Cell growth inhibitory potential of Craterellus cornucopioides (L.) Pers. together with antioxidant and antimicrobial properties

Öz

Craterellus cornucopioides (L.) Pers which is also known as trumpet of death or horn of plenty, is a wild edible macrofungus. This study was conducted to elucidate the potential health beneficial properties of C. cornucopioides. Bioactive ingredients (phenolics, flavonoids, β-carotene and lycopene) and DPPH radical scavenging activities were determined. Additionally, cell growth inhibitory effects on HepG2 cells together with some bacteria were evaluated. Accordingly, water and methanol extracts contains 37.71±1.42 μg/mg and 13.78±1.60 μg/mg phenolic contents, respectively. Similarly, methanolic extracts have higher β-caroten and lycopene content as compared to aqueous extracts. In parallel with these antioxidants, methanolic extracts have also higher DPPH scavenging activity (IC50: 5.26±0.67 mg/ml). Besides, water extracts have higher flavonoid contents (2.13±0.06 μg/mg) then the methanolic extracts. C. cornucopioides has also an important cell growth inhibitory effects on HepG2 cell (IC50: 18.41±1.10 mg/ml for aqueous extracts and IC50: 3.14±1.07 mg/ml for methanolic extracts). Moreover, both extracts were effective on six different bacteria tested. As a result, this study indicates that C. cornucopioides could reduce the cellular oxidative stress because of its high antioxidant ingredients, inhibit the growth of pathogen microrganisms and have some degree of cell growth inhibitory potential at least to the HepG2 cells.

Anahtar Kelimeler

• Craterellus cornucopioides
• antioxidant
• antibacterial
• cytotoxicity
• HepG2

Antioksidan ve antimiktobiyal özellikleri ile birlikte Craterellus cornucopioides (L.) Pers. türünün hücre büyümesi baskılama potansiyeli

Öz

Ölüm trompeti veya bolluk boynuzu olarak da bilinen Craterellus cornucopioides (L.) Pers, yenilebilir bir makrofungustur. Bu çalışma Craterellus cornucopioides (L.) Pers mantarının sağlık açısından yararlı özelliklerini açığa çıkarmak amacıyla yapılmıştır. Çalışmada ilgili mantarın biyoaktif içerikleri (fenolikler, flavonoidler, β-karoten ve likopen) ve DPPH radikal süpürücü aktiviteleri belirlenmiştir. Ek olarak, HepG2 hücreleri ve bazı bakteri türleri üzrine hücre büyümesini baskılayıcı etkileri değerlendirilmiştir. Buna göre, su ve metanol ekstraktları sırasıyla 37.71±1.42 μg/mg ve 13.78±1.60 μg/mg fenolik içeriğe sahiptir. Benzer şekilde, metanol ekstraktları sulu ekstrelere kıyasla daha yüksek β-karoten ve likopen içeriğine sahiptir. Bu antioksidanlara paralel olarak, metanol ekstraktları da DPPH süpürme aktivitesine daha fazla sahiptir (ICso: 5.26±0.67 mg/ml). Ayrıca su ekstraktları, metanol ekstraklarına göre daha yüksek flavonoid içeriğine (2.13±0.06 μg/mg) sahiptir. C. cornucopioides, HepG2 hücresi üzerinde de önemli bir hücre büyümesi engelleyici etkiye sahiptir (IC50: su ekstratları için 18.41±1.10 mg/ml ve metanol ektraktları için IC50: 3.14±1.07 mg/ml). Buna ek olarak, her iki ekstrakt da test edilen altı farklı bakteri türü üzeine etkili olmuştur. Sonuç olarak, bu çalışma C. cornucopioides'in yüksek antioksidan bileşenleri nedeniyle hücresel oksidatif stresi azaltabildiğini, patojen mikroorganizmalarının büyümesini inhibe edebileceğini ve en azından HepG2 hücrelerine bir miktar hücre büyümesi inhibitör potansiyeline sahip olduğunu göstermektedir.

Anahtar Kelimeler

• Craterellus cornucopioides
• antioksidan
• antibakteriyel
• sitotoksisite
• HepG2

Kaynakça

1. Alves MJ, Ferreira ICFR, Martins A, Pintado M (2012). Antimicrobial activity of wild mushroom extracts against clinical isolates resistant to different antibiotics. J Appl Microbiol 113(2):466-475.
2. Barros L, Ferreira MJ, Queiros B, Ferreira ICFR, Baptista P (2007). Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 103:413-419.
3. Chang ST, Buswell JA (1996). Mushroom nutriceuticals. World J Microb Biot 12: 473-476.
4. Chang ST, Wasser SP (2012). The role of culinary medicinal mushrooms on human welfare with a pyramid model for human health. Int J Med Mushrooms 14:95-134.
5. Cheung LM., Cheung PCK, Ooi VEC (2002). Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 81: 249-255
6. Gan CH, Nurul AB, Asmah R (2013). Antioxidant analysis of different types of edible mushrooms (Agaricus bisporous and Agaricus brasiliensis). Int Food Res J 20(3): 1095-1102.
7. Jagadish LK, Krishnan VV, Shenbhagaraman R, Kaviyarasan V (2009). Comparative study on the antioxidant, anticancer and antimicrobial property of Agaricus bisporus (J. E. Lange) Imbach before and after boiling. African J Biot 8(4): 654-661.
8. Karamac M, Amarowichz R, Weidner S, Abe S, Shahidi F (2002). Antioxidant activity of rye caryopses and embryos extracts. Czech J Food Sci 20: 209-214.

9. Kozarski M, Klaus A, Jakovljevic D, Todorevic N, Vunduk J, Petrovic P (2015). Antioxidants of edible mushrooms. Molecules 20: 19489-19525.
10. Manivannan E, Kothai R, Arul B, Rajaram S (2011). In-vitro antioxidant properties of Sterculia foetida Linn. Res J Pharm Biol Chem Sci 2:43-52.
11. Mattila P, Suonpaa K, Piironen V (2000). Functional properties of edible mushrooms. Nutrition 16(7-8):694-696.
12. Pal J, Ganguly S, Tahsin KS, Acharya K (2010). In vitro free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (Mont.) Singer. Indian J Exp Biol 48:1210‑1218.
13. Pan HH, Yu XT, Li T, Wu HL, Jiao CW, Cai MH, Li XM, Xie YZ, Wang Y, Peng T (2013). Aqueous extract from a Chaga medicinal mushroom, Inonotus obliquus (higher Basidiomycetes), prevents herpes simplex virus entry through inhibition of viral-induced membrane fusion. Int J Med Mushrooms 15: 29-38.
14. Palacios I, Lozano M, Moro C, D’Arrigo M, Rostagno MA, Martinez JA, García-Lafuente A, Guillamón E, Villares A (2011). Antioxidant properties of phenolic compounds occurring in edible mushrooms. Food Chem 3: 674-678
15. Sadi G, Emsen B, Kaya A, Kocabaş A, Çınar Ş, Kartal Dİ (2015). Cytotoxicity of some edible mushrooms extracts overliver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties. Pharmacogn Mag, 1:6-18
16. Smania EFA, Delle Monache F, Smania Jr A, Yunes RA, Cuneo RS (2003). Antifungal activity of sterols and triterpenes isolated from Ganoderma annulare. Fitoterapia 74:375-377.
17. Smania Jr A, Delle Monache F, Smania EFA, Cuneo RS (1999). Antibacterial activity of steroidal compounds isolated from Ganoderma applanatum (Pers.) Pat. (Aphyllophoromycetideae) fruit body. Int J Med Mushrooms 1:325-330.
18. Türkoğlu A, Duru ME, Mercan N, Kıvrak İ, Gezer K (2007). Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murril. Food Chem 101: 267-273.
19. Yen GC, Chen HY (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. J of Agri Food Chem 43: 27–32.
20. Yıldız O, Can Z, Laghari AQ, Şahin H, Malkoç M (2015). Wild edible mushrooms as a natural source of phenolics and antioxidants. J Food Biochem 39:148–154
21. Taga MS, Miller EE, Pratt DE (1984) Chia seeds as a source of natural lipid antioxidants. J Am Oil Chem Soc 61(5):928-931.