The influence of tannins purified from Eastern Mediterranean Region plants (Pinus brutia Ten. and Quercus coccifera L.) on carbon mineralization: Antimicrobial and antimutagenic evaluation

Yıl 2023, , 60 - 69, 15.05.2023

Funda Ulusu , Cengiz Darıcı

https://doi.org/10.30616/ajb.1259084

Öz

Tannins, which are polyphenols with a wide variety of quality-quantity that control the carbon and nitrogen cycle in forest ecosystems, are very interesting because of their protein binding abilities and forming a complex structure with other compounds. In this study, the purified tannin content of Pinus brutia Ten. and Quercus coccifera L., the two dominant plant species of the Eastern Mediterranean region, and the effect of these tannins on C dynamics in a forest soil (O and A horizon) were evaluated. In addition, antimicrobial effects of tannin extracts on Bacillus subtilis, Staphylococcus aureus and Proteus mirabilis bacteria by disc diffusion method and antimutagenic effects on Allium cepa root tip cells were evaluated. Total phenol (TP) and condense tannins (CT) concentrations of P. brutia and Q. coccifera leaves ranged from 0.78–1.33 μg/100mg DW and 4.68–1.35 μg/100mg DW, respectively. With the addition of tannin extract to the soils, C mineralization (27th day) was significantly reduced compared to the control group. Both P. brutia tannin extract (PTE) and Q. coccifera tannin extract (QTE) exhibited antibacterial activity in the range of 8±0.2–35±1.1 mm zone diameter by inhibiting their microbial growth against test microorganisms. In addition, tannin treatments caused a dose-dependent mitotic index decrease in onion root tip cells and a serious inhibition by showing toxic effects on mitotic division stages. As a result, our data showed that C mineralization in soil is affected by different tannin sources and these tannin extracts have significant antimicrobial activity against pathogens and cytotoxic activity in A. cepa root tip cells.

Anahtar Kelimeler

Carbon mineralization, tannin, antimicrobial activity, antimutagenic activity

Destekleyen Kurum

Çukurova Üniversitesi

Proje Numarası

FEF2006YL59

Teşekkür

Supported by Cukurova University Research Projects Unit under Project no FEF2006YL59.

Doğu Akdeniz Bölgesi bitkilerinden (Pinus brutia Ten. ve Quercus coccifera L.) saflaştırılan tanenlerin karbon mineralizasyonu üzerindeki etkisi: Antimikrobiyal ve antimutajenik değerlendirme

Öz

Orman ekosistemlerinde karbon ve nitrojen döngüsünü kontrol eden çok çeşitli kalite-niceliğe sahip polifenoller olan tanenler, protein bağlama yetenekleri ve diğer bileşiklerle kompleks bir yapı oluşturmaları nedeniyle oldukça ilgi çekicidir. Bu çalışmada, Doğu Akdeniz bölgesinin iki baskın bitki türü olan Pinus brutia Ten. ve Quercus coccifera L.'nın saflaştırılmış tanen içerikleri ve bu tanenlerin bir orman toprağında (O ve A horizonu) C dinamiklerine etkisi değerlendirilmiştir. Ayrıca tanen ekstraktlarının disk difüzyon yöntemi ile Bacillus subtilis, Staphylococcus aureus ve Proteus mirabilis bakterileri üzerindeki antimikrobiyal etkileri ve Allium cepa kök ucu hücreleri üzerindeki antimutajenik etkileri değerlendirilmiştir. P. brutia ve Q. coccifera yapraklarının toplam fenol (TP) ve kondanse tanen (CT) konsantrasyonları sırasıyla 0.78–1.33 μg/100mg DW ve 4.68–1.35 μg/100mg DW arasında değişmiştir. Topraklara tanen ekstraktı ilavesi ile C mineralizasyonu (27. gün) kontrol grubuna göre önemli ölçüde azalmıştır. Hem P. brutia tanen ekstresi (PTE) hem de Q. coccifera tanen ekstresi (QTE), test edilen mikroorganizmalara karşı 8±0.2–35±1.1 mm zon çapı aralığında antibakteriyel aktivite sergilemiştir. Ayrıca tanen uygulamaları soğan kök ucu hücrelerinde doza bağımlı mitotik indeks azalmasına ve mitotik bölünme evrelerinde toksik etki göstererek ciddi bir inhibisyona neden olmuştur. Sonuç olarak, elde edilen veriler topraktaki C mineralizasyonunun farklı tanen kaynaklarından etkilendiğini ve bu tanen ekstraktlarının patojenlere karşı önemli antimikrobiyal aktiviteye ve A. cepa kök ucu hücrelerinde sitotoksik aktiviteye sahip olduğunu göstermiştir.

Anahtar Kelimeler

Karbon mineralizasyonu, tanen, antimikrobial aktivite, antimutajenik aktivite

Proje Numarası

FEF2006YL59

Kaynakça

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