Biofilm Producing Microorganisms in Dairy Industry and Prevention of Biofilm Formation
Yıl 2011,
Cilt: 9 Sayı: 4, 12 - 16, 01.08.2011
Zübeyde Öner
Zeynep Ölmez
Öz
In this study, the formation of biofilms in dairy industry was investigated. Biofilm samples were collected from the equipments of various dairy plants in Burdur and Isparta cities in Turkey. A biofilm layer was formed with isolated strains under laboratory conditions, and several disinfection procedures were applied to these biofilms. To remove the biofilms formed on stainless steel plates, six different disinfectants were used at different concentrations and impact times. Following microbial loads were found in samples collected from biofilms; total mesophilic aerob bacteria
Kaynakça
- [1] Mittelman, M.W., 1998. Structure and functional characteristics of bacterial biofilms in fluid processing operations. J.Dairy Sci. 81: 2760-2764.
- [2] Koutzayiotis, D., 1992. Bacterial biofilms in milk pipelines. S. Afr. J. Dairy Sci. 64: 19-22.
- [3] Flint, S.H., Bremer, P.J., Brooks, J.D., 1997. Biofilms in dairy manufacturing plant-description, current concerns and methods of control. Biofouling 11: 81-97.
- [4] Bremer, P., Suzanne Fillery, J., James McQuillan, A., 2006. Laboratory scale Clean-In-Place (CIP) studies on the effectiveness of different caustic and acid wash steps on the removal of dairy biofilms. Int. J. Food Microbiol. 106: 254-262.
- [5] Austin, J.W., Berferon, G., 1995. Development of bacterial biofilms in dairy processing lines. J.Dairy Res. 62: 509-519.
- [6] Amy, C., Wong, L., 1998. Biofilms in food processing environments. J. Dairy Sci. 81: 2765- 2770.
- [7] Halkman, A.K., 2005. Gıda Mikrobiyolojisi Uygulamaları. Başak Matbaacılık ve Tanıtım Hizmetleri Ltd. Şti. Ankara. 358p.
- [8] Sharma, M., Anand, S.K., 2002. Characterization of constitutive microflora of biofilms in dairy processing lines. Food Microbiol. 19: 627-636.
- [9] Bore, E., Langsrud, S., 2005. Characterization of microorganisms isolated from dairy industry after cleaning and fogging disinfection with alkyl amine and peracetic acid. J. of Appl. Microbiol. 98: 96-105.
- [10] Brown, M.R., Allison, D.G., Gilbert, P., 1988. Resistance of bacterial biofilms to antibiotics: a growth rate related effect. J. Antimicrob. Chemotherapy 22: 777-783.
- [11] Brading, M.G., Jass, J., Lappin-Scott, H., 2003. Dynamics of Bacterial Biofilm Formation. Microbial Biofilms. Edited by H.M.Lappin-Scott and J.W.Costerton Cambridge University pres.301p.
- [12] Frank, J.F., Koff, R.A., 1990. Surface adherent growth of Listeria monocytogenes is associated with increased resistance to surfactant sanitizers and heat. J. Food Protect. 53: 550-554.
- [13] Deibel, V., 2005. Biofilms. Internet Journal of Food Safety 1: 6-7.
- [14] Parkar, S.G., Flint, S.H., Brooks, J.D., 2003. Physiology of biofilms of thermophilic bacillipotential consequences for cleaning. J. Ind. Microbiol. Biotech. 30: 553-560.
- [15] Andersson, A., Rönner, U., Granum, P., 1995. What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens? Int. J. Food Microbiol. 28: 145-155.
- [16] Becker, H., Schaller, G., Wiese, W., Terplan, G., 1994. Bacillus cereus in infant foods and dried milk products. Int. J. Food Microbiol. 23: 1-15.
- [17] Shaheen, R., Svensson, B., Andersson, M.A., Christiansson, A., Salkinoja-Salonen, M., 2010. Persistence strategies of Bacillus cereus spores isolated from dairy silo tanks. Food Microbiol. 27: 347-355.
- [18] Wijnands, L.M., Dufrenne, J.B., Rombouts, F.M., Veld, P.H., Van Leusden, F.M., 2006. Prevalence of potentially pathogenic Bacillus cereus in food commodities in the Nether. J. Food Protec. 69: 2587-2594.
- [19] Banyko, J., Vyletelova, M., 2009. Determining the source of Bacillus cereus and Bacillus licheniformis isolated from raw milk, pasteurized milk and yoghurt. Letters in Appl. Microbiol. 48: 318-323.
- [20] Bartoszewicz, M., Hansen, B.M., Swiecicka, I., 2008. The members of the Bacillus cereus group are commonly present contaminants of fresh and heat-treated milk. Food Microbiol. 25: 588-596.
- [21] Svensson, B., Ekelund, K., Ogura, H., Christiansson, A., 2004. Characterization of Bacillus cereus isolated from milk silo tanks at eight different dairy plants. Int. Dairy J. 14: 17-27.
- [22] Svensson, B., Monthan, A., Shaheen, R., Andersson, A., Salkinoja-Salonen, M., Christiansson, A., 2006. Occurrence of emetic toxin producing Bacillus cereus in the dairy production chain. Int. Dairy J. 16: 740-749.
Süt Sanayisinde Biyofilm Oluşturan Mikroorganizmalar ve Biyofilm Oluşumunun Önlenmesi
Yıl 2011,
Cilt: 9 Sayı: 4, 12 - 16, 01.08.2011
Zübeyde Öner
Zeynep Ölmez
Öz
Bu çalışmada, süt sanayisinde biyofilm oluşumu üzerinde çalışılmıştır. Bu amaçla Burdur ve Isparta’da süt işletmelerinden biyofilm örnekleri toplanarak, mikroorganizma profili belirlenmiştir. İşletmeden alınan örneklerden izole edilen izolatlarla laboratuar koşullarında biyofilm oluşturulmuş ve oluşan biyofilm üzerine çeşitli ticari dezenfektanlar kullanılmıştır. Laboratuar koşullarında paslanmaz çelik yüzeyde oluşturulan biyofilmin temizlenmesi için uygun olan zaman ve konsantrasyonu tespit etmek için farklı konsantrasyonlarda ve farklı sürelerde işlem uygulanmıştır. Alınan örneklerden izole edilen mikroorganizma grupları şu şekilde bulunmuştur: Toplam mezofilik aerob bakteri grubu
Kaynakça
- [1] Mittelman, M.W., 1998. Structure and functional characteristics of bacterial biofilms in fluid processing operations. J.Dairy Sci. 81: 2760-2764.
- [2] Koutzayiotis, D., 1992. Bacterial biofilms in milk pipelines. S. Afr. J. Dairy Sci. 64: 19-22.
- [3] Flint, S.H., Bremer, P.J., Brooks, J.D., 1997. Biofilms in dairy manufacturing plant-description, current concerns and methods of control. Biofouling 11: 81-97.
- [4] Bremer, P., Suzanne Fillery, J., James McQuillan, A., 2006. Laboratory scale Clean-In-Place (CIP) studies on the effectiveness of different caustic and acid wash steps on the removal of dairy biofilms. Int. J. Food Microbiol. 106: 254-262.
- [5] Austin, J.W., Berferon, G., 1995. Development of bacterial biofilms in dairy processing lines. J.Dairy Res. 62: 509-519.
- [6] Amy, C., Wong, L., 1998. Biofilms in food processing environments. J. Dairy Sci. 81: 2765- 2770.
- [7] Halkman, A.K., 2005. Gıda Mikrobiyolojisi Uygulamaları. Başak Matbaacılık ve Tanıtım Hizmetleri Ltd. Şti. Ankara. 358p.
- [8] Sharma, M., Anand, S.K., 2002. Characterization of constitutive microflora of biofilms in dairy processing lines. Food Microbiol. 19: 627-636.
- [9] Bore, E., Langsrud, S., 2005. Characterization of microorganisms isolated from dairy industry after cleaning and fogging disinfection with alkyl amine and peracetic acid. J. of Appl. Microbiol. 98: 96-105.
- [10] Brown, M.R., Allison, D.G., Gilbert, P., 1988. Resistance of bacterial biofilms to antibiotics: a growth rate related effect. J. Antimicrob. Chemotherapy 22: 777-783.
- [11] Brading, M.G., Jass, J., Lappin-Scott, H., 2003. Dynamics of Bacterial Biofilm Formation. Microbial Biofilms. Edited by H.M.Lappin-Scott and J.W.Costerton Cambridge University pres.301p.
- [12] Frank, J.F., Koff, R.A., 1990. Surface adherent growth of Listeria monocytogenes is associated with increased resistance to surfactant sanitizers and heat. J. Food Protect. 53: 550-554.
- [13] Deibel, V., 2005. Biofilms. Internet Journal of Food Safety 1: 6-7.
- [14] Parkar, S.G., Flint, S.H., Brooks, J.D., 2003. Physiology of biofilms of thermophilic bacillipotential consequences for cleaning. J. Ind. Microbiol. Biotech. 30: 553-560.
- [15] Andersson, A., Rönner, U., Granum, P., 1995. What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens? Int. J. Food Microbiol. 28: 145-155.
- [16] Becker, H., Schaller, G., Wiese, W., Terplan, G., 1994. Bacillus cereus in infant foods and dried milk products. Int. J. Food Microbiol. 23: 1-15.
- [17] Shaheen, R., Svensson, B., Andersson, M.A., Christiansson, A., Salkinoja-Salonen, M., 2010. Persistence strategies of Bacillus cereus spores isolated from dairy silo tanks. Food Microbiol. 27: 347-355.
- [18] Wijnands, L.M., Dufrenne, J.B., Rombouts, F.M., Veld, P.H., Van Leusden, F.M., 2006. Prevalence of potentially pathogenic Bacillus cereus in food commodities in the Nether. J. Food Protec. 69: 2587-2594.
- [19] Banyko, J., Vyletelova, M., 2009. Determining the source of Bacillus cereus and Bacillus licheniformis isolated from raw milk, pasteurized milk and yoghurt. Letters in Appl. Microbiol. 48: 318-323.
- [20] Bartoszewicz, M., Hansen, B.M., Swiecicka, I., 2008. The members of the Bacillus cereus group are commonly present contaminants of fresh and heat-treated milk. Food Microbiol. 25: 588-596.
- [21] Svensson, B., Ekelund, K., Ogura, H., Christiansson, A., 2004. Characterization of Bacillus cereus isolated from milk silo tanks at eight different dairy plants. Int. Dairy J. 14: 17-27.
- [22] Svensson, B., Monthan, A., Shaheen, R., Andersson, A., Salkinoja-Salonen, M., Christiansson, A., 2006. Occurrence of emetic toxin producing Bacillus cereus in the dairy production chain. Int. Dairy J. 16: 740-749.