Polycyclic Aromatic Hydrocarbons (PAHs) Contamination Levels in Some Commercial Olive Oils Sold on the Markets in Giresun, Türkiye

Yıl 2024, , 86 - 95, 04.09.2024

Onur Basançelebi

https://doi.org/10.24323/akademik-gida.1542570

Öz

Olive oil is widely consumed worldwide and well-known for its health beneficial effects. However, polycyclic aromatic hydrocarbons (PAHs) contamination in olive oils has been a serious concern and knowledge regarding PAH concentrations in olive oils is scarce. The aim of this study was the determination and evaluation of PAH contamination levels in olive oils in the market. In this study, commercial olive oils (n=14) available in the markets of Giresun (Türkiye), including extra virgin and riviera olive oil samples, were investigated. Samples were analyzed by a gas chromatography unit coupled with a mass spectrometer. Significant amounts of PAHs were determined in all olive oil samples. The PAH contents of the samples varied between 0.04 µg kg-1 and 4637.13 µg kg-1 with the mean content of 264.61 µg kg-1. Regarding benzo[a]pyrene, 64.28% of the samples was incompatible with the EU requirements. None of the samples were compatible with the EU requirements in terms of PAH4 components (benzo[a]pyrene, benzo(a)anthracene, chrysene, and benzo[b]fluoranthene). Results showed that commercial olive oils studied in this research might contain PAHs categorized under potential carcinogens.

Anahtar Kelimeler

Gas chromatography-mass spectrometer (GC-MS), Olive oil, Polycyclic aromatic hydrocarbons (PAHs), Benzo[a]pyrene, PAH4

Giresun’da Satılan Bazı Ticari Zeytinyağlarının Polisiklik Aromatik Hidrokarbon (PAH) Kontaminasyon Düzeyleri

Öz

Zeytinyağı dünya çapında yaygın olarak tüketilmektedir ve sağlığa yararları ile bilinmektedir. Ancak zeytinyağındaki polisiklik aromatik hidrokarbon (PAH) kontaminasyonu ciddi bir endişe kaynağıdır ve bu yağların PAH konsantrasyonlarına ilişkin bilgi azdır. Bu çalışmanın amacı, piyasada bulunan zeytinyağlarındaki PAH kontaminasyon düzeylerinin belirlenmesi ve değerlendirilmesidir. Bu çalışmada Giresun ili marketlerinden rastgele seçilen sızma zeytinyağı ve riviera zeytinyağı örnekleri (n=14) incelenmiştir. Numuneler, kütle spektrometresi ile birleştirilmiş gaz kromatografisi ünitesi ile belirlenmiştir. Yağ örneklerinin tamamında önemli miktarda PAH kalıntısı tespit edilmiştir. PAH tespit edilen örneklerde PAH miktarları 0.04 µg kg-1 ile 4637.13 µg kg-1 (ortalama 264.61 µg kg-1) arasında değişmektedir. Benzo[a]piren ile ilgili olarak numunelerin %64.28'i AB gereklilikleriyle uyumlu çıkmamıştır. Zeytinyağı örneklerinin hiçbiri PAH4 bileşenleri (benzo[a]piren, benzo(a)antrasen, krizen ve benzo[b]floranten) açısından AB gerekliliklerine uygun çıkmamıştır. Sonuçlar, bu araştırmada incelenen ticari zeytinyağlarının potansiyel kanserojenler kategorisinde yer alan PAH'ları içerebileceğini göstermiştir.

Anahtar Kelimeler

Gaz kromatografisi-kütle spektrometresi (GC-MS), Zeytinyağı, Polisiklik aromatik hidrokarbonlar (PAH, Benzo[a]piren, PAH4

Kaynakça

• [1] Estruch, R., Ros, E., Salas-Salvadó, J., Covas, M.I., Corella, D., Arós, F., Martínez-González, M.A. (2013). Primary prevention of cardiovascular disease with a Mediterranean diet. New England Journal of Medicine, 368(14), 1279-1290.
• [2] Köseoğlu, O., Sevim, D., Kadiroğlu, P. (2016). Quality characteristics and antioxidant properties of Turkish monovarietal olive oils regarding stages of olive ripening. Food Chemistry, 212, 628-634.
• [3] Bulotta, S., Celano, M., Lepore, S.M., Montalcini, T., Pujia, A., Russo, D. (2014). Beneficial effects of the olive oil phenolic components oleuropein and hydroxytyrosol: focus on protection against cardiovascular and metabolic diseases. Journal of Translational Medicine, 12(1), 219.
• [4] Fabiani, R. (2016). Anti-cancer properties of olive oil secoiridoid phenols: A systematic review of in vivo studies. Food and Function, 7(10), 4145-4159.
• [5] Kaddoumi, A., Denney Jr, T.S., Deshpande, G., Robinson, J.L., Beyers, R.J., Redden, D.T., Praticò, D., Kyriakides, T.C., Lu, B., Kirby, A.N., Beck, D.T., Merner, N D. (2022). Extra-virgin olive oil enhances the blood–brain barrier function in mild cognitive impairment: A randomized controlled trial. Nutrients, 14(23), 5102.
• [6] International Olive Council, (2018). Olive oil production and consumption up by 1 million tonnes in the last 25 years. IOC News. April 19, 2018. https://www.internationaloliveoil.org/1071-olive-oil-production-and-consumption-up-by-1-million-tonnes-in-the-last-25-years/. (Accessed January 22, 2020).
• [7] Zaidi, A.J., Ahsan, H., Munshi, A.B. (2021). A review on cancer probability in human beings due to environmental impact of polycyclic aromatic hydrocarbons (PAHs) and remediation. Pakistan Journal of Scientific and Industrial Research Series A: Physical Sciences, 64(3), 275-286.
• [8] Gharbi, I., Moret, S., Chaari, O., Issaoui, M., Conte, L.S., Lucci, P., Hammami, M. (2017). Evaluation of hydrocarbon contaminants in olives and virgin olive oils from Tunisia. Food Control, 75, 160-166.
• [9] Ekner, H., Dreij, K., Sadiktsis, I. (2022). Determination of polycyclic aromatic hydrocarbons in commercial olive oils by HPLC/GC/MS–Occurrence, composition and sources. Food Control, 132, 108528.
• [10] Ifegwu, O.C., Anyakora, C. (2015). Polycyclic aromatic hydrocarbons: part I. Exposure. Advances in Clinical Chemistry, 72, 277-304.
• [11] Bertoz, V., Purcaro, G., Conchione, C., Moret, S. (2021). A review on the occurrence and analytical determination of PAHs in olive oils. Foods, 10(2), 324.
• [12] United States Environmental Protection Agency (EPA), “Dose-response assessment for assessing health risks associated with exposure to hazardous air pollutants,” https://www.epa.gov/fera/dose-response-assessment-assessing-health-risks-associated-exposure-hazardous-air-pollutants, (Accessed February 6, 2024).
• [13] European Commission (EC), “Standard IARC classification,” https://ec.europa.eu/health/scientific_committees/opinions_layman/en/electromagnetic-fields/glossary/ghi/iarc-classification.htm, (Accessed February 6, 2024).
• [14] Hussain, K., Hoque, R.R., Balachandran, S., Medhi, S., Idris, M.G., Rahman, M., Hussain, F.L. (2018). Monitoring and risk analysis of PAHs in the environment. Handbook of Environmental Materials Management, 1-35.
• [15] Aydın, Ö.Ş., Şahan, Y. (2018). Bazı et türlerinde polisiklik aromatik hidrokarbon oluşumuna farklı pişirme yöntemlerinin etkisi. Akademik Gıda, 16(4), 387-394.
• [16] Çıtak, D. (2006). Zeytinyağı ve Pirina Yağındaki BAP Kirliliğinin HPLC ile Tespiti. Yüksek Lisans Tezi. Pamukkale Üniversitesi Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, Denizli.
• [17] Arvanaghi, H.R., Aminfar, Y., Sadeghan, A.A., Dolatabadi, N. (2017). Phytoremediation of polycyclic aromatic hydrocarbons (PAHs): air-plant-soil interactions. Journal of Biological and Environmental Sciences, 10, 178-184.
• [18] Wei, L., Lv, J., Zuo, P., Li, Y., Yang, R., Zhang, Q., Jiang, G. (2024). The occurrence and sources of PAHs, oxygenated PAHs (OPAHs), and nitrated PAHs (NPAHs) in soil and vegetation from the Antarctic, Arctic, and Tibetan Plateau. Science of The Total Environment, 912, 169394.
• [19] da Silva Junior, F.C., Felipe, M.B.M.C., de Castro, D.E.F., da Silva Araújo, S.C., Sisenando, H.C.N., de Medeiros, S.R.B. (2021). A look beyond the priority: A systematic review of the genotoxic, mutagenic, and carcinogenic endpoints of non-priority PAHs. Environmental Pollution, 278, 116838.
• [20] Fromberg, A., Højgård, A., Duedahl-Olesen, L. (2007). Analysis of polycyclic aromatic hydrocarbons in vegetable oils combining gel permeation chromatography with solid-phase extraction clean-up. Food Additives and Contaminants, 24(7), 758-767.
• [21] Çevik, S., Aydın, S., Sermet, O.S., Özkan, G., Karacabey, E. (2017). Optimization of olive oil extraction process by response surface methodology. Akademik Gıda, 15(4), 337-343.
• [22] Aydar, A.Y., Bağdatlıoğlu, N., Köseoğlu, O. (2017). Effect of ultrasound on olive oil extraction and optimization of ultrasound-assisted extraction of extra virgin olive oil by response surface methodology (RSM). Grasas y Aceites, 68(2), 189-189.
• [23] Franco, M.N., Galeano-Díaz, T., López, Ó., Fernández-Bolaños, J.G., Sánchez, J., De Miguel, C., Martín-Vertedor, D. (2014). Phenolic compounds and antioxidant capacity of virgin olive oil. Food Chemistry, 163, 289-298.
• [24] Köylüoğlu, F., Özkan, G. (2012). Yardımcı katkı maddeleri kullanımının zeytinyağı verim ve kalite parametreleri üzerine etkisi. Electronic Journal of Food Technologies, 7(3), 32-45.
• [25] Kıralan, S.S., Toptancı, İ., Öncül Abacıgil, T., Ramadan, M. F. (2020). Phthalates levels in olive oils and olive pomace oils marketed in Turkey. Food Additives & Contaminants: Part A, 37(8), 1332-1338.
• [26] Büyükgök, E.B., Ötleş, S. (2011). Metaller ve zeytinyağının etkileşimi. Zeytin Bilimi, 2(2), 75-82.
• [27] Karaca, H., Yemiş, O. (2008). Mikotoksin kontaminasyonu: Zeytin ve Ürünlerinde Toksin Riski. I. Ulusal Zeytin Öğrenci Kongresi, 17-18.
• [28] García-Reyes, J.F., Ferrer, C., Gómez-Ramos, M.J., Fernández-Alba, A.R., Molina-Díaz, A. (2007). Determination of pesticide residues in olive oil and olives. Trac Trends in Analytical Chemistry, 26(3), 239-251.
• [29] Baloğlu, Z., Bayrak, A. (2006). Polisiklik aromatik hidrokarbon (PAH)'lardan benzo(a)pirenin sızma, riviera ve prina zeytinyağlarında belirlenmesi. Gıda Dergisi, 31(5), 239-251.
• [30] Şekeroğlu, G., Fadıloğlu, S., Göğüş, F. (2006). Bitkisel Yağlarda Benzo(a)piren Miktarının Yüksek Basınçlı Sıvı Kromatografisi ile Belirlenmesi. 24-26 Mayıs Türkiye 9. Gıda Kongresi, Bolu, 9, 855-858.
• [31] Elaridi, J., Fakhro, M., Yamani, O., Dimassi, H., Othman, H., Attieh, Z. (2020). GC–MS analysis of polycyclic aromatic hydrocarbons in bottled olive oil marketed in Lebanon. Toxicological Research, 36, 211-220.
• [32] Taghvaee, Z., Piravivanak, Z., Rezaei, K., Faraji, M. (2016). Determination of polycyclic aromatic hydrocarbons (PAHs) in olive and refined pomace olive oils with modified low temperature and ultrasound-assisted liquid–liquid extraction method followed by the HPLC/FLD. Food Analytical Methods, 9, 1220-1227.
• [33] Peng, B., Dong, Q., Li, F., Wang, T., Qiu, X., Zhu, T. (2023). A systematic review of polycyclic aromatic hydrocarbon derivatives: Occurrences, levels, biotransformation, exposure biomarkers, and toxicity. Environmental Science and Technology, 57(41), 15314-15335.
• [34] Moret, S., Conte, L.S. (2002). A rapid method for polycyclic aromatic hydrocarbon determination in vegetable oils. Journal of Separation Science, 25(1‐2), 96-100.
• [35] Scientific Committee on Food, (2002). Opinion of the Scientific Committee on Food on the risks to human health of polycyclic aromatic hydrocarbons in food; SCF/CS/CNTM/PAH/29 ADD1 Final; European Commission: Brussels, Belgium.
• [36] European Food Safety Authority, (2008). Polycyclic aromatic hydrocarbons in food. Scientific opinion of the panel on contaminants in the food chain. The EFSA Journal, 6(8), 1-114.
• [37] European Commission (EC) (2007). “Report on experts participating in Task 3.2.12,” Collection of occurrence data on polycyclic aromatic hydrocarbons in food 2007, http://ec.europa.eu/food/food/chemicalsafety/contaminants/scoop_3-2-12_final_report_pah_en.pdf (Accessed February 6, 2024).
• [38] van Der Wielen, J.C.A., Jansen, J.T.A., Martena, M.J., De Groot, H.N., In't Veld, P.H. (2006). Determination of the level of benzo[a]pyrene in fatty foods and food supplements. Food Additives and Contaminants, 23(7), 709-714.
• [39] Pupin, A.M., Toledo, M.C.F. (1996). Benzo(a)pyrene in olive oils on the Brazilian market. Food Chemistry, 55(2), 185-188.
• [40] Krajian, H., Odeh, A. (2018). Levels of 15+ 1 EU priority polycyclic aromatic hydrocarbons in different edible oils available in the Syrian market. Polycyclic Aromatic Compounds, 38(4), 369-378.
• [41] Rascón, A.J., Azzouz, A., Ballesteros, E. (2018). Multiresidue determination of polycyclic aromatic hydrocarbons in edible oils by liquid-liquid extraction-solid-phase extraction-gas chromatography-mass spectrometry. Food Control, 94, 268-275.
• [42] Ju, H., Kim, B., Kim, J., Baek, S.Y. (2020). Development of candidate reference method for accurate determination of four polycyclic aromatic hydrocarbons in olive oil via gas chromatography/high-resolution mass spectrometry using 13C-labeled internal standards. Food Chemistry, 309, 125639.
• [43] Shi, L.K., Zhang, D.D., Liu, Y.L. (2016). Incidence and survey of polycyclic aromatic hydrocarbons in edible vegetable oils in China. Food Control, 62, 165-170.
• [44] Pratt, M.M., John, K., MacLean, A.B., Afework, S., Phillips, D.H., Poirier, M.C. (2011). Polycyclic aromatic hydrocarbon (PAH) exposure and DNA adduct semi-quantitation in archived human tissues. International Journal of Environmental Research and Public Health, 8(7), 2675-2691.
• [45] Ramesh, A., Archibong, A.E., Niaz, M.S. (2010). Ovarian susceptibility to benzo [a] pyrene: tissue burden of metabolites and DNA adducts in F-344 rats. Journal of Toxicology and Environmental Health, Part A, 73(23), 1611-1625.
• [46] Rodríguez-Acuña, R., Pérez-Camino, M.D.C., Cert, A., Moreda, W. (2008). Sources of contamination by polycyclic aromatic hydrocarbons in Spanish virgin olive oils. Food Additives and Contaminants, 25(1), 115-122.
• [47] Šcimko, P., Khunova, V., Šcimon, P., Hruba, M. (1995). Kinetics of sunflower oil contamination with polycyclic aromatic hydrocarbons from contaminated recycled low density polyethylene film. International Journal of Food Science and Technology, 30(6), 807-812.
• [48] Moret, S., Conte, L.S. (2000). Polycyclic aromatic hydrocarbons in edible fats and oils: occurrence and analytical methods. Journal of Chromatography A, 882(1-2), 245-253.
• [49] Susamcı, E., Ötleş, S., Dıraman, H. (2017). Sofralık zeytin ve zeytinyağı teknolojisi. Zeytincilik Araştırma Enstitüsü Müdürlüğü Bassaray Matbaası 1. Basım.
• [50] Palamutoğlu, R., Sarıçoban, C., Kasnak, C. (2014). Polisiklik aromatik hidrokarbonlar (PAH) ve et ürünlerinde oluşumu, Gıda Teknolojileri Elektronik Dergisi, 9(3), 47-57.
• [51] Cejpek, K., Hajšlová, J., Kocourek, V., Tomaniová, M., Cmolik, J. (1998). Changes in PAH levels during production of rapeseed oil. Food Additives and Contaminants, 15(5), 563-574.
• [52] Larsson, B.K., Eriksson, A.T., Cervenka, M. (1987). Polycyclic aromatic hydrocarbons in crude and deodorized vegetable oils. Journal of the American Oil Chemists’ Society, 64(3), 365-370.
• [53] Teixeira, V.H., Casal, S., Oliveira, M.B.P. (2007). PAHs content in sunflower, soybean and virgin olive oils: Evaluation in commercial samples and during refining process. Food Chemistry, 104(1), 106-112.