Determination of Antibacterial Activity of Essential Oils from Aromatic Plants Against Pathogenic and Antibiotic Resistant Microorganisms in Animals and Animal Products

Yıl 2024, Cilt: 21 Sayı: 2, 257 - 263, 31.12.2024

Çiğdem Yamaner

https://doi.org/10.25308/aduziraat.1578360

Öz

Dairy calf mastitis is a relatively common infectious condition that mostly results from Staphylococcus aureus infections. It causes significant economic losses for dairy farmers all over the world. Due to the misuse of drugs, drug-resistant pathogens have started to emerge. Since these pathogens do not respond to commonly used treatments, the economic loss is increasing every year. In this study, the antimicrobial activity of three different plants (Origanum onites, Teucrium polium and Vitex agnus-castus) collected from Aydın and its surroundings on six different antibiotic-resistant microorganisms some of which are the causative agents of mastitis, was determined using agar disc diffusion methods. The essential oil of O. onites had 32 mm inhibition zone against MRSA, while the antibiotic used as standard had no effect. The essential oil of T. polium showed the smallest inhibition zone (3 mm) against MRSA and the biggest inhibition zones (20.5 mm) against Pseudomonas aeruginosa. Extracts of V. agnus-castus formed zones of inhibition against Bacillus cereus CCM99 and Staphylococcus aureus ATCC 6538 of 23.5 and 36 mm, respectively. In addition, comparing the data of this study with literature data, it is suggested that β-farnesene in T. polium, carvacrol and o-cymene in O. onites and 1,8-cineole in V. agnus-cactus L. may be more effective in antibacterial activity than other essential oil constituents. This study has shown that the essential oils of these plants could be important components of drugs that can be employed against multidrug-resistant pathogens that cause disease in humans and animals, many of which are foodborne.

Anahtar Kelimeler

Essential oil, mastitis, Origanum onites, Teucrium polium, Vitex agnus-castus.

Aromatik Bitkilerden Elde Edilen Uçucu Yağların Hayvanlarda ve Hayvansal Ürünlerde Patojen ve Antibiyotiklere Dirençli Olan Mikroorganizmalara Karşı Antibakteriyel Aktivitesinin Belirlenmesi

Öz

Sığırlarda mastitis, çoğunlukla Staphylococcus aureus enfeksiyonlarından kaynaklanan nispeten yaygın bulaşıcı bir hastalıktır. Tüm dünyada süt üreticileri için önemli ekonomik kayıplara neden olmaktadır. İlaçların yanlış kullanımı nedeniyle ilaca dirençli patojenler ortaya çıkmaya başlamıştır. Bu patojenler yaygın olarak kullanılan tedavilere yanıt vermediği için ekonomik kayıp her geçen yıl artmaktadır. Bu çalışmada, Aydın ve çevresinden toplanan üç farklı bitkinin (Origanum onites, Teucrium polium ve Vitex agnus-castus L.), bazıları mastitis etkeni ve antibiyotik dirençli olan altı farklı mikroorganizma üzerindeki antimikrobiyal aktivitesi agar disk difüzyon yöntemleri kullanılarak belirlenmiştir. O. onites uçucu yağı MRSA'ya karşı 32 mm inhibisyon zonu oluştururken, standart olarak kullanılan antibiyotiğin hiçbir etkisi olmamıştır. T. polium uçucu yağı MRSA'ya karşı en küçük (3 mm) ve Pseudomonas aeruginosa'ya karşı en büyük (20,5 mm) inhibisyon zonunu göstermiştir. V. agnus-castus ekstraktları Bacillus cereus CCM99 ve Staphylococcus aureus ATCC 6538'e karşı sırasıyla 23,5 ve 36 mm'lik inhibisyon bölgeleri oluşturmuştur. Ayrıca, bu çalışmanın verileri literatür verileri ile karşılaştırıldığında, T. polium'daki β-farnesen, O. onites'deki karvakrol ile o-simen ve V. agnus-cactus L.'deki 1,8-sineolün antibakteriyel aktivitede diğer uçucu yağ bileşenlerine göre daha etkili olabileceği düşünülmektedir. Bu çalışma, bu bitkilerin uçucu yağlarının, insanlarda ve hayvanlarda hastalığa neden olan ve çoğu gıda kaynaklı olan çoklu ilaç direncine sahip patojenlere karşı kullanılabilecek ilaçların önemli bileşenleri olabileceğini göstermiştir.

Anahtar Kelimeler

Esansiyel yağ, mastitis, Origanum onites, Teucrium polium

Kaynakça

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