In vitro axillary shoot regeneration from cotyledonary node of Bishop’s weed (Trachyspermum ammi L.)

Öz

Bishop’s seed (Trachyspermum ammi L.) is a vital neutraceutial plant used as spice and also used as medicinal plant for curing different ailments and diseases of humans and animals. The study presents the in vitro regeneration of Bishop’s seed using cotyledonary node explant taken from in vitro germinated seeds. All mediums used in this study were comprised of MS (0.44%) medium having 3.0%sucrose and 0.65% agar with pH of 5.8. Expalnts were inoculated on MS medium enriched with 0.10-1.60 mg/L Thidiazuron (TDZ) alone or in combination with 0.10 mg/L IBA (Indole-3-butyric acid) for seven weeks under 16/8 light photoperiod provided with with cool-white fluorescent lamps. Thereafter, explants were sucultured on MS medium without any plant growth regulators (PGRs) for five weeks more under similar culture conditions. Results revealed 100% shoot regeneration frequency with 42.85-100.00%. The shoot count and shoot length ranged 3.43-19.40 and 1.04-2.74 cm respectively. The highest shoot count (19.40) and shoot length (2.74 cm) were observed on Ms medium enriched with 0.20 mg/L TDZ and 0.20 mg/L TDZ+0.10 mg/L IBA respectively. Relatively low rooting was recorded on IBA containing medium with low adaptation percentage of plantlets in pots containing peat moss.

Anahtar Kelimeler

• Bishop’s weed
• Cotyledonary node
• In vitro
• Thidiazuron

Bishop otunun (Trachyspermum ammi L.) kotiledon boğum eksplantından in vitro aksil sürgün rejenerasyonu

Öz

Bishop otu (Trachyspermum ammi L.) çok önemli baharat olarak kullanılan nutrasötik bitki olup, aynı zamanda insan ve hayvanların farklı rahatsızlık ve hastalıklarını tedavi için kullanılan tıbbi bitkidir. Bu çalışmada Bishop otunun in vitro çimlenmiş fidelerden elde edilen kotiledon boğum eksplantlar kullanılarak in vitro çoğaltım yapılmıştır. Tüm çalışmalarda %0.44% MS, %3.0 sukroz ve %0.65 agar ile 5,8 pH içeren besi ortamı kullanılmıştır. Eksplantlar 0.10-1.60 mg/L Thidiazuron (TDZ) veya 0.10-1.60 mg/L TDZ+ 0,1 mg/L Indole-3-butyric acid (IBA) içeren besi oratmlarında kültüre alınarak yedi hafta boyunca soğuk beyaz floresan lambalar ile desteklenmiş 16/8 saat ışık fotoperyotında bekletilmiştir. Daha sonra eksplantlar her hangi hormone içeremeyen MS besi ortamına alt kültüre alınarak benzer koşullarda beş hafta boyunca bekletilmiştir. Sürgün rejenerasyon yüzdesi 100 olurken kallus rejenerasyon yüzdesi ise %42,85-100,00% arasında kaydedilmiştir. Sürgün sayısı ve sürgün uzunluğu ise sırasıyla 3,43-19,40 ve 1,04-2,74 cm olarak kaydedilmiştir. En fazla sürgün sayısı (19,40) ve sürgün uzunluğu (2,74 cm) sırasıyla 0,20 mg/L TDZ ve 0,20 mg/L TDZ+0,10 mg/L IBA içeren ortamında elde edilmiştir. Genel olarak IBA içeren ortamlarında düşük oranda köklendirme kaydedilirken, saksılarda aktarıldığında da düşük oranda adaptasyon sağlanmıştır.   

Anahtar Kelimeler

• Bishop otu
• in vitro
• kotiledon boğum
• tidiazuron

Kaynakça

1. Aasim M, Sahin-Demirbag N, Khawar KM, Kendir H, Özcan S (2011). Direct Axillary hoot Regeneration From The Mature Seed Explant Of The Hairy Vetch (Vicia villosa Roth)". Archives of Biological Sciences 63(3): 757-762.
2. Ashraf M, Orooj A (2006). Salt stress effects on growth, ion accumulation and seed oil concentration in an arid zone traditional medicinal plant ajwain (Trachyspermum ammi [L.] Sprague). Journal of Arid Environments 64(2): 209-220. Bairwa R, Sodha RS, Rajawat BS (2012). Trachyspermum ammi. Pharmacognosy Reviews 6: 56-60.
3. Chaudhary D, Madanpotra S, Jaiwal R, Saini A, Kumar P, Pawan JK (2007).. Agro bacterium tumefaciens-mediated high frequency genetic transformation of an Indian Cowpea (Vigna unguiculata L.Walp) cultivar and transmission of transgene into progeny. Plant Science 172: 692-700.
4. Jain N, Sharma V, Ramawat KG (2011). Therapeutic potentials of medicinal plants traditionally used during postpartum period and their molecular targets. Journal of Ecobiotechnology 3: 30.
5. Jeet K, Devi N, Narender T, Sunil T, Lalit S, Raneev T (2012). Trachyspermum ammi (Ajwain): Comprehensive Review. International Research Journal of Pharmacy 3: 133-138.
6. Karataş M, Aasim M (2014). Efficient in vitro regeneration of medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell). Pakistan Journal of Agricultural Sciences 51(3): 667-672.
7. Kendir H, Sahin-Demirbag N, Khawar KM, and Aasim M (2008). In vitro plant regeneration from Narbon Vetch (Vicia narbonensis L.) using cotyledonary node explants. African Journal of Biotechnology 7(14): 2491-2494.
8. Koca A, Aasim M (2015). Establishment of efficient micropropagation system in Bishop’s weed (Trachyspermum ammi L) using mature seed explant. Journal of Animal and Plant Sciences 25 (2): 478-484.

9. Kwon PI, Junheon K, Sang-Gil L (2007). Nematicidal activity of plant essential oils and components from ajwain (Trachyspermum ammi), allspice (Pimentadioica) and litsea (Litseacubeba) essential oils against pine wood nematode (Bursaphelenchus xylophilus). Journal of Nematolgy 39: 275-279.
10. Mathew N, Bhattacharya SM, Perumal V, Muthuswamy K (2008). Antifilarial lead molecules ısolated from Trachyspermum ammi. Molecules 13: 2156-2168.
11. Moghaddam M, Khaleghi MSN, Ghasemi PA, Mehdizadeh L, Ghaderi Y. (2015). Variation in essential oil composition and antioxidant activity of cumin (Cuminum cyminum L.) fruits during stages of maturity. Industrial Crops and Products 70: 163-169.
12. Moosavi SG, Seghatoleslami MJ, Jouyban Z, Ansarinia E, Moosavi SA (2015). Response morphological traits and yield of ajowan (Carum copticum) to water deficit stress and nitrogen fertilizer. In: Biological Forum. Research Trend, p. 293.
13. Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
14. Niazian M, Noori SAS, Galuszka P, Tohidfar M, Mortazavian SMM (2017b). Genetic stability of regenerated plants via indirect somatic embryogenesis and indirect shoot regeneration of Carum copticum L. Industrial Crops and Products 97: 330-337.
15. Niazian M, Sadat NSA, Tohidfar M, Mortazavian SMM (2017a). Essential oil yield and agro-morphological traits in some Iranian ecotypes of ajowan (Carum copticum L.). Journal of Essential Oil Bearing Plants 20(4): 1151-1156.
16. Nomani M, Noori SAS, Tohidfar M, Ramshini H (2021). Regeneration of Trachyspermum ammi L. Sprague ecotypes via indirect somatic embryogenesis using hypocotyl and epicotyl explants. Indian Journal of Experimental Biology 59(04): 263-269.
17. Nomani M, Tohidfar M (2021). Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos. Journal of Genetic Engineering and Biotechnology 19(1): 1-10.
18. Platel K, Srinivasan K (2001). Studies on the influence of dietary spices on food transit time in experimental rats. Nutrition Research 21: 1309-1314.
19. Ramaswamy S, Sengottuvelu S, Haja SS, Jaikumar S, Saravanan R, Prasadkumar C, Sivakumar T (2010).
20. Gatroprotective activity of ethanolic extract of Trachyspermum ammi fruit. International Journal of Pharmacy and Biological Sciences 1: 1-15.
21. Sharifi‐Rada M, Sharifi‐Radb M, Sharifi‐Radc R (2013). Antibacterial activity of Trachyspermum copticum leaves extract against resistant bacteria to antibiotics. Phytoscience 1: 9‐12.
22. Singh VK, Singh S, Singh DK (2003). Pharmacological effects of spices. In Recent Progress in Medicinal Plants. Phytochemistry and Pharmacology. Stadium Press, Houston Texas, USA pp. 321-353.
23. Snedecor GW, Cochran WG, 1967. Statistical Methods. The Iowa State University Press, USA .
24. Şahin-Demirbag N, Kendir H, Khawar KM, Aasim M (2008). In vitro plant regeneration from Hungarian vetch (Vicia pannonica Crantz) using cotyledonary node explants. Biotechnology & Biotechnological Equipments 22(4): 929-932.
25. Teymourian H, Ebrahimi MA, Tohidfar M, Farsaloon N, Zarinpanjeh N (2017). In vitro plantlet regeneration from callus culture of Trachyspermum copticum. Plant Tissue Culture and Biotechnology 27(1): 13-20.
26. Thangam C, Dhananjayan R (2003). Antiinflammatory potential of the seeds of Carum copticum Linn. Indian Journal of Pharmacology 34: 388‐391.
27. Tripathi L, Tripathi JN (2003). Role of biotechnology in medicinal plants. Tropical Journal of Pharmaceutical Research 2: 243.
28. Velazhahan R, Vijayanandraj S, Vijayasamundeeswari A, Paranidharan V, Samiyappan R, Iwamoto T (2010). Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill. Structural analysis and biological toxicity of degredation product of aflatoxin G1. Food Control 21: 719-725