Bishop otunun (Trachyspermum ammi L.) kotiledon boğum eksplantından in vitro aksil sürgün rejenerasyonu

Yıl 2021, Cilt: 5 Sayı: 2, 134 - 137, 15.11.2021

Asuman Koca Muhammad Aasım

https://doi.org/10.30616/ajb.988363

Öz

Bishop otu (Trachyspermum ammi L.) çok önemli baharat olarak kullanılan nutrasötik bitki olup, aynı zamanda insan ve hayvanların farklı rahatsızlık ve hastalıklarını tedavi için kullanılan tıbbi bitkidir. Bu çalışmada Bishop otunun in vitro çimlenmiş fidelerden elde edilen kotiledon boğum eksplantlar kullanılarak in vitro çoğaltım yapılmıştır. Tüm çalışmalarda %0.44% MS, %3.0 sukroz ve %0.65 agar ile 5,8 pH içeren besi ortamı kullanılmıştır. Eksplantlar 0.10-1.60 mg/L Thidiazuron (TDZ) veya 0.10-1.60 mg/L TDZ+ 0,1 mg/L Indole-3-butyric acid (IBA) içeren besi oratmlarında kültüre alınarak yedi hafta boyunca soğuk beyaz floresan lambalar ile desteklenmiş 16/8 saat ışık fotoperyotında bekletilmiştir. Daha sonra eksplantlar her hangi hormone içeremeyen MS besi ortamına alt kültüre alınarak benzer koşullarda beş hafta boyunca bekletilmiştir. Sürgün rejenerasyon yüzdesi 100 olurken kallus rejenerasyon yüzdesi ise %42,85-100,00% arasında kaydedilmiştir. Sürgün sayısı ve sürgün uzunluğu ise sırasıyla 3,43-19,40 ve 1,04-2,74 cm olarak kaydedilmiştir. En fazla sürgün sayısı (19,40) ve sürgün uzunluğu (2,74 cm) sırasıyla 0,20 mg/L TDZ ve 0,20 mg/L TDZ+0,10 mg/L IBA içeren ortamında elde edilmiştir. Genel olarak IBA içeren ortamlarında düşük oranda köklendirme kaydedilirken, saksılarda aktarıldığında da düşük oranda adaptasyon sağlanmıştır.   

Anahtar Kelimeler

Bishop otu, in vitro, kotiledon boğum, tidiazuron

Destekleyen Kurum

YOK

Proje Numarası

YOK

Teşekkür

YOK

In vitro axillary shoot regeneration from cotyledonary node of Bishop’s weed (Trachyspermum ammi L.)

Öz

Bishop’s seed (Trachyspermum ammi L.) is a vital neutraceutial plant used as spice and also used as medicinal plant for curing different ailments and diseases of humans and animals. The study presents the in vitro regeneration of Bishop’s seed using cotyledonary node explant taken from in vitro germinated seeds. All mediums used in this study were comprised of MS (0.44%) medium having 3.0%sucrose and 0.65% agar with pH of 5.8. Expalnts were inoculated on MS medium enriched with 0.10-1.60 mg/L Thidiazuron (TDZ) alone or in combination with 0.10 mg/L IBA (Indole-3-butyric acid) for seven weeks under 16/8 light photoperiod provided with with cool-white fluorescent lamps. Thereafter, explants were sucultured on MS medium without any plant growth regulators (PGRs) for five weeks more under similar culture conditions. Results revealed 100% shoot regeneration frequency with 42.85-100.00%. The shoot count and shoot length ranged 3.43-19.40 and 1.04-2.74 cm respectively. The highest shoot count (19.40) and shoot length (2.74 cm) were observed on Ms medium enriched with 0.20 mg/L TDZ and 0.20 mg/L TDZ+0.10 mg/L IBA respectively. Relatively low rooting was recorded on IBA containing medium with low adaptation percentage of plantlets in pots containing peat moss.

Anahtar Kelimeler

Bishop’s weed, Cotyledonary node, In vitro, Thidiazuron

Proje Numarası

YOK

Kaynakça

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