Carthamus tinctorius L. türüne ait bazı hat ve çeşitlerin karyotip analizi

Yıl 2018, , 1 - 9, 01.05.2018

Tuna Uysal , Betül Sena Tekkanat Ela Nur Şimşek Sezer , Rahim Ada , Meryem Bozkurt

https://doi.org/10.30616/ajb.357059

Cited By: 1

Öz

Bu çalışmada 31 Aspir (Carthamus tinctorius L.) çeşidine ait
kromozom sayıları ve karyomorfolojileri araştırılmıştır. Kromozom
sayımları, Carthamus'un aynı temel
kromozom sayılarına sahip olduğunu gösteren daha önceki raporları
doğrulamıştır.  Elde edilen sonuçlara
göre, kromozom sayıları tüm çeşitler için 2n = 24, x = 12 olarak tanımlanmış ve
aynı zamanda tümünün diploid kromozom sayısına sahip oldukları bulunmuştur. Karyotiplerde
metasentrik ve submetasentrik olan kromozomların baskın olduğu tespit
edilmiştir. Tüm çeşitleri karyolojik açıdan değerlendirmek ve C. tinctorius
çeşitleri arasındaki kromozomal değişikliklerini aydınlatmak için beş adet
kantitatif asimetri indeksi kullanılmıştır. Tüm karyotip analizleri ilk kez bu
raporda KAMERAM programı ile tanımlanmıştır. Çalışmada elde edilen bulguların Carthamus türünün genetik ve ıslah
çalışmalarına katkıda bulunmasını umuyoruz

Anahtar Kelimeler

Aspir, Karyomorfoloji, Asteraceae

Karyotype analysis of some lines and varieties belonging to Carthamus tinctorius L. species

Öz

In this paper, karyology of
thirty one accessions of Safflower (Carthamus tinctorius L.) were
investigated in terms of their chromosome numbers and karyomorphology. The
chromosomal counts confirmed the results of previous reports, that the Carthamus has same basic chromosome
numbers. According to our results discussed all accessions of chromosome
numbers have been identified 2n = 24, x=12 and also all have the diploid number
of chromosomes. We found predominance of chromosomes being metacentric and
sub-metacentric. Five quantitative asymmetry indices were used to evaluate our
karyological results in all species and elucidate the chromosomal alterations
of Carthamus tinctorius accessions.
All karyotyping analyses were described for the first time in this report via
KAMERAM programme. We hope that these findings would be contributed for Carthamus genetic and breeding studies.

Anahtar Kelimeler

Safflower, karyomorphology, Asteraceae

Kaynakça

• Anjali M, Srivastava AK (2009a). 3D somatic karyotype analyses in Carthamus tinctorius. Cytologia 73(4): 365-370.
• Anjali M, Srivastava AK (2009b). Karyotypic analysis of different populations of Carthamus tinctorius Linnaeus (Asteraceae). Comparative Cytogenetics 3(1): 51-55.
• Anjali M, Srivastava AK (2009c). The fine structure of safflower (Carthamus tinctorius L.) chromosomes as revealed by Pachytene analysis. Cytologia 74(3): 289-293.
• Anjali M, Srivastava AK (2012). Karyological studies in twelve accessions of Carthamus tinctoriusi. Caryologia 65(1): 1-6. Bowen CC (1956). Freezing by liquid carbon dioxide in making slides permanent. Stain Technology 31(2): 87-90.
• Cassini H (1819). Dictionaire de Sciences Naturelles, Paris. Cited by King R, Dawson HW, (1975) Cassini on Compositae. Oriole Editions, New York.
• Dajue L, Mündel HH (1996). Safflower (Carthamus tinctorius L.). Promoting the conservation and use of under utilized and neglected crops, 7. Institute of Plant Genetics and Crop Plant Reasarch, Gatersleben/International Plant Genetic Resources Institute, Rome, Italy.
• De Candolle AP, De Candolle A, Buek HW (1837). Prodromus Systematis Naturalis Regni Vegetabilis, Sive, Enumeratio Contracta Ordinum Generum Specierumque Plantarum Huc Usque Cognitarum, Juxta Methodi Naturalis Normas Digesta, 6: 611, Paris.
• Gyulai J 1996. Market outlook for safflower. P. 15 in Proceedings of North American Safflower Conference, Great Falls, Montana, January 17-18 (H.-H. Mündel, J. Braun and C. Daniels, eds.). Lethbridge, AB, Canada.
• Knowles PF (1958). Safflower. Advances in Agronomy 10: 289-323.
• Knowles PF (1988). Carthamus species relationships. A lecture in Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
• Levan A, Fredga K, Sandberg AA (1964). Nomenclature for centromeric position on chromosomes. Hereditas 52(2): 201-220.
• Li MX, Chen RY (1985). A suggestion on the standardization of karyotype analysis in plants. J Wuhan Bot Res 3(4): 297-302.
• López González G (1989). Acerca de la clasificación natural del género Carthamus L., sl. in Anales del Jardin Botanico de Madrid 47(1): 11-34).
• Peruzzi L, Leitch IJ, Caparelli KF (2009). Chromosome diversity and evolution in Liliaceae. Ann Bot Lond. 103(3): 459-475. Sehgal D, Raina, SN, Devarumath RM, Sasanuma T, Sasakuma T (2009). Nuclear DNA assay in solving issues related to ancestry of the domesticated diploid safflower (Carthamus tinctorius L.) and the polyploid (Carthamus) taxa, and phylogenetic and genomic relationships in the genus Carthamus L. (Asteraceae). Molecular phylogenetics and evolution 53(3): 631-644.
• Sheidai M, Sotoode M, Nourmohammadi Z (2009). Chromosome pairing and cytomixis in safflower (Carthamus tinctorius L., Asteraceae) cultivars. Cytologia 74(1): 43-53.
• Stebbins GL (1971). Chromosomal evolution in higher plants. Edward Arnold Ltd., London.
• Yazdani M, Sarrami M, Zeinali H (2013). Karyotype study of seven safflower (Carthamus tinctorius L.) populations native to Iran. The Nucleus 56(1): 31-36.
• Yenice N, Bayraktar N (1996). Yerli Aspir (Carthamus tinctorius L.) Hatlarında Karyotip Analizi. Tarım Bilimleri Dergisi 2(2): 81-86.