İNCE BAĞIRSAK KARBONİK ANHİDRAZ AKTİVİTESİ ÜZERİNE ORNİDAZOL VE METRONİDAZOL İLAÇLARININİN VİTROETKİSİ

Yıl 2017, Cilt: 1 Sayı: 1, 1 - 13, 01.01.2017

Fatma Ergün Dilek Dülger Nazan Demir

Öz

Bu araştırmada sığır ince bağırsağından total olaraksaflaştırılan karbonik anhidraz EC 4.2.1.1. enzimi aktivitesiüzerine bazı ilaçlarınBağırsakafinitekromatografisi ile saflaştırıldı. Saf olarak elde edilmişolan karbonik anhidraz enziminin aktivitesi üzerine ornidazolve metronidazol etken maddesine sahip ilaçların etkileriaraştırıldı. Aktivite ölçümlerinde karbonik anhidrazın CO2hidrataz aktivitesinden yararlanıldı. 3 farklı konsantrasyondave 6 farklı hacimde yapılan ölçümlerin sonuçları Aktivite-[İlaçhacmi] şeklinde grafik halinde verildi. Elde edilen bulgulardan,ornidazol ve metronidazol ilaçlarının bağırsak CA enzimaktivitesini aktive ettiği belirlendi

Anahtar Kelimeler

İnce bağırsak, Karbonik anhidraz, Ornidazol, Metronidazol

Kaynakça

• Altıntop, M.D.,Ozdemir, A., Kucukoglu, K., Turan-Zitouni, G., Nadaroglu, H., Kaplancıklı, Z.A.(2015). Synthesisand Evaluation of New ThiadiazoleDerivatives as PotentialInhibitors of Human CarbonicAnhydraseIsozymes (hCA-I andhCA-II), Journal of EnzymeInhibitionandMedicinalChemistry, 30(1), 32-37. http://dx.doi.org/10.3109/14756366.2013.873038
• Altıntop, M.D., Sever, B., Çiftçi, G.A., Kucukoglu, K., Ozdemir, A., Soleimani, S.S., Nadaroglu, H., Kaplancıklı, Z.A.(2017a).SynthesisandEvaluation of New Benzodioxole-BasedDithiocarbamateDerivatives as PotentialAnticancerAgentsandhCA-I andhCA-II İnhibitors. EuropeanJournal of MedicinalChemistry, 125, 190-196.https://doi.org/10.1016/j.ejmech.2016.09.035
• Altıntop, M.D., Sever, B., Özdemir, A., Kucukoglu, K., Onem, H.,Nadaroglu, H.,Kaplancıklı, Z.A.(2017b).Potentialinhibitors of humancarbonicanhydraseisozymes I and II: Design, synthesisanddockingstudies of new1,3,4-thiadiazole derivatives, Bioorganic&MedicinalChemistry, 25(13), 3547-3554. doi:http://dx.doi.org/10.1016/j.bmc.2017.05.005.
• Anderson, A.K. (2005). AffectiveInfluences on theAttentional Dynamics SupportingAwareness. Journal of ExperimentalPsychology: General, 154, 258–281. doi:10.1037/0096- 3445.134.2.258
• Arslan, O., Nalbantoğlu, B., Demir, N., Özdemir, H., Küfrevioğlu, Ö.İ. (1996) A New MethodforthePurification at CarbonicAnhydraseIsozymesbyAffinityChromatography. Turk J MedSci 26: 163-166
• Bradford, M.M. (1976). A rapidandsensitivemethodforthequantitation of microgramquantities of protein utilizingtheprinciple of protein-dyebinding. Anal Biochem 72: 248-254. doi.org/10.1016/0003-2697(76)90527-3
• Britta, K.,Ralf, R. (1997). β-lactamantibioticsinhibitchloroplastdivision in a moss (Physcomitrellapatens) but not in tomato (Lycopersiconesculentum): Journal of PlantPhysiology, 150, 137- 140.https://doi.org/10.1016/S0176-1617(97)80193-9
• Demir, Y.,Nadaroğlu, H., Demir, N. 2004. Effects of omeprazole, famotidine, andranitidine on theenzymeactivities of carbonicanhydrasefrombovinestomach in vitroandraterythrocytes in vivo, BiologicalandPharmaceuticalBulletin, 27, 1730-1734. http://doi.org/10.1248/bpb.27.1730
• Demir, Y.,Nadaroğlu, H., Demir, N.(2006) Effect of Glimepiride on Paraoxonase Activity: PharmaceuticalBiology, 44 (5), 396–399. http://dx.doi.org/10.1080/13880200600751717
• Ekinci, D.,Beydemir, S., Alim, Z. 2007. Somedrugsinhibit in vitrohydrataseandesteraseactivities of humancarbonicanhydrase-I and II, PharmacologicalReports, 59, 580-587.
• Fleming, R.E.,Parkkila, S., Parkkila, A.K., Rajaniemi, H., Waheed, A., Sly, W.S. (1995) Carbonicanhydrase IV expression in ratandhumangastrointestinaltrackregional, cellularandsubcellularlocalization. J. Chin. İnvest. 96, 2907-2913.doi: 10.1172/JCI118362
• Hansson, H.P.J. (1967) HistokimyasalDemonstration of CarbonicAnhydrase Activity. Histochemie. 11, 112-128.
• Hewett-Emmett, D. (2000) Inthecarbonicanhtdrase- newhorizons. BirkhauserVerlag, Basel, 29-78.
• Kohn, J.,Wilchek, M. (1978) Colorimetricmethodformonitoringactivation of sepharosebycynogenbromide. Biochem. Biophys. Res. Commun. 7, 7-14. https://doi.org/10.1016/0006-291X(78)90255-3
• Löfmark, S.,Edlund, C. andNord, C.E. Metronidazole Is StilltheDrug of ChoiceforTreatment of AnaerobicInfectionsClinicalInfectiousDiseases 2010; 50:S16–23 doi:https://doi.org/10.1086/647939
• Maren TH (1967) Carbonic Anhydrase, Chemistry, Physiology and Inhibition. Physiological McIntosh, J.E.A. (1969) Carbonikanhydraseisoenzymes in theerythrocytesanddorsdateralprostate of rat. Biochem. J. 114, 463-467.
• Onat, T.,Emerk, K. (1997) Temel Biyokimya, Saray Medikal Yayıncılık, (2. Baskı) İzmir.
• Pocker, Y.,Sarkanen, S. (1978) Carbonicanhydrase: Structure, catalyticversalityandinhibition. Adv. Enzym. 49, 149-274.
• Rickli, E.E., Ghazanfar, S.A.S., Gibbons, B.H. and Edsall, J.T (1964) Carbonic Anhydrases from Human Erythrocytes. Journal of Biological Chemistry 239: 1065-1078
• Sly, S.W., Peigi, Y.H. (1995) Human carbonic anhydrase and carbonic andrase deficiencies. Annu. Rev. Biochem., 64, 375-401. https://doi.org/10.1146/annurev.bi.64.070195.002111
• Whitney, P.L.,Briggle, T.V (1982) Membrane-associatedcarbonicanhydrasepurifiedfrombovinelun g. J. Biol. Chem. 257, 12056-12059.
• Zhu, X.L.,Sly, W.S. (1990) Carbonicanhydrase IV fromhumanlung. Purification, characterizationandcomrarisonwithmembranecarbonicandydra sefromhumankidney. J. Biol. Chem. 265, 8795-8801.