LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION

Filiz Boran; Özfer Yeşilada

doi:10.54365/adyumbd.1107682

TR EN

YENİ İZOLE EDİLMİŞ GANODERMA LUCIDUM İLE KATI HAL FERMANTASYONU KOŞULLARINDA LAKKAZ ÜRETİMİ VE BOYA RENK GİDERİMİNDE KULLANILMASI

Öz

Yeni izole edilmiş fungus Ganoderma lucidum' un lakkaz üretim kapasitesi, katı substratlar üzerinde katı ortam fermantasyonu sırasında incelenmiştir. Buğday kepeğinin en etkili katı substrat olduğu tespit edildiğinden, buğday kepeği ortamında çeşitli kültür parametrelerinin ve bazı indükleyicilerin lakkaz üretimine etkisi araştırılmıştır., pH 5.0, 30 °C ve %75 nem içeriği ile buğday kepeği en etkili ortamdı ve 10 mM bakır ilavesi en yüksek lakkaz miktarını destekledi. Cam tava fermentörde 2973±220 U/L lakkaz aktivitesi elde edildi. Ham lakkaz 70 °C'de en yüksek aktiviteyi vermiştir. 60°C'de 6 saat stabildi ve 70°C'de 2 saat boyunca %37 aktiviteyi korudu. Ham lakkaz, 20 dakika içinde Remazol Parlak Mavi R' nin %61 rengini giderdi. Sonuçlar, bu suşun yüksek miktarda lakkaz üretebileceğini ve bakır gibi uygun indükleyicilerin lakkaz üretimini indükleyebileceğini gösterdi. Elde edilen ham lakkaz, tekstil boyası renk giderme uygulamaları için kullanılabilir.

Anahtar Kelimeler

Ganoderma lucidum , Lakkaz , Remazol Parlak Mavi R , Katı ortam fermentasyonu , Buğday kepeği

LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION

Abstract

Laccase production capacity of newly isolated fungus Ganoderma lucidum was studied during solid state fermentation on solid substrates. Because wheat bran was detected as the most effective solid substrate, effect of various culture parameters and some inducers on laccase production in wheat bran media was investigated. Wheat bran with moisture content of 75%, pH 5.0 and 30 °C was the most effective medium and 10 mM copper addition supported the highest amount of laccase. In glass tray fermenter 2973±220 U/L laccase activity was obtained. Crude laccase gave the highest activity at 70 °C. It was stable at 60°C for 6 hours and retained 37% activity at 70 °C for 2 hours. It decolorized 61% of Remazol Brilliant Blue R within 20 min. Results showed that this strain could produce high amount of laccase and suitable inducer such as copper could induce its laccase production. The crude laccase obtained could be used for textile dye decolorization applications.

Keywords

Ganoderma lucidum , Laccase , Remazol Brilliant Blue R , Solid state fermentation , Wheat bran

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Mühendislik

Bölüm

Araştırma Makalesi

Yazarlar

Filiz Boran ^*
0000-0002-8801-7987
Türkiye

Özfer Yeşilada
0000-0003-0038-6575
Türkiye

Yayımlanma Tarihi

31 Ağustos 2022

Gönderilme Tarihi

22 Nisan 2022

Kabul Tarihi

20 Mayıs 2022

Yayımlandığı Sayı

Yıl 1970 Cilt: 9 Sayı: 17

DOI

https://doi.org/10.54365/adyumbd.1107682

IZ

https://izlik.org/JA88CP72YF

APA

Boran, F., & Yeşilada, Ö. (2022). LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION. Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi, 9(17), 458-470. https://doi.org/10.54365/adyumbd.1107682

AMA

1.Boran F, Yeşilada Ö. LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION. Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi. 2022;9(17):458-470. doi:10.54365/adyumbd.1107682

Chicago

Boran, Filiz, ve Özfer Yeşilada. 2022. “LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION”. Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi 9 (17): 458-70. https://doi.org/10.54365/adyumbd.1107682.

EndNote

Boran F, Yeşilada Ö (01 Ağustos 2022) LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION. Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi 9 17 458–470.

IEEE

[1]F. Boran ve Ö. Yeşilada, “LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION”, Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi, c. 9, sy 17, ss. 458–470, Ağu. 2022, doi: 10.54365/adyumbd.1107682.

ISNAD

Boran, Filiz - Yeşilada, Özfer. “LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION”. Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi 9/17 (01 Ağustos 2022): 458-470. https://doi.org/10.54365/adyumbd.1107682.

JAMA

1.Boran F, Yeşilada Ö. LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION. Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi. 2022;9:458–470.

MLA

Boran, Filiz, ve Özfer Yeşilada. “LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION”. Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi, c. 9, sy 17, Ağustos 2022, ss. 458-70, doi:10.54365/adyumbd.1107682.

Vancouver

1.Filiz Boran, Özfer Yeşilada. LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION. Adıyaman Üniversitesi Mühendislik Bilimleri Dergisi. 01 Ağustos 2022;9(17):458-70. doi:10.54365/adyumbd.1107682

Cited By

Laccase Production of Newly Isolated Trametes versicolor under Batch, Repeated-Batch, and Solid-State Fermentation Processes

Commagene Journal of Biology

https://doi.org/10.31594/commagene.1197055

YENİ İZOLE EDİLMİŞ GANODERMA LUCIDUM İLE KATI HAL FERMANTASYONU KOŞULLARINDA LAKKAZ ÜRETİMİ VE BOYA RENK GİDERİMİNDE KULLANILMASI

Öz

Anahtar Kelimeler

LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Laccase Production of Newly Isolated Trametes versicolor under Batch, Repeated-Batch, and Solid-State Fermentation Processes