Yeni İzole Edilmiş Trametes versicolor’un Tekrarlı-Kesikli Fermentasyon Sürecinde Eksopolisakkarit Üretme Potansiyelinin Araştırılması

Öz

Beyaz çürükçül funguslar ekzopolisakkarit (EPS) üretebilir ve bu EPS'lerin çeşitli uygulamalarda kullanılma potansiyeli vardır. Beyaz çürükçül fungus olan Trametes versicolor da yüksek miktarda EPS üretebilir. EPS üretimi fermentasyon metodu, üretim koşulları, ortamdaki besin kaynakları (özellikle glukoz konsantrasyonu) ve ayrıca kullanılan suşa bağlı olarak değişir. Bu nedenle, bu çalışmada öncelikle Hatay/Türkiye'den toplanan T. versicolor suşunun EPS üretim yeteneği tekrarlı-kesikli fermentasyon (TKF) sürecinde araştırılmıştır. T. versicolor, TKF koşulunda inkübe edildi. Tekrarli-kesikli süreç sırasında farklı ortamlarda EPS üretimi araştırıldıktan sonra, besiyeri alıkonma süresinin EPS üretimi üzerindeki etkisi belirlendi. Daha sonra da çalkalama, sıcaklık, pH, kullanılan pelet miktarı ve glukoz konsantrasyonun EPS üretimi üzerindeki etkileri belirlendi. Çalışmanın sonuçları, hem üretim koşullarının hem de glukoz konsantrasyonun bu suşun TKF sürecinde EPS üretimini etkilediğini göstermiştir.

Anahtar Kelimeler

• Beyaz çürükçül fungus; Ekzopolisakkarit
• Tekrarlı-kesikli fermentasyon
• Trametes versicolor

Investigation the Exopolysaccharide Production Potential of Newly Isolated Trametes versicolor during Repeated-Batch Fermentation

Öz

White rot fungi can produce exopolysaccharides (EPS) and these EPSs have the potential to be used in various applications. Trametes versicolor, a white rot fungus, can also produce high amount of EPS. EPS production varies depending on fermentation method, production conditions, nutrient sources (especially glucose concentration) in the medium and also the strain used. Therefore, in this study, EPS production ability of T. versicolor strain collected from Hatay/Turkey was firstly investigated during the repeated-batch fermentation (RBF) process. T. versicolor was incubated under RBF condition. After investigating the EPS production in different media during repeated-batch process, the effect of medium retention time on EPS production was determined. Then; the effects of agitation, temperature, pH, amount of pellets used and amount of glucose on EPS production were determined. The results of the study showed that both production conditions and glucose concentration affect the EPS production of this strain during the RBF process.

Anahtar Kelimeler

• Exopolysaccharide
• Repeated-batch fermentation
• Trametes versicolor
• White rot fungus

Kaynakça

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