The detection of RT-qPCR method of Tospoviruses carried by Frankliniella occidentalis (Pergande) (Thysanoptera:Thripidae)

Yıl 2024, Cilt: 21 Sayı: 2, 187 - 193, 31.12.2024

Murat Kemal Avcı , Ferhat Kiremit , Eyyüp Mennan Yıldırım

https://doi.org/10.25308/aduziraat.1523822

Öz

Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) is a polyphagous species and can cause damage to many agricultural products such as cotton, strawberries, tomato and ornamental plants. F. occidentalis sucks the sap from the flowers of plants and causes premature flower fall and low yield. In addition, it can cause indirect damage because it is a virus vector. In this study, it was aimed to perform molecular identification of thrips obtained from the flowers of strawberry plants, which have economic importance for Aydın province, by applying PCR-RFLP protocol and to detect the presence of tospoviruses by the RT-qPCR method by obtaining RNA isolates. As a result of the study; it was determined that all species obtained were F. occidentalis. In addition, the presence of tospoviruses transmitted by these insects was also detected. Both methods seem promising to be used in future studies in terms of providing fast and reliable results.

Anahtar Kelimeler

Frankliniella occidentalis, RFLP, Tospovirus, RT-qPCR

Proje Numarası

ADÜ BAP ZRF-16010

Frankliniella occidentalis (Pergande) (Thysanoptera:Thripidae) tarafından taşınan Tospovirüslerin Moleküler Tespitinde RT-qPCR yönteminin uygulanması

Öz

Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) polifag bir tür olup, pamuk, çilek, domates ve süs bitkileri gibi birçok tarımsal üründe zararlara yol açabilmektedir. F. occidentalis bitkilerin çiçeklerindeki özsuyunu emmek suretiyle çiçeklerin erken dökülmesine ve verim düşüklüğüne neden olmaktadır. Ayrıca virüs vektörü olması nedeniyle bitkilerde dolaylı yoldan da zarar yapabilmektedir. Bu çalışmanın amacı, Aydın ili için ekonomik öneme sahip çileklerden elde edilen tripslerde vektörü oldukları tospovirüslerin varlığının RT-qPCR yöntemiyle tespit edilmesidir. Bu amaçla ilk olarak çilek bitkilerinin çiçeklerinden elde edilen tripslerin PCR-RFLP protokolüyle moleküler tanılaması yapıldıktan sonra RNA izolatları elde edilerek RT-qPCR yöntemiyle tospovirüslerin varlığı tespit edilmiştir. Çalışma sonucunda; elde edilen verilerle, eldeki örneklerin F. occidentalis olduğu ve bu böceklerle taşınan virüslerin de tospovirüs olduğu tespit edilmiştir. Her iki yöntem (PCR-RFLP ve RT-qPCR) hızlı ve güvenilir sonuç vermesi açısından, hem trips tespiti hem de tospovirüs tespiti amacıyla önümüzdeki çalışmalarda kullanılmak için ümitvar görünmektedir.

Anahtar Kelimeler

Frankliniella occidentalis, RFLP, Tospovirüs, RT-qPCR

Destekleyen Kurum

ADÜ BAP

Proje Numarası

ADÜ BAP ZRF-16010

Kaynakça

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