Pelargonium quercetorum Agnew’in toplam flavonoid, fenolik ve antioksidan aktiviteleri: in vivo ve in vitro yetiştirilen bitkinin karşılaştırılması

Yıl 2022, Cilt: 6 Sayı: 2, 69 - 74, 15.11.2022

İbrahim Kuru Pınar Orcan Mehmet Fırat

https://doi.org/10.30616/ajb.1131225

Öz

Günümüzde, fitokimyasal özelliğe sahip doğal bileşikler yan etkilerinin olmaması nedeniyle insan dostu ilaçlar olarak kabul edilirler. Bu nedenle bitkilerde de bulunan antioksidan bileşiklere verilen önem her geçen gün artarak devam etmektedir. Pelargonium L'Hér. ex Aiton taksonları da solunum yolu enfeksiyonları, dizanteri, karaciğer şikayetleri ve ishal tedavisi gibi hastalıklarda iyileştirici olarak kullanılmaktadır. Bitki doku ve hücre kültürü yöntemleri, polifenoller gibi bazı aktif metabolitlerin üretilmesi için iyi bir araçtır. Bu yöntemler ile sekonder metabolit üretiminin teşvik edildiği ve antioksidan kapasitede değişikliklere neden olduğu da bilinmektedir. Bu kapsamda araştırmamızda, Pelargonium quercetorum Agnew bitkisi in vivo (rizom ve toprak üstü kısım) ve in vitro şartlarda yetiştirilerek toplam fenolik, flavonoit ve antioksidan kapasitelerinin belirlenmesi amaçlanmıştır. Elde edilen sonuçlar incelendiğinde, en yüksek fenolik ve flavonoit içerik in vitro sürgün ekstresinde; en düşük fenolik ve flavonoit içerik ise rizom ekstresinde tespit edilmiştir. Araştırmamızda toplam antioksidan aktiviteyi belirlemek amacıyla 3 farklı yöntem (DPPH, ABTS, CUPRAC) kullanılmıştır. Antioksidan aktivite sonuçları genel olarak değerlendirildiğinde, her üç yöntemde de aktivite sıralamasının “in vitro sürgün ˃ in vivo toprak üstü ˃ in vivo rizom” şeklinde olduğu gözlenmiştir. DPPH yönteminde bitki ekstreleri pozitif kontrol olarak kullanılan BHT’ye göre, ABTS yönteminde ise BHA’ya göre daha iyi aktivite göstermiştir. Ayrıca toplam fenolik-flavonoit içerik ile antioksidan aktivite arasında pozitif korelasyon olduğu görülmüştür. Bu çalışmadan elde edilen veriler, P. quercetorum bitkisinin antioksidan aktiviteye sahip olduğunu, doğal kaynaklı antioksidan madde arayışında çalışmamızın bir basamak olacağı düşünülmektedir.

Anahtar Kelimeler

Antioksidan, in vitro, in vivo, P. quercetorum

Total flavonoid, phenolic and antioxidant activities of Pelargonium quercetorum Agnew: Comparison of in vivo and in vitro grown plant

Öz

Nowadays, natural compounds with phytochemical properties are considered human-friendly drugs because they do not have side effects. Therefore, the importance given to antioxidant compounds, which are also found in plants, continues to increase day by day. Pelargonium L'Hér. ex Aiton taxa are also used as curative in diseases such as respiratory tract infections, dysentery, liver complaints and diarrhea treatment. Plant tissue and cell culture techniques are a good tool for the production of some active metabolites such as polyphenols. It is also known that with these methods, secondary metabolite production is promoted and there are also changes in antioxidant capacity. In this context, it was aimed to determine the total phenolic, flavonoid and antioxidant capacities of Pelargonium quercetorum Agnew plant by growing in vivo (rhizome and above-ground part) and in vitro conditions. When the results obtained were examined, the highest phenolic and flavonoid content was found in the shoot extract in vitro; the lowest phenolic and flavonoid content was determined in the rhizome extract. In our study, 3 different methods (DPPH, ABTS, CUPRAC) were used to determine the total antioxidant activity. When the antioxidant activity results were evaluated in general, it was observed that the order of activity in all three methods was "in vitro shoot ˃ in vivo above ground ˃ in vivo rhizome". In the DPPH method, plant extracts showed better activity than BHT, which was used as a positive control, and better than BHA in the ABTS method. In addition, a positive correlation was observed between total phenolic-flavonoid content and antioxidant activity. The data obtained from this study, it is thought that the plant P. quercetorum has antioxidant activity, and our study will be a step in the search for natural origin antioxidants.

Anahtar Kelimeler

Antioxidant, in vitro, in vivo, P. quercetorum

Kaynakça

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