Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) larvaları ve Centaurea depressa M. Bieb'in (Asteraceae) yara iyileştirici etkilerinin histolojik olarak araştırılması

Öz

Türkiye'nin Kronik yaralar, standart iyileşme sürecinin (genellikle 4-12 hafta) aksamasıyla karakterize, yaşam kalitesini düşüren ve sağlık sistemine önemli yük getiren bir klinik sorundur. Diyabet gibi metabolik hastalıklar kronik yaralara neden olabilir. Kronik yaraların tedavisinde modern tıbbi tedavilerin yanı sıra tamamlayıcı tedavi yaklaşımları da kullanılabilmektedir. Maggot Debridman Tedavisi (MDT), kronik yaralar için dünya çapında kullanılan etkili bir yöntemdir. MDT tedavisinde en yaygın kullanılan tür Lucilia sericata'dır. Larva salgı ürünleri antifungal, anti-enflamatuar ve antibakteriyel etkilere sahiptir. Bitkisel tedaviler de kronik yara bakımında kullanılmaktadır. Centaurea cinsinin birçok türü uzun zamandır geleneksel tıpta çeşitli amaçlar için kullanılmaktadır. Bu çalışmada, tedavi gruplarına L. sericata larvaları ve fitoterapötik ajan C. depressa bitki ekstresi uygulanmış ve yara iyileşmesi üzerindeki etkileri değerlendirilmiştir. Bu amaçla 4 grup oluşturulmuş ve diyabetli hayvanlara maggot ve bitki ekstraktı uygulanmıştır. Uygulamaların etkisini görmek için histolojik çalışmalar, yara ölçümleri, yara alanı ve kontraksiyon değerleri ölçülmüştür. Yara ölçümleri (alan ve kontraksiyon bulguları) ve histolojik bulgulara göre, bitki ekstraktı ve maggot tedavisinin yara iyileşme süreci üzerinde olumlu etkileri olmuştur. Maggot uygulaması bitki özü uygulaması ile karşılaştırıldığında maggot uygulamasının daha olumlu etkileri olduğu görülmüştür. Ancak birlikte kullanıldıklarında etkilerinin daha da arttığı gözlemlenmiştir. Çalışılan türlerin kronik yara modelindeki yara iyileştirme aktivitesinin daha önce karşılaştırmalı ve kombine olarak değerlendirilmemiş olması temel çıkış noktasıdır. Bu çalışma sonucunda C. depressa ve L. sericata'nın yara iyileşmesi üzerinde olumlu etkileri olduğu görülmüştür. Elde edilen verilerin integratif tıp uygulamalarına katkı sağlayabileceği düşünülmektedir.

Anahtar Kelimeler

• Maggot
• Lucilia sericata
• Centaurea depressa
• Diabetes mellitus
• yara iyileşmesi

Histological investigation of the wound healing effects of Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) larvae and Centaurea depressa M. Bieb. (Asteraceae)

Öz

Chronic wounds are a clinical problem characterised by disruption of the standard healing process (usually 4-12 weeks), reducing quality of life and placing a significant burden on the healthcare system. Metabolic diseases such as diabetes can cause chronic wounds. In the treatment of chronic wounds, complementary treatment approaches can be used alongside modern medical treatments. Maggot Debridement Therapy (MDT) is an effective method used worldwide for chronic wounds. The most commonly used species in MDT treatment is Lucilia sericata. Larval secretion products have antifungal, anti-inflammatory and antibacterial effects. Herbal treatments are also used in chronic wound care. Many species of the genus Centaurea have long been used in traditional medicine for various purposes. In this study, L. sericata larvae and the phytotherapeutic agent C. depressa plant extract were applied to treatment groups and their effects on wound healing were evaluated. For this purpose, 4 groups were created and maggot and plant extract were applied to the animals with diabetes. Histological studies, wound measurements, wound area and contraction values were measured to see the effect of the applications.According to wound measurements (area and contraction findings) and histological findings, plant extract and maggot treatment had positive effects on wound healing process. When maggot application was compared with plant extract application, it was seen that maggot application had more positive effects. However, it has been observed that their effects increase even more when used in combination.The main starting point is that the wound healing activity of the studied species in the chronic wound model has not been evaluated comparatively and combined before. As a result of this study, it was observed that C. depressa and L. sericata had positive effects on wound healing. It is thought that the data obtained may contribute to integrative medicine practices.

Anahtar Kelimeler

• Maggot
• Lucilia sericata
• Centaurea depressa
• Diabetes mellitus
• wound healing

Kaynakça

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