhan Yıldız Bu kişi benim

Birsen Kırım Bu kişi benim

Yayımlanma Tarihi 1 Mart 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 12 Sayı: 1

Kaynak Göster

APA Oğuzhan Yıldız, P., & Kırım, B. (2015). Balık ve Balık Ürünlerinde Biyojen Aminlerin Varlığı. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 12(1), 139-145.
AMA Oğuzhan Yıldız P, Kırım B. Balık ve Balık Ürünlerinde Biyojen Aminlerin Varlığı. ADÜ ZİRAAT DERG. Haziran 2015;12(1):139-145.
Chicago Oğuzhan Yıldız, Pınar, ve Birsen Kırım. “Balık Ve Balık Ürünlerinde Biyojen Aminlerin Varlığı”. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 12, sy. 1 (Haziran 2015): 139-45.
EndNote Oğuzhan Yıldız P, Kırım B (01 Haziran 2015) Balık ve Balık Ürünlerinde Biyojen Aminlerin Varlığı. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 12 1 139–145.
IEEE P. Oğuzhan Yıldız ve B. Kırım, “Balık ve Balık Ürünlerinde Biyojen Aminlerin Varlığı”, ADÜ ZİRAAT DERG, c. 12, sy. 1, ss. 139–145, 2015.
ISNAD Oğuzhan Yıldız, Pınar - Kırım, Birsen. “Balık Ve Balık Ürünlerinde Biyojen Aminlerin Varlığı”. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 12/1 (Haziran 2015), 139-145.
JAMA Oğuzhan Yıldız P, Kırım B. Balık ve Balık Ürünlerinde Biyojen Aminlerin Varlığı. ADÜ ZİRAAT DERG. 2015;12:139–145.
MLA Oğuzhan Yıldız, Pınar ve Birsen Kırım. “Balık Ve Balık Ürünlerinde Biyojen Aminlerin Varlığı”. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, c. 12, sy. 1, 2015, ss. 139-45.
Vancouver Oğuzhan Yıldız P, Kırım B. Balık ve Balık Ürünlerinde Biyojen Aminlerin Varlığı. ADÜ ZİRAAT DERG. 2015;12(1):139-45.