Investigation of Different Parts of Tea (Camellia sinensis (L.) O. Kuntze) in Terms of Polyphenol and Bioactivity

Yıl 2024, Cilt: 21 Sayı: 2, 245 - 250, 31.12.2024

Oğuzhan Civelekoğlu , Emine Yurteri

https://doi.org/10.25308/aduziraat.1565056

Öz

In this study, changes in the amount of polyphenols (C, EC, GC, EGC, EGCG, ECG) and caffeine in different parts (root, stem, leaf and apical bud) of tea plants harvested from gardens with different characteristics in three shoot periods were investigated. Analyses were performed by High-performance liquid chromatography (commonly known as HPLC). The highest amount of polyphenols were obtained from the leaf, apical bud, stem and root parts of the plant, respectively. In this study, the amount of polyphenols was generally found to be higher in the apical bud and leaf parts than in the root and stem. Polyphenols found in the apical bud and leaf of the tea plant, respectively; the highest values of EGCG were between 70.21-73.98 mg/g, caffeine 60.98-39.63 mg/g, EGC 23.83-31.43 mg/g, EC 15.04-15.86 mg/g, GC 6.95-10.92 mg/g and C 1.98-0.58 mg/g. The root and stem contain caffeine in low amounts. Polyphenols were found in the root and stem parts although less compared to the other two organs.

Anahtar Kelimeler

Tea, polyphenol, Catechin, HPLC

Çay (Camellia Sinensis (L.) O. Kuntze) Bitkisinin Farklı Kısımlarının Polifenol ve Biyoaktivite Yönünden Araştırılması

Öz

Bu çalışmada, farklı özellikteki bahçelerden üç sürgün döneminde hasat edilen çay bitkisinin farklı kısımlarında (kök, sap, yaprak ve tepe tomurcuğu) bulunan polifenol (C, EC, GC, EGC, EGCG, ECG) ve kafein miktarındaki değişimler incelenmiştir. Analizler Yüksek performanslı sıvı kromatografisi (HPLC yaygın adıyla bilinir) ile yapılmıştır. En yüksek polifenol miktarı sırasıyla bitkinin yaprak, tepe tomurcuğu, sap ve kök kısmından elde edilmiştir. Bu çalışmada polifenol miktarı tep tomurcuğu ve yaprak kısmında kök ve gövdeye göre genel olarak daha yüksek bulunmuştur. Çay bitkisinin tepe tomurcuğunda ve yaprağında sırasıyla bulunan polifenoller; en yüksek EGCG’ın 70.21-73.98 mg/g, Kafein’in 60.98-39.63 mg/g, EGC’in 23.83-31.43 mg/g, EC’in 15.04-15.86 mg/g, GC’in 6.95 -10.92 mg/g ve C’in 1.98-0.58 mg/g değerleri arasında olmuştur. Kök ve sap çok fazla olmamakla beraber kafein içermektedir. Kök ve sap kısmında diğer iki organa göre kıyasla az da olsa polifenol bulunmuştur.

Anahtar Kelimeler

ÇAY, POLİFENOL, KATEŞİN, HPLC

Kaynakça

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