Rize ve Çevresinde Yetişen Yaban Meyvelerinden Elde Edilen Özütlerin Anti-Proliferatif Etkilerinin Araştırılması

Yıl 2019, Cilt: 3 Sayı: 3, 104 - 111, 03.12.2019

Saliha Ekşi , Sena Şahin Aktura Nebahat Ejder Kazım Şahin

Öz

ÖZ

Amaç: Doğu Karadeniz bölgesi, farklı tıbbi ve aromatik bitkileri barındıran
zengin bir floraya sahiptir. Rize ve çevresinde başta Arbutus unedo
(Kocayemiş), Aronia melanocarpa (Rus yaban mersini), Fragaria vesca
L. (Yabani çilek), Frangula alnus (Barut ağacı), Ribes rubrum
(Frenk üzümü) ve Solanum nigrum (Köpek üzümü) olmak üzere çeşitli
meyveler doğal olarak yetişmektedir. Bu çalışmada, adı geçen meyvelerden elde
edilen metanol özütlerinin insan retina pigment epitel hücreleri (ARPE-19) ve
insan serviks adenokarsinoma hücreleri (HeLa) üzerindeki antiproliferatif
etkinliklerinin değerlendirilmesi amaçlandı.

Araçlar
ve Yöntem: Meyvelerin metanol özütleri elde edilerek, çeşitli
konsantrasyonlarda ARPE-19 ve HeLa hücre kültür ortamlarına eklendi. Pozitif ve
negatif kontrol olarak sırasıyla, Taksol ve DMSO kullanıldı. 48 saatlik
inkübasyon sonrası özütlerin hücreler üzerindeki sitotoksik etkileri MTT testi
ile değerlendirildi. İstatistiksel analizler bağımsız örneklem t-testi
kullanılarak gerçekleştirildi. p değeri 0.05’ten küçük olan sonuçlar anlamlı
olarak kabul edildi.

Bulgular: R.rubrum ve S.nigrum özütleri iki hücre hattında da doza
bağımlı sitotoksik aktivite göstermişler, buna ek olarak A.melanocarpa
ve F.vesca özütleri yüksek konsantrasyonlarda her iki hücre hattı
üzerinde sitotoksik etki göstermişlerdir. A.unedo ve F.alnus
metanol özütlerinin sadece HeLa üzerinde doza bağımlı olarak hücre canlılığını
inhibe ettiği tespit edilmiştir.

Sonuç: A.unedo, A.melanocarpa, F.vesca, F.alnus, R.rubrum
ve S.nigrum’un hem ARPE-19 hem de HeLa üzerindeki sitotoksik etkileri
ilk defa bu çalışmada araştırılmıştır. A.unedo ve F.alnus  bitki ekstrelerinin  antiproliferatif ve sekonder bileşenlerini
belirlemek için daha fazla çalışmaya ihtiyaç duyulmaktadır. 

Anahtar Kelimeler

Serviks kanseri, sitotoksisite, Arbutus unedo, Frangula alnus, metiltiazol difenil tetrazolyum

Destekleyen Kurum

Recep Tayyip Erdoğan Üniversitesi

Proje Numarası

BAP-2015.53001.106.01.04

Teşekkür

Bu çalışma Recep Tayyip Erdoğan Üniversitesi BAP-2015.53001.106.01.04 nolu proje ile desteklenmiştir. Çalışmada kullanılan HeLa hücre hattını bize temin eden Prof. Dr. Muradiye Acar’a ve ARPE-19 hücre hattını sağlayan Prof. Dr. Fikrettin Şahin’e teşekkür ederiz.

Investigation of Anti-Proliferative Activities of Extracts Derived From Wild Fruits That Grow in Rize Vicinity

Öz

ABSTRACT

Purpose: Eastern Black Sea region has a rich flora with
different medicinal and aromatic plants. In particular, Arbutus unedo (Strawberry tree), Aronia melanocarpa (Russian bilberry), Fragaria vesca L. (Wild strawberry), Frangula alnus (Alder dogwood), Ribes
rubrum (Red currant) and Solanum
nigrum (Nightshade), various fruits grow indigenous to Rize and its
vicinity. The aim of the study was to evaluate the anti-proliferative activity
of these fruits on human retinal pigment epithelial cells (ARPE-19) and human
cervix adenocarcinoma cells (HeLa). 

Materials and Methods: Methanol extracts of the
fruits were obtained and added to ARPE-19 and HeLa culture medias at various
concentrations. Taxol and DMSO were used as positive and negative controles,
respectively. After 48 hours of cell incubation, the cytotoxic effect of the
extracts was assessed by MTT. Cell viability values ​​were calculated for each
group. The statistical analysis of the data was performed using unpaired
t-test. p values ​​less than 0.05 were considered significant.

Results: The extracts of R.rubrum and S.nigrum  showed dose dependent cytotoxic activity
against both cell lines. In additions, A.melanocarpa
and F.vesca  extracts exhibited cytotoxic effect on
both cell lines at higher concentrations. Methanol extracts obtained from A.unedo and F.alnus  were found to decrease cell viability in a
dose-dependent manner only in HeLa cells.

Conclusion: The cytotoxic effects of A.unedo, A.melanocarpa, F.vesca,
F.alnus, R.rubrum  ande S.nigrum’un  on both ARPE-19 and HeLa
were firstly investigated in this study.
To underline the antiproliferative effects of. A.unedo  and F.alnus   extracts as well as their seconder  components further studies are needed. 

Anahtar Kelimeler

Cervical cancer, cytotoxicity, Arbutus unedo, Frangula alnus, methylthiazole diphenyl tetrazolium

Proje Numarası

BAP-2015.53001.106.01.04

Kaynakça

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