COVID-19 Hastalarında Antioksidanların ve Oksidatif Hasarın Durumu

Yıl 2023, , 114 - 123, 16.04.2023

İrfan Binici , Hamit Hakan Alp , Zübeyir Huyut , Esra Gürbüz , Hülya Günbatar , Şükrü Akmeşe , Mustafa Kasım Karahocagil , Halil İbrahim Akbay

https://doi.org/10.46332/aemj.1152479

Cited By: 2

Öz

Amaç: COVID-19, son zamanlarda bir pandemiye neden olan ve insan sağlığını önemli ölçüde etkileyen bir viral hastalıktır. Bu çalışmada COVID-19'da süperoksit dismutaz, glutatyon peroksidaz, glutatyon, toplam tiyol, doğal tiyol, disülfid, oksidatif DNA hasarı ve malondialdehit düzeyleri araştırıldı.

Araçlar ve Yöntem: Bu çalışmaya revers transkriptaz-polimeraz zincir reaksiyonu ile COVID-19 tanısı konan 35 hasta ve 35 sağlıklı gönüllü dahil edildi. Enzim bağlantılı immünosorbent testi ile serum glutatyon, glutatyon peroksidaz, süperoksit dismutaz, doğal tiyol, toplam tiyol ve disülfid seviyeleri ve yüksek basınçlı-sıvı kromatografisi ile malondialdehit ve 8-hidroksi-2-deoksiguanozin/10⁶ deoksiguanozin seviyeleri ölçüldü.

Bulgular: COVID-19 hasta grubunda serum süperoksit dismutaz, glutatyon peroksidaz, malondialdehit, 8-hydroxy-2-deoksiguanozin/10⁶, disülfid düzeyleri sağlıklı kontrol grubuna göre daha yüksek iken, glutathione, toplam tiyol, doğal tiyol düzeyleri daha düşüktü. Ayrıca 8-hydroxy-2-deoxyguanosine/10⁶ deoxyguanosine ile glutatyon, doğal tiyol ve toplam tiyol arasında negatif, disülfid ile pozitif korelasyon vardı.

Sonuç: Bu çalışma, COVID-19 hastalarında serum süperoksit dismutaz, glutatyon peroksidaz, glutatyon, malondialdehit, 8-hydroxy-2-deoxyguanosine/10⁶ deoxyguanosine ve disülfid düzeylerinin arttığını ve glutatyon, toplam tiyol ve doğal tiyol düzeylerinin azaldığını ortaya koydu. Bu sonuçlar, COVID-19 hastalarında, antioksidan belirteç düzeylerinde azalma ve oksidatif stres belirteçlerinde artış olduğunu ortaya koydu.

Anahtar Kelimeler

GPx, SOD, oksidatif stress, tiyol/disülfit

Destekleyen Kurum

Yok

Proje Numarası

YOK

Teşekkür

Van Yüzüncü Yıl Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı'na katkılarından dolayı teşekkür ederiz.

The Status of Antioxidants and Oxidative Damage in Patients with COVID-19

Öz

Purpose: COVID-19 is a viral disease that has recently caused a pandemic and significantly affects human health. In this study, superoxide dismutase, glutathione peroxidase, glutathione, total thiol, natural thiol, disulfide, oxidative DNA damage and malondialdehyde levels in COVID-19 were investigated.

Materials and Methods: Thirty-five patients and 35 healthy volunteers were included in this study. The diagnosis of COVID-19 was made by reverse transcriptase-polymerase chain reaction. Serum glutathione, glutathione peroxidase, superoxide dismutase, natural thiol, total thiol and disulphide levels by enzyme-linked immunosorbent assay and malondialdehyde and 8-hydroxy-2-deoxyguanosine/10⁶ deoxyguanosine levels by high-pressure liquid chromatography measured.

Results: While serum superoxide dismutase, glutathione peroxidase, malondialdehyde, 8-hydroxy-2-deoxyguanosine/10⁶ deoxyguanosine, disulfide levels were higher in the COVID-19 patient group than in the healthy control group, glutathione, total thiol, natural thiol levels were lower. In addition, there was a negative correlation between 8-hydroxy-2-deoxyguanosine/10⁶ deoxyguanosine and glutathione, natural thiol and total thiol, and a positive correlation with disulfide.

Conclusion: This study revealed that serum superoxide dismutase, glutathione peroxidase, malondialdehyde, 8-hydroxy-2-deoxyguanosine/10⁶ deoxyguanosine, and disulfide levels increased and glutathione, thiol and natural thiol levels decreased in COVID-19 patients. These results revealed that there was a decrease in antioxidant marker levels and an increase in oxidative stress markers in COVID-19 patients.

Anahtar Kelimeler

GPx, SOD, oxidative stress, thiol/disulphide

Proje Numarası

YOK

Kaynakça

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