Phenolic screening and biological activities of Chenopodium botrys L. extracts

Yıl 2021, Cilt: 5 Sayı: 2, 78 - 83, 15.11.2021

Ela Nur Şimşek Sezer , Tuna Uysal

https://doi.org/10.30616/ajb.890324

Öz

The genus Chenopodium is a genus that includes over 200 species and contains annual or perennial herbaceous flowering plant species scattered almost worldwide. While species belonging to the Chenopodium genus are used to treat various diseases, modern pharmaceutical research has revealed their strong anticancer, antifungal and antibacterial potential. In this study, some biological effects of Chenopodium botrys (methanol and ethanol) extracts were evaluated by helping some analytical approaches. The antioxidant and cytotoxic potentials of the extracts were revealed. In this study, 4 phenolic components in the extracts were determined with the HPLC technique and to determine the antioxidant activity of the plant extracts TPC (total phenolic content), RPA (reducing power activity), and DPPH (2,2′-diphenyl-1-picrylhydrazyl) methods were used. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cytotoxic effect of the extracts on the HL60 cell line. The results showed that both extracts have important properties that should be evaluated, and especially the ethanolic extract is more effective than the methanolic extract in all aspects (TPC, antioxidant capacity, and cytotoxicity). As a conclusion, it is clear that this plant needs to be investigated further for the isolation and characterization of new and effective phytochemicals. We hope that this study could be a precursor for future studies.

Anahtar Kelimeler

Antioxidant, HPLC, HL60, MTT, phenolic

Proje Numarası

Proje No: 19401125

Kaynakça

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