Detection of Norovirus, Rotavirus and Astrovirus Antigens in Hand Swabs and Stool Specimens of Employees in Dairy Processing Plants

Yıl 2021, , 393 - 397, 30.12.2021

Hatice Aydogan Oğuz Gürsoy Mehmet Kale

https://doi.org/10.24323/akademik-gida.1050764

Öz

In this study, swab and stool samples were obtained from employees (n=47) working in five dairy processing plants located in Burdur province (Turkey) and the district of Bucak to determine the prevalence of norovirus (NoV), rotavirus (RoV) and astrovirus (AsV) antigens by the enzyme-linked immunosorbent assay (ELISA) technique. Swab samples were obtained from both hands (palm, upper part, sides of fingers and fingernail tips) of employees. In a questionnaire, participants were asked to provide information regarding their gender, age, education level, smoking status, hygiene education status, habits of glove use during working as well as whether they had had digestive problems such as diarrhea, vomiting and abdominal pain in the period of study. Results of the stool analyses indicated that NoV antigen was present in an employee of a dairy processing plant, which was not participated in any hygiene education. AsV and RoV antigens were absent in swap and stool samples of employees. According to results of the questionnaire, 42 of the 47 employees frequently used gloves while 4 employees used gloves rarely. It was determined that 7 of 47 staff was not in participated any hygiene education, and one of those 7 staff did not use gloves during working. It can be concluded that hygiene in the working environment and personnel in these dairy processing plants were sufficient and appropriate from viral perspective. However, detection of NoV antigen in stool sample of a staff of a dairy processing plant shows that there is high viral contamination potential for employees of dairy processing plants. Thus, hygiene education in food processing plants including dairy plants to prevent possible viral infections and outbreaks and prevent to loss of workforce is extremely important.

Anahtar Kelimeler

Norovirus, Rotavirus, Astrovirus, ELISA, Dairy plant, Norovirus, Rotavirus, Astrovirus, ELISA, Dairy plant

Destekleyen Kurum

Coordinatorship of Scientific Research Projects of Burdur Mehmet Akif Ersoy University

Proje Numarası

Project Number: 0268-YL-15

Teşekkür

The authors are grateful to Dr. Hasbi Sait Saltık for his technical contributions during laboratory analyses. This article was produced from the Master’s Thesis prepared by Hatice Aydogan under the supervisions of Dr. O. Gursoy and Dr. M. Kale. This research was supported by Coordinatorship of Scientific Research Projects of Burdur Mehmet Akif Ersoy University (Project Number: 0268-YL-15).

Süt İşletmeleri Çalışanlarının El ve Gaita Örneklerinde Astrovirüs, Norovirüs ve Rotavirüs Antijenlerinin Belirlenmesi

Öz

Bu çalışmada Burdur il merkezi ve Bucak ilçesindeki 5 farklı süt ve süt ürünleri işletmesinin üretim bölümünde çalışan 47 personelin ellerinden (el ayası, el üstü, el parmak araları ve el tırnak uçları) alınan swap örneklerinde ve gaitalarında norovirüs (NoV), rotavirüs (RoV) ve astrovirüs (AsV), antijenlerinin varlığının enzim bağlı immünosorbent analizi (ELISA) ile belirlenmesi amaçlanmıştır. Ayrıca araştırmaya katılan personelin yaş, eğitim düzeyi, sigara içme durumu, hijyen eğitimi alıp almadıkları ve eldiven kullanımı ile ilgili bilgiler ile çalışmanın yapıldığı dönemde ishal, kusma ve karın ağrısı gibi sindirim sistemi semptomları olup olmadığı anket ile belirlenmiştir. Çalışma sonucunda 1 personelin gaitasında NoV antijeni tespit edilirken incelenen diğer el swap örnekleri ile gaita örneklerinde AsV, NoV ve RoV virüs antijenlerine rastlanmamıştır. Yapılan anket çalışmasında çalışan 47 personelden 42’sinin sıklıkla, 4’ünün de nadiren eldiven kullandığı belirlenmiştir. Çalışmaya katılan 47 personelden 7’sinin hijyen eğitimi almadığı, hijyen eğitimi almayanlardan 1 kişinin de eldiven kullanmadığı tespit edilmiştir. Yine gaitasında NoV antijeni tespit edilen personelin hijyen eğitimi almadığı görülmüştür. Sonuç olarak, çalışma kapsamında olan gıda işletmelerinde çalışan personel ile çalışma ortamlarının virolojik açıdan hijyenik koşullar bakımından uygun ve yeterli olduğu söylenebilir. Ancak bir işletme çalışanının gaitasında NoV antijenin tespit edilmesi işletme personeline ve personelin üretim zincirinde özellikle ambalajlama sırasında temas ettiği gıda maddelerine viral bulaşma potansiyelinin yüksek olduğunu göstermektedir. Bu nedenle gıda işletmelerinde hijyen eğitimi muhtemel enfeksiyon ve salgınların önlenmesi ve iş gücü kayıplarının engellemesi açısından son derece önemlidir.

Anahtar Kelimeler

Norovirüs, Rotavirüs, Astrovirüs, ELISA, Süt işletmesi, Norovirüs, Rotavirüs, Astrovirüs, ELISA, Süt işletmesi

Proje Numarası

Project Number: 0268-YL-15

Kaynakça

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