Kuersetinin İnsan Meme Kanseri Hücre Hattı Üzerine Etkisinin İncelenmesi

Yıl 2022, , 261 - 269, 25.12.2022

Münevver Baran , Özge Göktepe , Gözde Özge Önder , Zeynep Burcin Gonen , Arzu Yay

https://doi.org/10.46332/aemj.1005558

Öz

Amaç: Meme kanseri, farklı epigenetik değişiklikler ve genetik mutasyonlar nedeniyle ortaya çıkan yaygın bir kanserdir. Doğal olarak oluşan Kuersetin, antioksidan, anti-inflamatuar ve anti-kanser özellikler sergileyen ve çok sayıda hücre içi hedefi yöneten bir flavonoid’dir. Bu çalışma da, MCF-7 meme kanseri hücre hattında Kuersetinin apopitoz /otofaji süreçleri üzerine olası etkisi-nin incelenmesi amaçlandı.

Araçlar ve Yöntem: Kuersetinin IC50 değerini 3-4.5-dimetil-tiyazolil–2.5-difeniletrazolyum bromid (MTT) analiz ile belirlemek için MCF-7 meme kanseri hücre hattına farklı konsantrasyonlar da ve sürelerde (24, 48 ve 72. saatte) kuersetin uygulandı ve gruplar oluşturuldu. Kontrol grubu; hiç bir uygulama yapılmayan grup, Kuersetin 1; kuersetinin IC50 dozunun uygulandığı grup, Kuersetin 2; kuersetin’in IC50 dozunun yarısının uygulandığı grup, D 1; kuersetin 1 grubunda kuersetin hazırlanmasında kullanı-lan DMSO oranının uygulandığı grup ve D 2; kuersetin 2 grubun da kuersetin hazırlanmasında kullanılan DMSO oranının uygu-landığı grup. Kuersetinin apopitotik etkisini değerlendirmek için TUNEL boyama yöntemi kullanılırken, otofajik etkisini ortaya çıkarmak için LC3/2 ve Beklin-1 immünofloresan boyama yöntemi uygulandı.

Bulgular: Kuersetinin hücreler üzerine etkisine bakıldığında, hücre canlılığının doz ve zamana bağlı olarak inhibe olduğu görüldü. Bunun yanında otofaji belirteçleri olan Beklin-1 ve LC3/2 immunre aktivitesinin de arttığı gözlendi.

Sonuç: Sonuç olarak, kuersetinin apopitoz ve otofaji yolakları ile MCF-7 meme kanseri hücrelerini ölüme götürdüğü gözlendi.

Anahtar Kelimeler

Apoptoz, Kuersetin, MCF-7 hücre hattı, Otofaji

Investigation of the Effect of Quercetin on Human Breast Cancer Cell Line

Öz

Purpose: Breast cancer is a common cancer that occurs due to different epigenetic changes and genetic mutations. Naturally occurring Quercetin is a flavonoid that exhibits antioxidant, anti-inflammatory and anti-cancer properties and manages multiple intracellular targets. In this study, it was aimed to investigate the possible effect of Quercetin on apoptosis/autophagy processes in MCF-7 breast cancer cell line.

Materials and Methods: Quercetin was applied to MCF-7 breast cancer cell line at different concentrations and times (24, 48 and 72 hours) to determine the IC50 value of quercetin by 3-4.5-dimetil-tiyazolil–2.5-difeniletrazolyum bromid (MTT) analysis, and groups were formed. Control group; no treatment group, Quercetin 1; IC50 dose of quercetin administered group, Quercetin 2; group in which half the IC50 dose of quercetin was administered, D 1; In the quercetin 1 group, the DMSO ratio used in the preparation of the quercetin was applied, and D 2; In the quercetin 2 group, the DMSO ratio used in the preparation of the quer-cetin was applied, While TUNEL staining method was used to evaluate the apoptotic effect of quercetin, LC3/2 and Beclin-1 immunofluorescence staining method was applied to reveal its autophagic effect.

Results: When the effect of quercetin on cells was examined, it was observed that cell viability was inhibited depending on dose and time. In addition, it was observed that the autophagy markers Beclin-1and LC3/2 immunoreactivity increased, too.

Conclusion: In conclusion, it was observed that quercetin led to death of MCF-7 breast cancer cells through apoptosis and autophagy pathways.

Anahtar Kelimeler

Apoptosis, Autophagy, MCF-7 cell line, Quercetin

Kaynakça

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