Investigation of Biological Activities of Some Microalgae Extract Isolated from Kabakli Pond (Diyarbakır) Turkey

Yıl 2024, Cilt: 14 Sayı: 2, 59 - 77, 31.12.2024

Feysel Çakmak , A. İsmail Özkan , Nesrin Haşimi , Özlem Demirci , Melike Ciniviz , Lokman Varışlı , Ersin Kılınç , Veysel Tolan

https://doi.org/10.37094/adyujsci.1566859

Öz

The antimicrobial, antioxidant and mutagenic activities of methanolic extracts of microalgae species isolated from Kabakli Pond (37° 55' 23N, 40°17' 40E, Diyarbakır) and identified as Chlorella vulgaris Beyerinck and Chroococcus limneticus Lemmermann were investigated. The highest antioxidant activity was observed in the ABTS•+ assay of C. vulgaris (36.63%) and C. limneticus (28.48%) at a concentration of 100 µg/ml concentration. The DPPH and CUPRAC assays showed weak activity. The antioxidant activity did not appear to be significant for either species when compared with the positive controls. C. vulgaris showed high antimicrobial activity with inhibition zone and MIC value against S. aureus. There seemed to be no mutagenic activities; however, C. limneticus showed an effect on the colony structure of S. typhimurium strain TA98. C. vulgaris has a good antimicrobial potential, whereas C. limneticus has relatively weak potential. C. limneticus a relatively has a higher antioxidant activity compared to C. vulgaris, but the activities of both extracts are weak compared to the controls. None of the tested extracts showed mutagenic activity against S. typhimurium TA98 at any of the concentrations. These organisms via different solvents or extraction systems since they may affect various biological systems.

Anahtar Kelimeler

antioxidant, mutageicity, biological activity, Microalgae

Etik Beyan

We intend to publish an article entitled “Investigation of Biological Activities of Some Microalgae Extracts Isolated from Kabakli Pond (Diyarbakır) Turkey” by Feysel Çakmak, Ahmet İsmail Özkan, Nesrin Haşimi, Özlem Demirci, Melike Ciniviz, Lokman Varişli, Ersin KILINÇ and Veysel Tolan, in your esteemed journal as a research article. On behalf of all the contributors I will act and guarantor and will correspond with the journal from this point onward. We confirm that this work is original and has not been published previously, in identical or slightly modified form, nor has it been submitted to another journal for publication at the same time, proper citations to the previously reported works have been given, and, in case data /figures are quoted verbatim from some other publication, the required permission to do so has been obtained. All co- authors have been informed about the contents of the manuscript and its submission.

Destekleyen Kurum

Dicle üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Proje Numarası

DÜBAP Fen.17.020

Teşekkür

This Project was supported by the Dicle University, Coordinatorship of Scientific Research Projects (DUBAP) Fen.17.020.

Kabaklı Göleti'nden (Diyarbakır) İzole Edilen Bazı Mikroalg Ekstraktlarının Biyolojik Aktivitelerinin Araştırılması

Öz

Kabaklı Göleti'nden (37° 55' 23K, 40°17' 40D, Diyarbakır) izole edilen ve Chlorella vulgaris Beyerinck ve Chroococcus limneticus Lemmermann olarak tanımlanan mikroalg türlerinin metanol ekstraktlarının antimikrobiyal, antioksidan ve mutajenik aktiviteleri araştırıldı. En yüksek antioksidan aktivite, 100 µg/ml konsantrasyonunda C. vulgaris (%36,63) ve C. limneticus (%28,48) için ABTS•+ analizinde gözlendi. DPPH ve CUPRAC analizleri zayıf aktivite gösterdi. Antioksidan aktivite, pozitif kontrollerle karşılaştırıldığında her iki tür için de önemli görülmedi. C. vulgaris metanol ekstraktlarının S. aureus'a karşı inhibisyon zonu ve MİK değeri ile yüksek antimikrobiyal aktivite gösterdi. C. limneticus metanol ekstraktlarının S. typhimurium TA98 üzerinde herhangi bir mutajenik aktivitesi gözlenmedi. C. vulgaris metanol ekstraktı iyi bir antimikrobiyal potansiyele sahipken, C. limneticus ektraktı nispeten zayıf bir potansiyele sahiptir. C. limneticus metanol ekstraktı, C. vulgaris metanol ekstraktına kıyasla nispeten daha yüksek bir antioksidan aktiviteye sahip ancak her iki özütün aktiviteleri kontrollerle karşılaştırıldığında zayıf olarak gözlendi. Test edilen özütlerin hiçbiri, herhangi bir konsantrasyonda S. typhimurium TA98'e karşı mutajenik aktivite göstermedi. Bu organizmalar farklı çözücüler veya ekstraksiyon sistemleri aracılığıyla çeşitli biyolojik sistemleri etkileyebilirler.

Anahtar Kelimeler

Mikroalg, Mutajenite, Biyolojik aktivite, Antioksidan, Antimikrobiyal

Proje Numarası

DÜBAP Fen.17.020

Kaynakça

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