SDS-PAG Elektroforezinin Et ve Sakatat Analizinde Kullanılması

Özgür Kaynar; İsmail Erim Köseoğlu

SDS-PAG Elektroforezinin Et ve Sakatat Analizinde Kullanılması

Öz

Sodyum Dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE), bir protein karışımının bileşenleri ayrı ayrı çözmek için en yaygın kullanılan analitik yöntemdir. Ayrıca bir proteinin saflığını elektroforetik bir yöntemle değerlendirmek neredeyse zorunlu hale gelmiştir. SDS-PAGE, proteinlerin moleküler boyuttaki farklılıkları ile jel matrisi boyunca %1' den daha az farklılık gösteren elektroforetik hareketliliklerini aynı anda kullanarak proteinleri birbirinden ayırır. Teknik aynı zamanda proteinlerin moleküler ağırlıklarını tahmin etme de güçlü bir araçtır. Protein analizinde vazgeçilmez bir araç olarak SDS-PAGE'in başarısı, elektroforetik hareketliliğin bir proteinin moleküler kütlesi ile korelasyonuna izin vermektedir. Ve günümüzde başarılı bir SDS-PAGE analizi sadece ve sadece dikkatli numune hazırlama ve yüklemeden önce işlemlere bağlıdır. Bu makalede en basit SDS-PAGE analizinin farklı et ve sakatat örneklerinin ayrımında kullanılabileceği anlatılmaktadır.

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Analitik Biyokimya , Gıda Mühendisliği

Bölüm

Araştırma Makalesi

Yazarlar

Özgür Kaynar ^*
0000-0002-2875-423X
Türkiye

İsmail Erim Köseoğlu
0000-0001-7924-8209
Türkiye

Yayımlanma Tarihi

30 Eylül 2022

Gönderilme Tarihi

21 Temmuz 2022

Kabul Tarihi

13 Eylül 2022

Yayımlandığı Sayı

Yıl 2022 Sayı: 4

IZ

https://izlik.org/JA98BT87LY

Kaynak Göster

RIS / Bibtex

APA

Kaynar, Ö., & Köseoğlu, İ. E. (2022). SDS-PAG Elektroforezinin Et ve Sakatat Analizinde Kullanılması. Akademik Et ve Süt Kurumu Dergisi, 4, 25-34. https://izlik.org/JA98BT87LY