Effects of Suillus collinitus (Fr.) Kuntze extracts on genotoxicity and proliferation of human lymphocytes

Yıl 2019, Cilt: 3 Sayı: 2, 59 - 63, 01.11.2019

Bugrahan Emsen Asuman Türel Yasin Uzun

https://doi.org/10.30616/ajb.605495

Cited By: 1

Öz

Cultivated or wild edible
mushroom species have traditionally been used by humans for medical purposes
for many years. Edible mushrooms have the potential to show different
activities due to the numerous bioactive components they contain. In
particular, some mushroom species whose regulatory properties have been
identified on human immunity are of interest in the scientific world.
Considering these characteristics of edible mushroom species, in the present
study, it was examined the effects of Suillus
collinitus (Fr.) Kuntze, an important edible mushroom species, on human
peripheral lymphocytes. For this purpose, acetone and water extracts
were obtained from S. collinitus and
the effects of these extracts on genotoxicity and proliferation of human
lymphocytes were tested by chromosome aberration (CA), micronucleus (MN),
nuclear division index (NBI) and mitotic index (MI) analyses. When genotoxicity
analyses were examined, it was found that none of the tested extract
applications (1-100 mg/L) did not change the CA and MN frequencies
statistically (p > 0.05) compared
to the negative control group. Proliferation analyses showed that only the
maximum concentration (100 mg/L) application of
acetone extract of S. collinitus decreased
the NBI and MI ratio of the cells at a level of p < 0.05 compared to the negative control group.
The obtained results revealed that the acetone and water extracts of S. collinitus, especially the
applications at concentrations of 1-50 mg/L, did not show any genotoxic or
cytotoxic activity on lymphocytes involved in the human immune system.

Anahtar Kelimeler

Cytotoxicity, Genotoxicity, Lymphocyte, Suillus collinitus

Destekleyen Kurum

Karamanoglu Mehmetbey University Scientific Research Projects Commission

Proje Numarası

06-YL-19

Teşekkür

This work was supported by Karamanoglu Mehmetbey University Scientific Research Projects Commission [grant number 06-YL-19].

Suillus collinitus (Fr.) Kuntze ekstraktlarının insan lenfositlerinin genotoksisitesi ve proliferasyonu üzerindeki etkileri

Öz

Kültüre edilmiş veya yabani
yenilebilir mantar türleri yıllardır insanlar tarafından geleneksel olarak
tıbbi amaçlı kullanılmaktadır. Yenilebilir mantarlar içerdikleri kendilerine
özgü çok sayıda biyoaktif bileşen sayesinde farklı aktiviteler gösterme potansiyeline
sahiptir. Özellikle insan bağışıklığı üzerinde düzenleyici özellikleri tespit
edilen bazı mantar türleri bilim dünyasında dikkat çekmektedir. Yenilebilir
mantar türlerinin bu özellikleri göz önünde bulundurularak, mevcut çalışmada
önemli yenilebilir bir mantar türü olan Suillus collinitus (Fr.) Kuntze’un insan periferal
lenfositleri üzerindeki etkileri incelenmiştir. Bu amaçla, S. collinitus’tan aseton ve su ekstraktları elde
edilmiş ve bu ekstraktların insan lenfositlerinin genotoksisitesi ve proliferasyonu
üzerindeki etkileri, kromozom aberasyonu (KA), mikronükleus (MN), nükleer
bölünme indeksi (NBİ) ve mitotik indeks (Mİ) analizleri ile test edilmiştir.
Genotoksisite analizleri incelendiğinde, test edilen ekstrakt uygulamalarının
(1-100 mg/L) hiçbirinin negatif kontrol grubuna kıyasla KA
ve MN frekanslarını istatistiksel (p >
0.05) olarak değiştirmediği
tespit edilmiştir. Proliferasyon analizleri ise yalnızca S. collinitus’un aseton ekstraktının maksimum konsantrasyonlu (100
mg/L) uygulamasının, hücrelerin NBİ ve Mİ oranını negatif kontrol grubuna
kıyasla p <
0.05 düzeyinde düşürdüğünü göstermiştir. Elde
edilen sonuçlar, S. collinitus’un
aseton ve su ekstraktlarının özellikle 1-50 mg/L konsantrasyonlu
uygulamalarının, insan bağışıklık sisteminde görevli lenfositler üzerinde
herhangi bir genotoksik veya sitotoksik aktivite göstermediğini ortaya
çıkarmıştır.

Anahtar Kelimeler

Sitotoksisite, Genotoksisite, Lenfosit, Suillus collinitus

Proje Numarası

06-YL-19

Kaynakça

• Akata I, Ergönül B, Kalyoncu F (2012). Chemical compositions and antioxidant activities of 16 wild edible mushroom species grown in Anatolia. International Journal of Pharmacology 8(2): 134-138.
• Algar E, Dagar V, Sebaj M, Pachter N (2011). An 11p15 imprinting centre region 2 deletion in a family with Beckwith Wiedemann Syndrome provides insights into imprinting control at CDKN1C. Plos One 6(12): e29034.
• Benítez G, Molero-Mesa J, González-Tejero MR (2017). Gathering an edible wild plant: food or medicine? A case study on wild edibles and functional foods in Granada, Spain. Acta Societatis Botanicorum Poloniae 86(3): 3550.
• Breitenbach J, Kränzlin F (1995). Fungi of Switzerland, Vol. 4. Lucerne: Verlag Mykologia.
• Chirapongsatonkul N, Mueangkan N, Wattitum S, U-taynapun K (2019). Comparative evaluation of the immune responses and disease resistance of Nile tilapia (Oreochromis niloticus) induced by yeast β-glucan and crude glucan derived from mycelium in the spent mushroom substrate of Schizophyllum commune. Aquaculture Reports 15: 100205.
• De Silva DD, Rapior S, Fons F, Bahkali AH, Hyde KD (2012). Medicinal mushrooms in supportive cancer therapies: an approach to anti-cancer effects and putative mechanisms of action. Fungal Diversity 55(1): 1-35.
• De Silva DD, Rapior S, Sudarman E, Stadler M, Xu J, Aisyah Alias S, Hyde KD (2013). Bioactive metabolites from macrofungi: ethnopharmacology, biological activities and chemistry. Fungal Diversity 62(1): 1-40.
• Desjardin DE, Wood MG, Stevens FA (2014). California Mushrooms: The Compherensive Identification Guide. London: Timber Press. Emsen B, Guven B (2019). Activities of two edible macrofungi, Coprinus comatus and Leucoagaricus leucothites in human lymphocytes: cytogenetic and biochemical study. Plant Biosystems epub ahead of print:1-8.
• Emsen B, Guven B, Kaya A (2019). Antioxidant and antigenotoxic potential of Lycoperdon molle Pers., a wild edible mushroom. KSU Journal of Agricultural and Nature 22(5): 724-732.
• Ergönül PG, Ergönül B, Kalyoncu F, Akata I (2012). Fatty acid compositions of five wild edible mushroom species collected from Turkey. International Journal of Pharmacology 8(5): 463-466.
• Froufe HJC, Abreu RMV, Ferreira ICFR (2011). QCAR models to predict wild mushrooms radical scavenging activity, reducing power and lipid peroxidation inhibition. Chemometrics and Intelligent Laboratory Systems 109(2): 192-196.
• Gabory A, Attig L, Junien C (2009). Sexual dimorphism in environmental epigenetic programming. Molecular and Cellular Endocrinology 304(1-2): 8-18.
• Gill BS, Navgeet, Mehra R, Kumar V, Kumar S (2018). Ganoderic acid, lanostanoid triterpene: a key player in apoptosis. Investigational New Drugs 36(1): 136-143.
• Heleno SA, Barros L, Sousa MJ, Martins A, Ferreira ICFR (2010). Tocopherols composition of Portuguese wild mushrooms with antioxidant capacity. Food Chemistry 119(4): 1443-1450. Hsieh T-C, Wu J. (2011). Suppression of proliferation and oxidative stress by extracts of Ganoderma lucidum in the ovarian cancer cell line OVCAR-3. International Journal of Molecular Medicine 28(6): 1065-1069.
• Jordan M (1995). The Encyclopedia of Fungi of Britain and Europe. Devon: David & Charles Book Co.
• Kalač P (2013). A review of chemical composition and nutritional value of wild-growing and cultivated mushrooms. Journal of the Science of Food and Agriculture 93(2): 209-218.
• Khatua S, Ghosh S, Acharya K (2017). Chemical composition and biological activities of methanol extract from Macrocybe lobayensis. Journal of Applied Pharmaceutical Science 7(10): 144-151.
• Kumar S, Gupta SK, Sharma PK (2012). Recent developments in targeted drug delivery system for crossing bloodbrain barrier: a review. International Journal of Pharmacy and Pharmaceutical Sciences 4(2): 36-41.
• Nguyet TMN, Lomunova M, Le BV, Lee JS, Park SK, Kang JS, Kim YH, Hwang I (2018). The mast cell stabilizing activity of Chaga mushroom critical for its therapeutic effect on food allergy is derived from inotodiol. International Immunopharmacology 54: 286-295.
• Pala SA, Wani AH, Bhat MY (2013). Ethnomycological studies of some wild medicinal and edible mushrooms in the Kashmir Himalayas (India). International Journal of Medicinal Mushrooms 15(2): 211-220.
• Rahman MA, Abdullah N, Aminudin N (2018). Evaluation of the antioxidative and hypo-cholesterolemic effects of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), in ameliorating cardiovascular disease. International Journal of Medicinal Mushrooms 20(10): 961-969.
• Randhawa K, Shri R (2018). Comparison of antioxidant and anticholinesterase activities of selected Pleurotus species (Agaricomycetes) from India. International Journal of Medicinal Mushrooms 20(8): 739-748.
• Sangeetha K, Senthilkumar G, Panneerselvam A, Sathammaipriya N (2019). Cultivation of oyster mushroom (Pleurotus sp) using different substrates and evaluate their potentials of antibacterial and phytochemicals. International Journal of Research in Pharmaceutical Sciences 10(2): 997-1001.
• Su S, Ding X, Fu L, Hou Y (2019). Structural characterization and immune regulation of a novel polysaccharide from Maerkang Lactarius deliciosus Gray. International Journal of Molecular Medicine 44(2): 713-724.
• Valverde ME, Hernández-Pérez T, Paredes-López O (2015). Edible mushrooms: improving human health and promoting quality life. International Journal of Microbiology 2015: 1-14.
• Vaz JA, Ferreira ICFR, Tavares C, Almeida GM, Martins A, Helena Vasconcelos M (2012). Suillus collinitus methanolic extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line. Food Chemistry 135(2): 596-602.
• Wang G, Zhang X, Maier SE, Zhang L, Maier RJ (2019). In vitro and in vivo inhibition of Helicobacter pylori by ethanolic extracts of lion’s mane medicinal mushroom, Hericium erinaceus (Agaricomycetes). International Journal of Medicinal Mushrooms 21(1): 1-11.
• Wang X-M, Zhang J, Wu L-H, Zhao Y-L, Li T, Li J-Q, Wang Y-Z, Liu H-G (2014). A mini-review of chemical composition and nutritional value of edible wild-grown mushroom from China. Food Chemistry 151: 279-285.
• Zengin G, Sarikürkzü C, Aktümsek A, Uysal S, Ceylan R, Anwar F, Solak M (2015). A Comparative fatty acid compositional analysis of different wild species of mushrooms from Turkey. Emirates Journal of Food and Agriculture 27(7): 532-536.
• Zhao X, Fang L, Liu D, Lai C, Zhang Y, Zhou A, Xie J (2018). A glucogalactomannan isolated from Agaricus bisporus induces apoptosis in macrophages through the JNK/Bim/caspase 3 pathway. Food & Function 9(9): 4771-4780.
• Cite this article: Emsen B, Türel A, Uzun Y (2019). Effects of Suillus collinitus (Fr.) Kuntze extracts on genotoxicity and proliferation of human lymphocytes. Anatolian Journal of Botany 3(2): 59-63.