Investigation of the Efficacy of Topical and Subconjunctival Bevacizumab in Experimental Corneal Neovascularization in Rabbits

Yıl 2019, Cilt: 3 Sayı: 2, 54 - 58, 26.08.2019

Ümit Doğan , Serdal Çelebi Aysel Kükner

Öz

ABSTRACT

Purpose: To compare the
efficacy of topical and subconjunctival bevacizumab with topical dexamethasone
in the treatment of corneal neovascularization (NV) secondary to chemical burns in
experimental rabbit models.

Materials and Methods: A total of 24 rabbits were used in the study.
Animals were divided into four groups as Control (received topical 0.09% NaCl) (Group 1), topical
dexamethasone (Group 2), subconjunctival bevacizumab (Grup3) and topical
bevacizumab (Group 4). Chemical burns were created with silver nitrate-potassium
nitrate in cornea of the subjects. During the next 10 days, each group was
given the drug (0.09 % NaCl, dexamethasone or bevacizumab). On the 10th day, the
subjects were sacrificed and macroscopic and histopathological evaluation of
corneal neovascularization was performed.

Results: When the number of vessels in the histopathological examination was
evaluated; In Group 2 (5.03 ± 0.42), Group 3 (3.36 ± 0.15) and Group 4 (3.46 ± 0.24), corneal
neovascularization was significantly less than Group 1 (6.76 ± 1.03) (p
<0.05). When the groups except the control group were compared; the
number of vessels was significantly lower in Group 3 and Group 4 than Group 2
(p <0.05).

Conclusion: In this study, both topical and subconjunctival bevacizumab were found to
be more effective than topical dexamethasone in the treatment of corneal NV
secondary to chemical burn.  

Anahtar Kelimeler

Bevacizumab, corneal neovascularization, dexamethasone

Tavşanlarda Deneysel Kornea Neovaskülarizasyonunda Topikal ve Subkonjonktival Bevacizumabın Etkinliğinin Araştırılması

Öz

ÖZ

Amaç: Deneysel tavşan modellerinde oluşturulan kimyasal yanığa sekonder korneal neovaskülarizasyon (NV) tedavisinde topikal ve subkonjonktival bevacizumabın etkinliğini topikal deksametazonla karşılaştırmak.

Araçlar ve Yöntem: Çalışmada toplam 24 adet tavşan kullanıldı. Hayvanlar kontrol (topikal %0.09 NaCl) (Grup 1), topikal deksametazon (Grup 2), subkonjonktival bevacizumab (Grup3) ve topikal bevacizumab (Grup 4) olmak üzere dört gruba ayrıldı. Deneklerin kornealarında gümüş nitratpotasyum nitrat ile kimyasal yanık oluşturuldu. Sonraki 10 gün boyunca her gruba ilgili ilaç uygulandı. Onuncu günde denekler sakrifiye edilip makroskopik ve histopatolojik olarak korneal neovaskülarizasyon değerlendirildi.

Bulgular: Histopatolojik incelemede grupların damar sayıları değerlendirildiğinde; Grup 2 (5.03±0.42), Grup 3 (3.36±0.15) ve Grup 4 (3.46±0.24)’te korneal neovaskülarizasyonda Grup 1 (6.76±1.03)’e göre anlamlı düzeyde az olduğu gözlendi (p<0.05). Kontrol grubu dışındaki gruplar kendi içinde karşılaştırıldığında; damar sayısının Grup 3 ve Grup 4’te Grup 2’ye göre belirgin düzeyde az olduğu gözlendi (p<0.05).

Sonuç: Bu çalışmada kimyasal yanığa sekonder korneal NV tedavisinde hem topikal hem de subkonjonktival bevacizumabın, topikal deksametazondan daha etkili olduğunu gösterilmiştir.

Anahtar Kelimeler

Bevacizumab, Deksametazon, Korneal neovaskülarizasyon

Kaynakça

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