Determination of antimicrobial and anti-MRSA activities of Cladonia foliacea extracts

Yıl 2025, Cilt: 9 Sayı: 2, 108 - 113

Erdoğan Güneş , Hatice Esra Akgül

https://doi.org/10.30616/ajb.1672104

Öz

This study aims to determine the antimicrobial activity of methanol and ethanol extracts of Cladonia foliacea (Huds.) Willd. against pathogenic standard microorganisms and the anti-MRSA activity against clinical MRSA strains. Minimum Inhibitory Concentrations (MIC) were determined by the broth microdilution method. All strains were exposed to concentrations of extracts between 12.5-0.006 mg/ml. Methanol and ethanol extracts were found to have antibacterial activity against standard bacterial strains at doses of 0.01-6.25 mg/ml. The most effective strain for both extracts was the Bacillus cereus strain with an MIC value of 0.01 mg/ml. It was observed that the extracts had a higher effect on gram-positive bacteria than gram-negative bacteria. The MIC values of ethanol and methanol extract against Candida albicans yeast strain were calculated as 0.39 mg/ml and 0.78 mg/ml, respectively. In addition, it was found in the study that both extracts showed very high anti-MRSA activity against all clinical MRSA strains at doses of 0.01-6.25 mg/ml. It can be said that this species can be a natural source of antimicrobial agents and can be used as an alternative in the treatment of MRSA strains.

Anahtar Kelimeler

Antimicrobial activity , Anti-MRSA , Lichen , Methicillin resistance Staphylococcus aureus

Cladonia foliacea ekstraktlarının antimikrobiyal ve anti-MRSA aktivitelerinin belirlenmesi

Öz

Bu çalışmanın amacı Cladonia foliacea (Huds.) Willd.’in metanol ve etanol ekstraktlarının patojen standart mikroorganizmalara karşı antimikrobiyal aktivitesini ve klinik MRSA suşlarına karşı anti-MRSA aktivitesinin belirlenmesidir. Minimum İnhibisyon Konsantrasyonları (MİK) sıvı mikrodilüsyon metoduyla saptanmıştır. Tüm suşlar ekstraktların 12,5-0,006 mg/ml arasındaki konsantrasyonlarına maruz bırakılmıştır. Metanol ve etanol ekstraktlarının, standart bakteri suşlarına karşı 0.01-6.25 mg/ml dozlarda antibakteriyal aktivite sahip olduğu tespit edilmiştir. Her iki ekstraktın da en etkili olduğu suş 0.01 mg/ml MİK değeriyle Bacillus cereus suşu olmuştur. Ekstraktların gram pozitif bakterilere gram negatif bakterilerden daha yüksek etki gösterdiği görülmüştür. Candida albicans maya suşuna karşı etanol ve metanol ekstraktının MİK değerleri sırasıyla 0.39 mg/ml ve 0.78 mg/ml olarak hesaplanmıştır. Ayrıca çalışmada her iki ekstraktın klinik MRSA suşlarının tamamına karşı 0.01-6.25 mg/ml dozlarında oldukça yüksek anti-MRSA aktivitesi gösterdikleri bulunmuştur. Sonuç olarak bu türün antimikrobiyal ajanların doğal bir kaynağı olabileceği ve MRSA suşlarının tedavisinde alternatif olarak kullanılabileceği söylenebilir.

Anahtar Kelimeler

Antimikrobiyal aktivite , Anti-MRSA , Liken , Metisilin dirençli Staphylococcus aureus.

Kaynakça

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