Determination of efficient DNA recovery method in Pyracantha coccinea for use in forensic botany

Yıl 2025, Cilt: 9 Sayı: 1, 6 - 10

Tuğba Ünsal Sapan , Elvan Acarkan , Nurdan Sandıkçı

https://doi.org/10.30616/ajb.1540607

Öz

Forensic Botany aims to uncover the connection between crime scenes, perpetrators, or victims using plant materials. Since the presence of metabolites in plants varies among different species, DNA isolation methods should be adjusted specifically for each plant species and even each plant tissue. In this study, the objective was to determine the most suitable DNA isolation method for the fruits of firethorn (Pyracantha coccinea M.Roem.) growing in Türkiye without using liquid nitrogen. In the study, the fruits of Firethorn were divided into two groups as fresh (12 pieces) and dried (12 pieces), which each fruit was subjected to different homogenization methods using a single sample for each analysis. After completing the DNA isolation of the samples, the DNA quantities were measured to determine which method yielded optimal results. The highest DNA quantity of 4.80 ng/µl was obtained from dried fruits homogenized using a mortar and tissue lyser with beads, while the lowest DNA quantity of 0.13 ng/µl was obtained from fresh fruits homogenized only with a kitchen blender. The findings of the study indicate that for both fresh and dried fruits, homogenization with a mortar and tissue lyser with beads would result in a high recovery of DNA from plant materials. This study provides an optimization for DNA isolation in forensic cases where the Firethorn plant may be involved.

Anahtar Kelimeler

Forensic Sciences, Forensic Botany, Plant DNA Extraction, Homogenization Methods, Pyracantha Coccinea (Firethorn)

Etik Beyan

No acknowledgment and/or disclaimers applicable

Destekleyen Kurum

Uskudar University

Teşekkür

The authors would like to thank Üsküdar University for the laboratory opportunity

Pyracantha coccinea'da etkili DNA geri kazanım yönteminin belirlenmesi ve adli botanikte kullanımı

Öz

Adli Botanik disiplini bitki materyallerini kullanarak olay yeri, fail veya kurban arasındaki bağlantıyı ortaya çıkarmayı hedeflemektedir. Bitkilerde bulunan ikincil metabolitlerin varlığı farklı bitki türlerinde değişiklik gösterdiğinden DNA izolasyon yöntemleri her bitki türüne hatta her bitki dokusuna özel olarak ayarlanmalıdır. Çalışmamızda, Türkiye’de yetişen Ateş Dikeni (Pyracantha coccinea M.Roem.) bitkisinin meyveleri için sıvı nitrojen kullanılmadan en uygun DNA izolasyon metodunun belirlenmesi amaçlanmıştır. Çalışmamızda, ateş dikeni bitkisinin meyveleri taze (12 adet) ve kurutulmuş (12 adet) olarak iki gruba ayrılmış ve her analizde tek meyve kullanılarak farklı homojenizasyon yöntemlerine tabi tutulmuştur. DNA izolasyonu tamamlanan örneklerin DNA miktarları ölçülerek hangi metodun optimum sonuç verdiği belirlenmiştir. En yüksek DNA miktarı 4,80 ng/µl olarak havan ve boncuklu doku öğütücü ile homojenize edilen kurutulmuş meyvelerden elde edilirken, en düşük DNA miktarı ise 0,13 ng/µl olarak yalnızca mutfak robotu ile homojenize edilen taze meyvelerden elde edilmiştir. Çalışmamızın bulguları hem taze hem de kurutulmuş meyveler için, homojenizasyonun havan ve boncuklu doku öğütücü ile gerçekleştirilmesi halinde bitki materyallerinden yüksek miktarda DNA geri kazanımı olacağı yönündedir. Yaptığımız çalışma, Ateş Dikeni bitkisinin dahil olabileceği adli vakalarda DNA izolasyonu için optimizasyon sunmaktadır.

Anahtar Kelimeler

Adli bilimler, adli botanik, bitki DNA izolasyonu, homojenizasyon metotları, Pyracantha coccinea (ateş dikeni)

Kaynakça

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